Encapsulation was carried out using a B€ uchi mini B-290 spray dryer (B€ uchi Labortechnik AG, Switzerland) equipped with the standard 0.7 mm diameter nozzle. The inlet and outlet temperatures were 140 C and 70 ± 2 C, respectively. The air flow rate, rate of liquid feed, atomisation pressure were 600 L/h, 8 ml/min, and 0.55 bar, respectively.
Buchi mini b 290
Manufactured by Büchi
Sourced in Switzerland
The Buchi mini B-290 is a compact rotary evaporator designed for efficient solvent removal in laboratory settings. It features a digital display for temperature and rotation speed control, and is capable of operating at reduced pressure to facilitate the evaporation process. The Buchi mini B-290 is a versatile piece of lab equipment suitable for a range of applications requiring solvent removal.
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Lab products found in correlation
Multiskan go, by Thermo Fisher Scientific (2 mentions)
Ferric chloride, by Lach-Ner (1 mentions)
Sodium nitrite, by Lach-Ner (1 mentions)
Sil 20a autosampler, by Shimadzu (1 mentions)
Spd 20av uv vis detector, by Shimadzu (1 mentions)
Christ alpha 2 4 lsc, by Martin Christ (1 mentions)
Lc 20at binary pump, by Shimadzu (1 mentions)
Prominence chromatographic system, by Shimadzu (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Folin ciocalteau reagent, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
β carotene, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Maltodextrin, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
4 protocols using buchi mini b 290
1
Spray Drying Encapsulation Procedure
2
Spray Drying of Homogenized Mixture
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The homogenized mixture was spray dried using a laboratory scale spray dryer (Buchi mini B-290, Büchi Labortechnik, Switzerland) at an inlet temperature of 130 °C and an outlet temperature of 65 ± 2 °C. The spraying air flow rate and rate of liquid feed were 600 L/h and 8 mL/min, respectively.
3
Antioxidant Capacity Analysis of Whey Protein
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Folin-Ciocalteau reagent, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH●), β-carotene, pancreatin, pepsin, Trolox and trichloroacetic acid were purchased from Sigma Chemical Co. (St Louis, MO, USA), ferric chloride was obtained from J.T. Baker (Deventer, Holland), and sodium nitrite from LACH-NER (Brno, Czech Republic). Other chemicals and solvents were of the highest analytical grade. Whey protein isolate (WPI) was purchased from Olimp Laboratories (Debica, Poland). Distilled water was produced using water purification system DESA 0081 Water Still destilator (POBEL, Madrid, Spain). Absorbance in spectrophotometrical assays was measured on a Multiskan GO microplate reader (Thermo Fisher Scientific Inc., Waltham, MA, USA). For HPLC analysis a Shimadzu Prominence chromatographic system was used, which consisted of LC-20AT binary pump, CTO-20A thermostat and SIL-20A autosampler connected to the SPD-20AV UV/Vis detector (Shimadzu, Kyoto, Japan). Freeze-dryer, model Christ Alpha 2-4 LSC, was from Martin Christ (Osterode am Harz, Germany), and spray-dryer (Buchi mini B-290) from BüchiLabortechnik AG (Flawil, Switzerland).
4
Nanoencapsulation of Compounds via Spray Drying
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The nanoencapsulation was carried out according to the method of Hou et al. [34] using a solution of an emulsion of 1% EO with a quantity of 1% of Tween 80 (Sigma-Aldrich, Oakville, ON, Canada). Then, the mixtures were mixed with a high-speed homogenizer (Wilmington, DE, USA) at 10,000 rpm for 5 min. Then, the encapsulating agent (Maltodextrin, Sigma-Aldrich, Oakville, ON, Canada) was added at 5% (w/v) concentration. The mixture was introduced into the Buchi mini-B-290 spray dryer (Buchi, Flawil, Switzerland) to evaporate water. The inlet and outlet air temperatures were 120 and 52 • C, respectively. A feed flow of the solution to be dried at 2 mL/min, a spray air flow rate of 742 L/h and a pressure drop of 1.35 bar were used. The vacuum cleaner had a gas flow of 25 m 3 /h. To maintain the homogeneity of the solution, and the suspensions were gently shaken using magnetic stirring. After spray drying, the powder was collected, weighed and stored in a closed metal bag under a vacuum [35].
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