Glycine, and hydrochloric acid (HCl) were purchased from Merck (Germany) and β-lactoglobulin (BLG) with 90 % purity was purchased from Sigma-Aldrich (USA). All the solutions were prepared with Milli-Q water and the pH was determined using an Iskra pH meter (model MA 5740) and a combined glass microelectrode InLab 423 (Mettler Toledo). The 20 mM Glycine buffer at pH 2 was prepared by dissolving an appropriate amount of Glycine in water filled glass beaker and then the pH was adjusted by adding a few drops of 1 M HCl. The buffer was transferred to a volumetric flask and diluted to the final concentration. The buffer solutions were filtered through 0.45 μm filter (Sartorious) before use. The aqueous solutions at pH 2 were prepared in the same way and the pH was readjusted after the BLG was dissolved if necessary. Solutions were continuously stirred during all steps of sample preparation.
Inlab 423
Manufactured by Mettler Toledo
Sourced in Switzerland
The InLab 423 is a pH electrode designed for laboratory use. It features a glass body and a 3-in-1 electrode with a pH sensor, reference electrode, and temperature sensor. The electrode is suitable for use in aqueous solutions.
Automatically generated - may contain errors
4 protocols using inlab 423
1
Glycine-HCl Buffer for BLG Studies
2
HeLa Cell Metabolism Monitoring Using MOCSERs
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Sixteen channels per device
were measured simultaneously using a Keithley 2636A source measure
unit and a home-built switch control box, which was controlled and
monitored by a LabVIEW program. The HeLa cellular metabolic activity
was measured over 6 h using pulsed cycles at a constant source–drain
voltage of 0.6 V. Within the pulses, the “on” state
was set to 3 min (the time needed for the signal to stabilize), whereas
the “off” state was set to 20 min to reduce the electrical
load on the cells, yet enough to maintain measurable electrical currents
and responses.
To test the device’s sensitivity in the
CO2-independent medium solution, calibration plots were
measured for various pH solutions. All pH measurements throughout
the experiments were performed using a commercial pH meter (portable
pH-meter 1120 with a 3 mm InLab 423 combination pH micro electrode,
Mettler Toledo International, Inc.). Moreover, source–drain
current (Isd) versus voltage (Vsd) characteristics at various gate voltages
(Vg) were applied using an external gate.
Here, to apply gate voltages without currents, a Pt wire electrode
was coated with a SixNy dielectric layer of 300 nm, using a plasma-enhanced chemical
vapor deposition tool. These measurements were conducted both on bare
MOCSERs and on MOCSERs coated with a monolayer of HeLa cells.
were measured simultaneously using a Keithley 2636A source measure
unit and a home-built switch control box, which was controlled and
monitored by a LabVIEW program. The HeLa cellular metabolic activity
was measured over 6 h using pulsed cycles at a constant source–drain
voltage of 0.6 V. Within the pulses, the “on” state
was set to 3 min (the time needed for the signal to stabilize), whereas
the “off” state was set to 20 min to reduce the electrical
load on the cells, yet enough to maintain measurable electrical currents
and responses.
To test the device’s sensitivity in the
CO2-independent medium solution, calibration plots were
measured for various pH solutions. All pH measurements throughout
the experiments were performed using a commercial pH meter (portable
pH-meter 1120 with a 3 mm InLab 423 combination pH micro electrode,
Mettler Toledo International, Inc.). Moreover, source–drain
current (Isd) versus voltage (Vsd) characteristics at various gate voltages
(Vg) were applied using an external gate.
Here, to apply gate voltages without currents, a Pt wire electrode
was coated with a SixNy dielectric layer of 300 nm, using a plasma-enhanced chemical
vapor deposition tool. These measurements were conducted both on bare
MOCSERs and on MOCSERs coated with a monolayer of HeLa cells.
3
pH Measurement with Glass Microelectrode
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Values of media pH were determined using a glass pH microelectrode (InLab 423, Mettler Toledo, Switzerland).
4
Glycine-HCl Buffer for BLG Studies
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