Poly-D-lysine hydrobromide, ferulic acid (99%), vanillin (99%), vanillic acid (97%), 3-(4,5-dimethylthialzal-z-yl)-2,5-diphenylterazolium (MTT), DCFH-DA probes, and Nutrient Mixture F-12 Ham were purchased from the Sigma-Aldrich Shanghai Trading Co. (Shanghai, China). Curcumin (98%) was obtained from the Aladdin Industrial Corporation (Shanghai, China). Fetal bovine serum (FBS) was bought from (Gibco BRL, Grand Island, NY, USA). Horse serum was bought from Solarbio Biological Technology Co., Ltd. (Beijing, China). Hydrogen peroxide (H2O2) was obtained from Shuangshuang Chemical Co., Ltd. (Yantai, China). An annexin V-FITC/PI apoptosis detection kit was obtained from Bibo Biological Technology Co., Ltd. (Nanjing, China). A Bradford protein assay kit, caspase-3 activity assay kit, caspase-9 activity assay kit, and cell MDA assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other reagents used were of analytical grade.
Caspase 9 activity assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Caspase-9 activity assay kit is a laboratory tool used to measure the activity of the caspase-9 enzyme. Caspase-9 is a key regulator of apoptosis, a process of programmed cell death. The kit provides the necessary reagents and protocols to quantify the enzymatic activity of caspase-9 in cell or tissue samples.
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Lab products found in correlation
Caspase 3 activity assay kit, by Nanjing Jiancheng (2 mentions)
Cell mda assay kit, by Nanjing Jiancheng (1 mentions)
Bradford protein assay kit, by Nanjing Jiancheng (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Horse serum, by Solarbio (1 mentions)
Glutathione reductase assay kit, by Nanjing Jiancheng (1 mentions)
Ldh cytotoxicity kit, by Nanjing Jiancheng (1 mentions)
2 protocols using caspase 9 activity assay kit
1
Antioxidant and Apoptotic Effects of Natural Compounds
2
Assaying Cellular Cytotoxicity and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The LDH release, GSH content, caspase-3 activity and caspase-9 activity were determined using the LDH cytotoxicity kit, glutathione reductase assay kit, caspase-3 activity assay kit and caspase-9 activity assay kit (Nanjing JianCheng Bio Institute, Nanjing, China), respectively, according to the manufacturer protocol [18 (link),27 (link),28 (link)].
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