Anti-IL-1β, Anti-Caspase1, TNF-α were purchased from R&D Systems (Minneapolis, MN, USA); Anti-cleaved caspase1, Anti-NLRP3, Anti-IκBα, Anti-phospho-P38, Anti-phospho-ERK, Anti-phospho-JNK, Anti-phospho-IKKα/β, and Anti-Tublin were purchased from Cell Signaling Technology (Boston, MA, USA); Anti-TAK1, Anti-phospho-TAK1, Anti-ASC and recombinant active caspase1 protein were purchased from Abcam (Shanghai, China). The ultrapure LPS and Nigericin were obtained from Sigma-Aldrich (St. Louis, MO, USA). The caspase1 activity assay kit was purchased from Beyotime Biotechnology (Shanghai, China). The Human IL-1β ELISA Kit was purchased from Boster Biological Technology (Wuhan, China).
Human il 1β elisa kit
Manufactured by Boster Bio
Sourced in China
The Human IL-1β ELISA Kit is a laboratory tool used to detect and quantify the levels of interleukin-1 beta (IL-1β), a pro-inflammatory cytokine, in human samples. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of IL-1β in the tested sample.
Automatically generated - may contain errors
Lab products found in correlation
Ultrapure lps, by Merck Group (1 mentions)
Anti asc, by Abcam (1 mentions)
Anti tublin, by Cell Signaling Technology (1 mentions)
Anti caspase1, by R&D Systems (1 mentions)
Caspase 1 activity assay kit, by Beyotime (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
Nigericin, by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Anti phospho ikkα β, by Cell Signaling Technology (1 mentions)
Anti tak1, by Abcam (1 mentions)
Anti nlrp3, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 1, by Cell Signaling Technology (1 mentions)
Anti il 1β, by R&D Systems (1 mentions)
Anti phospho jnk, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti iκbα, by Cell Signaling Technology (1 mentions)
2 protocols using human il 1β elisa kit
1
Inflammasome Activation Pathway Assay
2
Measuring IL-1β in LPS-treated Cells
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The levels of IL-1β were detected by the human IL-1β ELISA kit (Boster, Shanghai, China) in AC16 cells. The cells were transfected with miRNA mimics for 12 h followed by LPS treatment for another 12 h. The levels of IL-1β were determined by specific enzyme immunoassay kits in accordance with the manufacturer’s instructions.
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