Poch 100iv diff

Blood samples (5 ml) were collected from the cephalic or jugular vein and were stored in EDTA anticoagulant tubes. Absolute red blood cell, leukocyte, and platelet counts were determined using a Poch-100Iv Diff (Sysmex, São Paulo, Brazil) veterinary automatic cell counter, whereas differential leukocyte counts and the morphological analysis of cells were obtained and performed, respectively, by two different and experienced pathologist veterinarians, under a microscope, using a freshly stained Rosenfeld slide.
An average of blood count data was used to calculate the inflammatory indices: NRL (n° neutrophils/n° lymphocytes), PLR (n° platelets/n° lymphocytes), MLR (n° monocytes/n° lymphocytes), and SII (n° platelets × NRL).
Blood was stored in a tube (with or without clot activator), and serum was separated up to 24 h after collection and stored at −80°C until use. LDH (kinetic determination) and albumin (bromocresol green) were measured using an automated device (Chem Well T; Labtest Interteck, Minas Gerais, Brazil) with reagents supplied by the manufacturer. CRP levels were determined using a chemistry immunoassay technique (Catalyst One, Idexx, São Paulo, Brazil). The CRP/ALB ratio was calculated by dividing the CRP by the albumin value.

Eight healthy beagles owned by our laboratory (male/female: 4/4; age [mean ± standard deviation]: 1.4 ± 0.1 years; body weight [mean ± standard deviation]: 9.9 ± 1.0 kg) were used in this study. Each dog was determined to be healthy based on the findings of a complete physical examination, complete blood cell count (poch-100iV Diff, Sysmex Corporation, Hyogo, Japan), serum blood chemistry profile (FDC7000V, FUJIFILM Corporation, Kanagawa, Japan), electrocardiography (D320, FUKUDA M-E KOGYO Co, LTD, Tokyo, Japan), thoracic and abdominal radiography (FCR PRIMA V and FD0078-V Station T, FUJIFILM Corporation, Kanagawa, Japan), transthoracic and abdominal ultrasonography (Vivid iq, GE Healthcare, Tokyo, Japan), and oscillometric blood pressure measurement (BP100DII, FUKUDA M-E KOGYO Co, LTD, Tokyo, Japan).

To monitor the health status of piglets, body temperatures and clinical scores were systematically recorded 3 times a day, starting at 3 days before inoculation to record baseline levels. Swelling of joints, particularly carpal, tarsal and knee joints and occurring lameness were assessed and interpreted as arthritis. Pigs were restraint and blood was collected from the jugular vein on a daily basis starting 3 days before inoculation to monitor white blood cell counts (WBC), bacteraemia and to collect plasma. WBCs were counted using an automated cell counter (Sysmex, pocH-100iV-diff), including differentiation of blood cells.