Anti pd l2

Manufactured by BioLegend

Anti–PD-L2 is a lab equipment product from BioLegend. It is a monoclonal antibody that binds to the PD-L2 (Programmed Death-Ligand 2) protein.

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Lab products found in correlation

Anti f4 80, by BioLegend (3 mentions) Anti pd l1, by BioLegend (3 mentions) Anti hla abc apc, by Thermo Fisher Scientific (1 mentions) Anti cd54 pe, by Beckman Coulter (1 mentions) Anti pd l1 pe, by BioLegend (1 mentions) Anti mitf, by Santa Cruz Biotechnology (1 mentions) Anti hmb 45, by Agilent Technologies (1 mentions) Anti hla dr pecy7, by Beckman Coulter (1 mentions) Mouse anti tyrosinase, by Santa Cruz Biotechnology (1 mentions) Anti cd3, by BD (1 mentions) Cellquest software, by BD (1 mentions) Facscalibur system, by BD (1 mentions) Rpe conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Pe conjugated anti pd l1, by BioLegend (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Normal mouse serum, by Thermo Fisher Scientific (1 mentions) Apc annexin 5 apoptosis detection kit with pi, by BioLegend (1 mentions) Facs lsr fortessa flow cytometer, by BD (1 mentions) Anti mouse cd16 32, by BioLegend (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

PD-L2 (6 citations) Anti-PD-L2 PE (3 citations) Anti-PD-L2-APC (3 citations) Anti-PD-L2: 24F.10C12 (2 citations)

6 protocols using anti pd l2

Murine Monoclonal Antibodies for Immunodetection

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The following murine mAbs were used for IHC: W6/32 to detect HLA class I antigens (Dianova); bbm.1 to stain for β2m (kindly provided by G. Moldenhauer, German Cancer Research Center, Heidelberg, Germany); anti–HMB-45 (Dako) to detect melanoma cells; anti-CD3 (BD Pharmingen) to stain for T cells.
For flow cytometry, the mouse mAbs were as follows: anti–HLA-ABC-APC (eBiosciences), anti–CD54-PE and anti–HLA-DR-PECy7 (Beckmann Coulter), anti–PD-L1-PE and anti–PD-L2 (Biolegend), anti–B7-H3 (R&D Systems), anti–B7-H4 (eBiosciences); L243 was used for detection of HLA-DR molecules (17 (link)) and HC10 for labelling of β₂m-free HLA heavy chains (18 (link), 19 (link)).
The following antibodies were used for Western blot analysis: mouse anti–Melan-A/MART-1 (Zytomed), mouse anti-Tyrosinase and anti-MITF (Santa Cruz Biotechnology), rabbit anti-DCT/TRP2 (kindly provided by V. Hearing, National Cancer Institute, NIH, Bethesda); mouse anti-STAT1, rabbit anti-phospho(p)STAT1, rabbit anti-JAK1, rabbit anti-GAPDH (Cell Signaling Technology); rabbit anti-IRF1 and mouse anti-β2m (Santa Cruz Biotechnology); rabbit anti-β2m (Sigma); mouse anti-TAP1 (NOB-1) and mouse anti-tapasin (TO-3; ref. 20 (link)); mouse mAb HC10 (18 (link), 19 (link)).

Sucker A., Zhao F., Real B., Heeke C., Bielefeld N., Maβen S., Horn S., Moll I., Maltaner R., Horn P.A., Schilling B., Sabbatino F., Lennerz V., Kloor M., Ferrone S., Schadendorf D., Falk C.S., Griewank K, & Paschen A. (2014). Genetic Evolution of T-cell Resistance in the Course of Melanoma Progression. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(24), 6593-6604.

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Quantifying Murine Peritoneal Macrophages

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Peritoneal exudate cells (PECs) were obtained from naïve or infected mice by injecting 10 mL of ice-cold PBS. For flow cytometry, single cell suspensions of the PECs obtained during the sacrifice were stained with anti-F4/80, anti-PDL2, anti-IL-4Rα, anti-MGL1, and anti-MGL2 antibodies (Biolegend, San Diego, CA), for 30 min at 4°C. The cells were washed twice and analyzed using the FACSCalibur system and Cell Quest software (Becton Dickinson, USA).

Montero-Barrera D., Valderrama-Carvajal H., Terrazas C.A., Rojas-Hernández S., Ledesma-Soto Y., Vera-Arias L., Carrasco-Yépez M., Gómez-García L., Martínez-Saucedo D., Becerra-Díaz M, & Terrazas L.I. (2015). The Macrophage Galactose-Type Lectin-1 (MGL1) Recognizes Taenia crassiceps Antigens, Triggers Intracellular Signaling, and Is Critical for Resistance to This Infection. BioMed Research International, 2015, 615865.

