BLI was performed once a week until the end of the study (Day 28) using an IVIS-Lumina II (Perkin Elmer, France) generating a pseudo-colored image representing light intensity and superimposed over a greyscale reference image. Each mouse was IP injected with 100mg/kg luciferin potassium salt (Promega, France). Mice anesthetized by 1.5% isoflurane were placed on a thermostatically controlled heating pad (37°C) during imaging. Acquisition binning and duration were set depending on tumor activity. Signal intensity was quantified as the total flux (photons/seconds) within ROIs drawn manually around the tumor area using Living Image 4.0 software (Perkin Elmer, France). The sum of signals from the prone and supine positions was considered for each mouse.
Luciferin potassium salt
Manufactured by Promega
Sourced in France, United States
Luciferin potassium salt is a chemical compound used in bioluminescence assays. It serves as a substrate for firefly luciferase enzyme, which catalyzes a light-emitting reaction. This salt is commonly used in research applications involving ATP detection and quantification.
Automatically generated - may contain errors
Lab products found in correlation
Living image 4, by PerkinElmer (1 mentions)
Ivis lumina 2, by PerkinElmer (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Genview (1 mentions)
Egfp mrna, by TriLink (1 mentions)
Cleancap firefly luciferase mrna, by TriLink (1 mentions)
Tae buffer, by Sangon (1 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Hepg2 cells, by National Collection of Authenticated Cell Cultures (1 mentions)
2 protocols using luciferin potassium salt
1
In Vivo Bioluminescence Imaging Protocol
2
Formulation and Characterization of mRNA Lipoplexes
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All peptides in this study (Table 1 ) were purchased from ChinaPeptides (Shanghai, China). CleanCap® firefly luciferase mRNA, EGFP mRNA, and cyanine-5 EGFP mRNA were purchased from TriLink Bio Technologies (San Diego, CA, USA). LipofectamineTM 2000 transfection reagent was purchased from Invitrogen (Carlsbad, USA). Luciferin potassium salt was purchased from Promega (Madison, WI, USA). Dulbecco’s modified Eagle’s medium (DMEM), 0.25% Trypsin-EDTA, penicillin/streptomycin antibiotics, and PBS (pH 7.2–7.4) were purchased from Genview (Florida, USA). Fetal Bovine Serum (FBS, South America origin) and Opti-MEM I reduced serum medium were purchased from Gibco (New York, USA). DNA Gel Loading Dye (6 ×) was purchased from Biosharp (Anhui, China). GelRed nucleic acid stain was purchased from US Everbright (California, USA). 1 × Tris-acetate EDTA (TAE) buffer was diluted from 50 × TAE buffer (Sangon Biotech, Shanghai, China) using distilled water. A549 and HepG2 cells were purchased from China Center for Type Culture Collection (CCTCC, Shanghai, China). Other reagents were obtained from Sigma-Aldrich (Saint Louis, MO, USA) of analytical grade or better grade.
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