The PKH67 green fluorescent cell linker kit, horseradish peroxidase-conjugated anti-mouse immunoglobulin G, and horseradish peroxidase-conjugated anti-rabbit immunoglobulin G were obtained from Sigma-Aldrich (St. Louis, MO, USA). LPS (Escherichia coli 0111: B4) and fluorescein isothiocyanate dextran (FITC-dextran; 40,000 Da) were purchased from Sigma-Aldrich (St. Louis, MO, USA). IFN-γ was purchased from eBioscience (San Diego, CA). Recombinant Porcine IL-4 was obtained from Bio-Techne Co., Ltd. (Minneapolis, MN, USA). CD63, ALIX, and TSG101 were obtained from Abcam (Cambridge, UK). Annexin V-FITC/PI Apoptosis Detection Kit was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China).
Horseradish peroxidase conjugated anti mouse immunoglobulin g
Manufactured by Merck Group
Sourced in United States
Horseradish peroxidase-conjugated anti-mouse immunoglobulin G is a laboratory reagent that binds to mouse immunoglobulin G (IgG) antibodies. The horseradish peroxidase enzyme is conjugated to the anti-mouse IgG antibody, enabling the detection and visualization of mouse IgG in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Lps escherichia coli 0111 b4, by Merck Group (1 mentions)
Horseradish peroxidase conjugated anti rabbit immunoglobulin g, by Merck Group (1 mentions)
Pkh67 green fluorescent cell linker kit, by Merck Group (1 mentions)
Ifn γ, by Thermo Fisher Scientific (1 mentions)
Tsg101, by Abcam (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Vazyme (1 mentions)
Nitrocellulose membrane, by GE Healthcare (1 mentions)
Anti gfp clones 7 1 and 13, by Roche (1 mentions)
Anti pgk, by Thermo Fisher Scientific (1 mentions)
2 protocols using horseradish peroxidase conjugated anti mouse immunoglobulin g
1
Cellular Imaging and Vesicle Analysis Protocol
2
Immunoblotting Analysis of Yeast Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Yeast cells were grown as above-mentioned and crude protein extracts were prepared as previously described (50) . Nitrocellulose membranes (GE Healthcare Life Sciences) were probed with anti-GFP (clones 7.1 and 13.1, Roche) and anti-PGK (yeast 3-phosphoglycerate kinase, Invitrogen) antibodies, used at 1:3000 and 1:10000 dilutions, respectively. Primary antibodies were detected with horseradish peroxidase-conjugated anti-mouse immunoglobulin G (Sigma) by enhanced chemiluminescence.
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