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Aflatoxin g1

Manufactured by Pribolab
Sourced in China, Singapore

Aflatoxin G1 is a mycotoxin produced by certain Aspergillus fungi. It is a chemical compound that can be detected and quantified using analytical laboratory equipment.

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2 protocols using aflatoxin g1

1

Quantitative Analysis of Aflatoxins

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Five ml of a certified mix of four standard aflatoxin solutions containing an equal ratio of the four aflatoxins [Aflatoxin B1 (AFB1), Aflatoxin B2 (AFB2), Aflatoxin G1 (AFG1), and Aflatoxin G2 (AFG2)] was supplied by Pribolab, China (STD#1089). Two sets of dilutions of the mixed aflatoxin standard solution were prepared in (75% MeOH) (first set) and 100% HPLC-grade acetonitrile (ACN) (A998-4, Fisher Scientific Malaysia) solvent (second set). Each set contains 16 concentration levels (0, 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, and 90 ng/ml) of the mixed aflatoxin standard solution. Each concentration was prepared in 7 different vials, making a total of 112 vials for each set (first set and second set).
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2

Electrochemical Detection of Aflatoxins

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Potassium ferricyanide and potassium ferrocyanide were obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). We obtained rGO, chloroauric acid, triclofos sodium, and 6-mercaptoethanol (MCH) from Shanghai Aladdin Bio-Chem Technology Co., Ltd (Shanghai, China). The sequences (Table 1) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). We obtained AFM1, aflatoxin M2, aflatoxin B1, aflatoxin G1 and aflatoxin G2 (>99%) from Pribolab Pte. Ltd. (Singapore). The sample of pure milk was purchased from Yonghui Supermarket (Chongqing, China).
All electrochemical measurements were conducted on the CHI600E workstation (Shanghai CH Instruments, China). The 3-electrode cell was used, where the GCE was chosen as the working electrode, the saturated calomel electrode as the reference electrode, and the platinum wire as the counter-electrode. Scanning electron microscopy was conducted using a Gemini SEM 300 scanning electron microscope (Carl Zeiss AG, Germany). Deionized water was purified using a Millipore (Bedford, MA) Milli-Q Ultrapure Water System and used for all experiments.
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