Blood samples were collected from all participants in tubes without anticoagulants on the same day as the ultrasound examination during the early follicular phase. Serum FSH levels were measured using an Elecsys FSH assay (Roche Diagnostics Corp., Indianapolis, IN, USA) and serum LH levels were measured using an Elecsys LH assay (Roche Diagnostics Corp.). Insulin levels were measured using an Elecsys Insulin assay (Roche Diagnostics Corp.), and fasting glucose levels and levels during a standard 2-hour 75-gram oral glucose tolerance test were measured using L-Type GluI (Wako Pure Chemical Industries, Osaka, Japan). The total cholesterol and triglyceride levels were measured using a Pureauto S (Sekisui Medical, Tokyo, Japan), and high density lipoprotein and low density lipoprotein were measured using a Cholestest (Sekisui Medical, Tokyo, Japan). Both the intraassay and interassay coefficients of variation for all measurements were less than 5%.
Cholestest
Manufactured by Sekisui
Sourced in Japan
Cholestest is a laboratory equipment designed for the measurement of cholesterol levels. It provides accurate and reliable results for clinical analysis.
Automatically generated - may contain errors
4 protocols using cholestest
1
Biochemical Profiling of Participants
2
Lipid Profiling in Mouse Liver
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Lipids were extracted from liver tissue (approximately 100 mg per mouse) according to Folch's method with chloroform/methanol 25 (link). Triglyceride and total cholesterol were quantified using Cholestest® (Sekisui Medical Corp. Tokyo, Japan). Total fatty acid content (free and esterified, μg/g) in the liver tissue was measured by gas chromatography (GC profiles) with the samples prepared by chloroform and methanol using GC-2010 Plus (Shimadzu, Kyoto, Japan).
3
Retinal Lipid Extraction and Quantification
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After pressure loading, the retina was detached from sclera. Total lipids were extracted from the retina according to Folch’s method with chloroform/methanol54 (link), and quantified using Cholestest® (Sekisui Medical Corp. Tokyo, Japan).
4
Detailed Lipid Biomarker Measurement Protocol
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A central committee, composed of the chief medical officers of all 13 participating districts, developed a detailed manual for data collection. Body weight was recorded with the subjects clothed. Height was measured with stockinged feet. Body mass index (BMI) was calculated as weight (kg)/height (m2). Blood samples were taken after overnight fasting. TC was measured via a cholesterol dehydrogenase-ultraviolet method. Triglycerides (TG) was measured using an enzymatic method. LDL-C and high-density lipoprotein cholesterol (HDL-C) were measured by direct methods using a commercial kit (Cholestest from Sekisui Medical, Tokyo, Japan). SdLDL-C level was directly and selectively measured using a commercial kit (sdLDL-EX from Denka Seiken, Tokyo, Japan). An external laboratory (SRL, Tokyo, Japan) measured the serum lipid markers. The markers were measured by the standardised programme proposed by the Clinical and Laboratory Standards Institute. The nonHDL-C was calculated by subtracting HDL-C from TC. Information about medical history, lifestyle and menopausal status were obtained with a self-reported questionnaire. Smoking status was classified as smoking, former smoking, or never smoking.
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