Chromobacterium violaceum (ATCC 31532), Pseudomonas aeruginosa ATCC 700829, ATCC 9027, ATCC 15442, E. coli strains ATCC 10536 and ATCC 700928 were all purchased from American type Culture Collection (ATCC; Wesel, Germany) and C. violaceum CV026 (NCTC 13278) from Public Health England′s National Culture of Type Collection (NCTC; Salisbury, UK). Pseudomonas aeruginosa PAO1 was obtained from University of Helsinki, HAMBI collection (http://www.helsinki.fi/hambi/ ).
Atcc 15442
Manufactured by American Type Culture Collection
Sourced in Germany, France
ATCC 15442 is a lyophilized culture of Pseudomonas aeruginosa. It is a Gram-negative, aerobic bacterium commonly used as a test organism in the evaluation of antimicrobial agents and disinfectants.
Automatically generated - may contain errors
3 protocols using atcc 15442
1
Bacterial Strain Acquisition from ATCC and NCTC
2
Well-Characterized Pseudomonas Strains: A Cornerstone for Research
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Four well-described and genome-available reference strains were used in the present study, ATCC27853 and ATCC15442, obtained from the American Type Culture Collection (ATCC), and PAO1 and PA14, from the collection of Institut Pasteur (Paris, France). Strain ATCC15442 is recommended for disinfectant susceptibility testing44 , strain ATCC27853 is the Pseudomonas spp. reference for antibiotic susceptibility testing45 , PAO1 is the reference genome for the P. aeruginosa species46 (link) and strain PA14 is a highly virulent isolate representing the most P. aeruginosa common clonal group worldwide contrary to PAO147 (link).
3
Cultivation of Pseudomonas aeruginosa Strains
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The study utilized five strains of Pseudomonas aeruginosa: PAO1, ATCC 10145, ATCC 15442 from the American Type Culture Collection and DBM 3081, and DBM 3777 which were obtained from the Collection of Microorganisms of the Department of Biochemistry and Microbiology, UCT Prague. Glycerol cryopreserves of these strains were stored at −70 °C. These strains represent a wide range of phenotypes from a soil isolate to the most researched clinical isolate, PAO1.
Prior to each experiment, all P. aeruginosa strains were precultivated in a Luria–Bertani (LB) liquid medium at 37 °C for 24 hours to achieve the exponential growth phase. The cultivation was carried out in Erlenmeyer flasks with a volume of 100 mL and agitation at 150 rpm.
Prior to each experiment, all P. aeruginosa strains were precultivated in a Luria–Bertani (LB) liquid medium at 37 °C for 24 hours to achieve the exponential growth phase. The cultivation was carried out in Erlenmeyer flasks with a volume of 100 mL and agitation at 150 rpm.
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