HeLa, MCF7, HEK-293T, MCF10A, and BT-549 cells were purchased from ATCC. HeLa, MCF7, and HEK-293T cells were maintained in DMEM (Nissui Pharmaceutical) supplemented with 8% FBS (Biowest) in an atmosphere with 5% CO2. MCF10A cells were maintained in DMEM/F-12 (Sigma-Aldrich) supplemented with 5% FBS, 20 ng/mL human EGF (PeproTech), 10 μg/mL human insulin, and 0.5 μg/mL hydrocortisone (Tokyo Chemical Industry) in an atmosphere with 5% CO2. BT-549 cells were maintained in RPMI1640 (Nissui pharmaceutical) supplemented with 8% FBS and 10 μg/mL human insulin (Sigma-Aldrich) in an atmosphere with 5% CO2. Cell line identities were verified using the GenomeLab Human STR Primer set (Beckman Coulter). Cells were routinely stained by Hoechst 33342 and no mycoplasma contamination was suspected. Cells were used for experiments within 20 passages from obtaining.
Hek 293t
Manufactured by Nissui Pharmaceutical
Sourced in Japan
HEK-293T is a cell line derived from human embryonic kidney cells. It is a widely used laboratory tool for various applications, including the production and study of recombinant proteins, virus research, and gene expression analysis.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Nissui Pharmaceutical (2 mentions)
Hydrocortisone, by Tokyo Chemical Industry (1 mentions)
Human insulin, by Merck Group (1 mentions)
Mcf 10a, by Merck Group (1 mentions)
Genomelab human str primer set, by Beckman Coulter (1 mentions)
Mcf 7, by Nissui Pharmaceutical (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Human egf, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Bt549, by American Type Culture Collection (1 mentions)
Dmem f12, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Rpmi 1640, by Nissui Pharmaceutical (1 mentions)
Hek293t, by RIKEN BioResource Center (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Ht 1080, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Nahco3, by Fujifilm (1 mentions)
Penicillin g, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
2 protocols using hek 293t
1
Maintenance of Human Cell Lines
2
Culture and Maintenance of HT1080 and HEK293T Cell Lines
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The human fibrosarcoma cell line HT1080 was obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) and the human embryonic kidney (HEK) cell line HEK293T was obtained from RIKEN BioResource Center (Tsukuba, Japan). HT1080 and HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM; catalogue no. 05919; Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) supplemented with 5% (v/v) fetal bovine serum (Bovogen Biologicals Pty Ltd., Melbourne, Australia), 100 mg/l kanamycin (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin G (Sigma-Aldrich), 600 mg/l L-glutamine (Sigma-Aldrich) and 2.25 g/l NaHCO3 (Wako Pure Chemical Industries, Ltd., Osaka, Japan). Cells were incubated at 37°C in a humidified incubator with 5% CO2.
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