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Modulation of Immune Ligand Expression

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Modulation of inhibitory and activating ligands was assessed using the following antibodies: mouse anti-MICA and MICB (R&D Systems), mouse anti–MHC class I (clone W6/32), anti-CD155 (R&D Systems) followed by secondary antibodies RPE-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories). PE-conjugated anti-TRAIL-R1, anti-TRAIL-R2, anti-CD95, anti-CD112 and FITC-conjugated anti-CD48 were purchased from Beckman Coulter. PE-conjugated anti PD-L1 and anti-PD-L2 were purchased from BioLegend. Data were analyzed using the FlowJo software (Flow Jo LLC).

Bommarito D., Martin A., Forcade E., Nastke M.D., Ritz J, & Bellucci R. (2016). Enhancement of tumor cell susceptibility to natural killer cell activity through inhibition of the PI3K signaling pathway. Cancer immunology, immunotherapy : CII, 65(3), 355-366.

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Profiling Macrophage Surface Markers

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BMDMs were suspended in fluorescence-activated cell sorting (FACS) buffer and incubated with Fc block (anti-mouse CD16/32; BioLegend). Cell surface markers were stained with anti-F4/80 (123108 or 123124; BioLegend), anti–PD-L1 (124308; BioLegend), anti–PD-L2 (107214; BioLegend), anti-Trem2 (FAB17291G; R&D) and, for live/dead staining, with LDFA (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit; L34957; Thermo Fisher Scientific). Afterward, the cells were washed, permeabilized with the Foxp3/Transcription Factor Staining Buffer Set (00-5523-00; Thermo Fisher Scientific), blocked with 2% normal mouse serum (Invitrogen), and stained for intracellular Arg1 (17-3697-82; Thermo Fisher Scientific). The cells were washed twice and then resuspended for analysis on a BD LSRFortessa FACS flow cytometer. Gating strategy is shown in fig. S4C. For live/dead staining, APC Annexin V Apoptosis Detection Kit with PI (640932; BioLegend) was used, and the cells were sorted on a BD FACSAria. Data analysis was carried out using FlowJo software.

Dichtl S., Lindenthal L., Zeitler L., Behnke K., Schlösser D., Strobl B., Scheller J., El Kasmi K.C, & Murray P.J. (2021). Lactate and IL6 define separable paths of inflammatory metabolic adaptation. Science Advances, 7(26), eabg3505.

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Multiparameter Flow Cytometry Profiling of Immune Cells

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Antibodies used for flow cytometry were Alexa Fluor488-anti-CD11b, PerCP-eF710/PE/or PE-Dazzel594-anti-HLA DR (clone L243), eF450-anti-HLA DR (clone LN3) PE/Cy7-anti-CD14, APC-anti-CD33, BV510-anti-CD3, BV605-anti-CD19, PerCP-Cy5.5-anti-CD66b, Alexa Fluor700 or PerCP-Cy5.5-anti-IFNγ, APC/Cy7-CD38, PerCP-Cy5.5-anti-CD69, PE-anti-CX3CR1, -anti-B7-H4, -anti-PD-L1, -anti-B7-H3, -anti-PD-L2, -anti-ICOS-L (all from Biolegend, San Diego, CA); FITC- or APCeF780-anti-CD4, Alexa Fluor700- or eF450-anti-CD25, PE-anti-FoxP3 and eF450-anti-IL10 (all from eBioscience); PE-anti- phospho-Zap70^(pY292), PE-anti-phospho-Akt^(pS473) from BD Biosciences. For neutralization studies, mAb to IL-10, IL-6 or rat IgG isotype (10 μg/mL, Biolegend) and polyclonal goat anti-IL-27 or normal goat IgG control (10 μg/mL R&D Systems) control were used.

Garg A., Trout R, & Spector S.A. (2017). Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4. Scientific Reports, 7, 44485.

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Flow Cytometry Analysis of Immune Cells

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Spleen cells and PECs were analyzed by FACS at 2 days and 8 weeks post-infection and from non-infected mice. Cells were analyzed for the expression of different markers and were previously gated from a high forward light scatter (FSC)/high side light scatter (SSC) gate. For the assay, 1 × 10⁶ cells were incubated with anti-CD16/CD32 antibody (Biolegend, San Diego, CA, USA) to block non-specific binding. Next, cells were stained with specific anti-F4/80, anti-PD-L1, anti-PD-L2, anti-MR, anti-CD11b, anti-Ly6C, anti-Ly6G (all from Biolegend), and anti-CCR2 (R&D Systems) and incubated for 30 min at 4 °C. Cells were washed twice with 1 mL of FACS buffer (1% FBS and 0.5% of sodium azide in PBS). Cell analysis was performed using the FACsAria system and FloJo software.

Becerra-Díaz M., Ledesma-Soto Y., Olguín J.E., Sánchez-Barrera A., Mendoza-Rodríguez M.G., Reyes S., Satoskar A.R, & Terrazas L.I. (2021). STAT1-Dependent Recruitment of Ly6ChiCCR2+ Inflammatory Monocytes and M2 Macrophages in a Helminth Infection. Pathogens, 10(10), 1287.

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