Fluticasone

Manufactured by Merck Group

Sourced in Germany, United States

Fluticasone is a synthetic glucocorticoid compound used in the manufacture of various pharmaceutical products. It is a white to off-white crystalline powder with a molecular formula of C22H28F2O5 and a molecular weight of 418.47 g/mol. Fluticasone is commonly used as an active ingredient in the development of inhaled and topical corticosteroid medications.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using fluticasone

Inhibiting Mucin Production in Virus-Induced Airway Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

In experiments evaluating the effect of tiotropium (a gift from Boehringer Ingelheim, Ingelheim Germany) or fluticasone (Sigma-Aldrich, Zwijndrecht, The Netherlands) on HRV-induced mucin production, cells were pre-treated by incubation in HC-free complete medium containing tiotropium (10–1,000 nM) or fluticasone (10–1,000 nM) prior to infection with HRV. After infection, the basal medium was replaced by fresh free-HC medium containing tiotropium or fluticasone.
In experiments aimed to investigate the role of extracellular ATP release in virus-induced mucin production, suramin (a non-selective P2R antagonist, TOCRIS) was added to basal media at 10 μM for 30 min prior to HRV treatment. To investigate the ability of tiotropium and fluticasone to inhibit ATP- induced mucin expression, cells pre-treated with tiotropium/fluticasone were stimulated with 100 μM ATP at the basal side for 24 h.
In experiments with DAPT (γ-secretase inhibitor, TOCRIS), ALI-PBEC were incubated in complete medium supplemented with either 5 μM DAPT or solvent control (0.1% DMSO) during differentiation for 14 days. On day 14, cells were infected with HRV-A16 at MOI 1, and following infection the basal medium was replaced by fresh HC-free medium containing 5 μM DAPT or solvent control and cells were incubated for another 24 h.

Wang Y., Ninaber D.K., van Schadewijk A, & Hiemstra P.S. (2020). Tiotropium and Fluticasone Inhibit Rhinovirus-Induced Mucin Production via Multiple Mechanisms in Differentiated Airway Epithelial Cells. Frontiers in Cellular and Infection Microbiology, 10, 278.

+ Open protocol

+ Expand

Screening Diverse Compounds for Antiviral Efficacy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Selection of compounds for testing was based on side effect profiles and compound availability. Bacampicillin (B0070000), ciclopirox (SML2011–50MG), ciclosporin (C2163000), clofazimine (1138904–200MG), dicycloverine (D1060000), fludrocortisone (1273003–200MG), fluticasone (1285873–100MG), haloperidol (H1512–5G), isoxicam (I1762–1G), lansoprazole (1356916–150MG), metixene (M1808000), myricetin (M6760–10MG), pentoxifylline (1508901–200MG), sirolimus (S-015–1ML), tretinoin (1674004–5X30MG), and valproic acid (1708707–500MG) were purchased from Sigma-Aldrich. Remdesivir (GS-5734) was purchased from Selleckchem. Compounds were resuspended in DMSO according to manufacturer’s instructions and serially diluted to the relevant concentrations for treatment of infected cells.

Le B.L., Andreoletti G., Oskotsky T., Vallejo-Gracia A., Rosales R., Yu K., Kosti I., Leon K.E., Bunis D.G., Li C., Kumar G.R., White K.M., García-Sastre A., Ott M, & Sirota M. (2021). Transcriptomics-based drug repositioning pipeline identifies therapeutic candidates for COVID-19. Research Square.

+ Open protocol

+ Expand

Compound Selection and Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Selection of compounds for testing was based on side effect profiles and compound availability. Bacampicillin (B0070000), ciclopirox (SML2011-50MG), ciclosporin (C2163000), clofazimine (1138904-200MG), dicycloverine (D1060000), fludrocortisone (1273003-200MG), fluticasone (1285873-100MG), haloperidol (H1512-5G), isoxicam (I1762-1G), lansoprazole (1356916-150MG), metixene (M1808000), myricetin (M6760-10MG), pentoxifylline (1508901-200MG), sirolimus (S-015-1ML), tretinoin (1674004-5X30MG), and valproic acid (1708707-500MG) were purchased from Sigma-Aldrich. Remdesivir (GS-5734) was purchased from Selleckchem.
Compounds were resuspended in DMSO according to manufacturer’s instructions and serially diluted to the relevant concentrations for treatment of infected cells.

+ Open protocol

+ Expand

Myogenic Culture and Transplantation of MuSCs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following FACs isolation, we resuspended MuSCs in myogenic cell culture medium containing DMEM/F10 (50:50), 15% FBS, 2.5 ng ml⁻¹ fibroblast growth factor-2 and 1% penicillin-streptomycin. We added the indicated doses (see respective Figure legends) 50 nM of SAG1.3 (Cayman Chemical Company, catalog # 11914), 1 µM of GSA-10 (Sigma-Aldrich, catalog # SML1171), 25 nM of Fluticasone (Sigma-Aldrich, catalog # F9428), 5 µM of Cyclopamine (STEMCELL Technologies, catalog #72072), 1 µM Purmorphamine (Selleck Chemical, catalog # S3042), 100 nM of Vismodegib (Selleck Chemicals, catalog # S1082) or 0.1 µg/ml SHH (R&D Systems, catalog # 461-SH-025) to MuSCs cultured on collagen coated dishes for the first 24 h. The cells were then trypsinized and cells reseeded onto hydrogels for an additional 6 days of culture. All treatments were compared to their solvent (DMSO) vehicle control.
For IFT88^−/− MuSCs transplant studies, we infected MuSCs with lentivirus encoding elongation factor-1α promoter-driven luc-IRES-GFP (GFP/luc virus) for 24 h in culture as described previously^{6 (link)}. Cells were assayed for GFP 48 h post-infection using an inverted fluorescence microscope (Carl Zeiss Microimaging).

Palla A.R., Hilgendorf K.I., Yang A.V., Kerr J.P., Hinken A.C., Demeter J., Kraft P., Mooney N.A., Yucel N., Burns D.M., Wang Y.X., Jackson P.K, & Blau H.M. (2022). Primary cilia on muscle stem cells are critical to maintain regenerative capacity and are lost during aging. Nature Communications, 13, 1439.

+ Open protocol

+ Expand

Bronchial Epithelial Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Normal human bronchial epithelial cells (NHBE, Lonza) were maintained in Keratinocyte SFM basal medium supplemented with 5 μg/L human recombinant epithelial growth factor (EGF), 50 mg/L bovine pituitary extract (BPE), 5 mg/L insulin, and 25 nM hydrocortisone at 37°C in a 5% CO₂ atmosphere. Cells were passaged every 3 days and plated for experimental treatment within 6 passages.
NaAsO₂, montelukast, fluticasone, N-acetylcysteine, and BAY117082 were purchased from Sigma–Aldrich (United States). Antibodies against fibronectin (Sigma, F3648), MMP-2 (GeneTex, GTX 104577), GAPDH (GeneTex, GTX100118), SMAD2/3 (GeneTex, GTX111123), β-catenin (R&D, AF1329), N-Cadherin (GeneTex, GTX127345), and E-Cadherin (GeneTex, GTX100443) were used.

Chen H.C., Chiou H.Y., Tsai M.L., Chen S.C., Lin M.H., Chuang T.C., Hung C.H, & Kuo C.H. (2022). Effects of Montelukast on Arsenic-Induced Epithelial-Mesenchymal Transition and the Role of Reactive Oxygen Species Production in Human Bronchial Epithelial Cells. Frontiers in Pharmacology, 13, 877125.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!