Slides were fixed in 4% paraformaldehyde in 13 PBS, blocked in Superblock (Thermo Scientific), and then incubated overnight at 4°C in 1:200 monoclonal mouse immunoglobulin G (IgG) 2b anti-human-HuC/D (Invitrogen) or 1:500 polyclonal rabbit IgG anti-GFP (Molecular Probes) in PBS (5% horse serum, 0.16% Triton X-100 in 1X PBS). Slides were then incubated in 1:500 goat anti-mouse Alexa 568 IgG (Molecular Probes) or 1:500 donkey anti-rabbit Alexa 488 IgG (Molecular Probes), DAPI stained, and coverslipped with Gelvatol.
Polyclonal rabbit igg anti gfp
Manufactured by Thermo Fisher Scientific
The Polyclonal rabbit IgG anti-GFP is a laboratory reagent used to detect the presence of green fluorescent protein (GFP) in samples. It is an antibody produced in rabbits that specifically binds to GFP, allowing for the identification and localization of GFP-tagged proteins in various experimental settings.
Automatically generated - may contain errors
2 protocols using polyclonal rabbit igg anti gfp
1
Immunofluorescent Labeling of Cultured Cells
2
Immunofluorescent Labeling of Cultured Cells
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