The human neuroblastoma cell line, IMR-32, was obtained from the A.T.C.C. (LGC Standards Sp. z.o.o., Poland). The cells were maintained in a humidified atmosphere containing 5% CO2 and 95% humidified air at 37°C in Dulbecco's modified Eagle's medium (DMEM) with L-glutamine (PanEco, Russia) supplemented with 10% fetal bovine serum (FBS) (PanEco), and gentamicin (50 mkg/ml) (Veropharm, Russia).
Imr 32
Manufactured by LGC
Sourced in Brazil
The IMR-32 is a compact and versatile laboratory instrument designed for performing infrared spectroscopy analyses. It features a sensitive infrared detector and advanced optics to capture detailed spectral data from a range of sample types. The core function of the IMR-32 is to accurately measure and analyze the absorption and reflectance characteristics of materials in the infrared region of the electromagnetic spectrum.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
L glutamine, by PanEco (1 mentions)
Sh sy5y, by American Type Culture Collection (1 mentions)
Sk n sh, by American Type Culture Collection (1 mentions)
Be 2 m17, by American Type Culture Collection (1 mentions)
Hff 1, by LGC (1 mentions)
2 protocols using imr 32
1
Culturing Human Neuroblastoma IMR-32 Cells
2
Cultivation of Human NB Cell Lines
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Human NB cells (Be(2)-M17, CHLA-20, IMR-32, SK-N-SH, and SH-SY5Y) and human foreskin fibroblasts (HFF-1) were cultivated in Dulbecco's Modified Eagle Medium (DMEM)/Ham's F-12 medium (LGC Biotechnology, São Paulo, Brazil) supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 IU/mL penicillin, and 0.1 mg/mL streptomycin (all from Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Cells were maintained at 37 °C in 5% CO 2 . CHLA-20 and IMR-32 NB cells were kindly gifted by St. Jude Children's Hospital (Memphis, TN, USA), SK-N-SH, Be(2)-M17, and SH-SY5Y were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). HFF-1 was obtained from the Rio de Janeiro Cell Bank, RJ, Brazil.
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