Total cholesterol (TChol), high-density lipoprotein (HDL)-cholesterol, triglycerides (TG), phosphatase alkaline (ALP) and the hepatic enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transpeptidase (γGT) were analyzed using commercial kits according to the manufacturer's instructions in a Hitachi 737 Automatic Analyzer (Hitachi Ltd., Tokyo, Japan) as were previously described (Warnick et al., 1990 (link); Crespillo et al., 2011 (link)).
737 automatic analyzer
Manufactured by Hitachi
Sourced in Japan
The Hitachi 737 Automatic Analyzer is a laboratory instrument designed for the automated analysis of various samples. It is capable of performing a range of clinical chemistry tests, including tests for enzymes, substrates, and electrolytes. The analyzer is equipped with advanced technology to ensure accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Rat insulin elisa kit, by Merck Group (1 mentions)
Rat insulin enzyme linked immunosorbent assay kit, by Mercodia (1 mentions)
Mouse il 6 elisa kit, by Merck Group (1 mentions)
Versamax, by Molecular Devices (1 mentions)
Rd291001200r, by BioVendor (1 mentions)
Rat insulin elisa kit, by BioVendor (1 mentions)
14 protocols using 737 automatic analyzer
1
Serum Lipid and Enzyme Analysis
2
Metabolic and Inflammatory Biomarkers in Plasma
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The following metabolites were measured in plasma: glucose, triglycerides, total cholesterol, high-density lipoprotein (HDL), bilirubin, urea, uric acid, creatinine, and the hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST). They were analyzed using commercial kits, according to the manufacturer’s instructions, in a Hitachi 737 Automatic Analyzer (Hitachi, Tokyo, Japan). Very-low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) were determined through use of modified Friedewald equations: VLDL = triglycerides/5; LDL = cholesterol – ((triglycerides/5) + HDL) [27 (link)]. The plasma levels of insulin and interleukin 6 (IL-6) were measured using a commercial rat insulin enzyme-linked immunosorbent assay (ELISA) kit (EZRMI-13K Millipore, Missouri, USA), a Rat IL-6 ELISA Kit (KRC006 Novex, by Life technologies, USA), and a tumor necrosis factor alpha (TNF-α) Kit (KRC3011 Novex, by Life technologies, USA). Homeostatic Model Assessment for Insulin Resistance (HOMA IR) was calculated using the following formula: HOMA IR = (fasting plasma insulin (uIU/mL) × fasting glycemia (mmol/L)/22.5) [28 (link)].
3
Metabolic Profiling of Rat Plasma
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The following metabolites were measured in plasma: glucose, triglycerides, total cholesterol, HDL, bilirubin, urea, uric acid, creatinine, and the hepatic enzymes GPT and GOT. They were analyzed using commercial kits according to the manufacturer’s instruction in a Hitachi 737 Automatic Analyzer (Hitachi, Tokyo, Japan). VLDL and LDL were determined via the modification of Friedewald equation78: VLDL = TG/5; LDL = [(TChol/1.19) + (TG/1.9) − (HDL/1.1) − 38]. The plasma levels of insulin, Interleukin 6 (Il-6), and Tumor necrosis factor alpha (Tnf-α) were measured using the following commercial rat kits: rat insulin ELISA kit (EZRMI-13K Millipore, St. Louis, MO, USA), Rat Il-6 ELISA Kit (KRC006 Novex, by Life technologies, Carlsbad, CA, USA), and Tnf-α Kit (KRC3011 Novex, by Life technologies, USA).
4
Biochemical Markers in Hepatic Assessment
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The hepatic enzymes gamma-glutamyl transpeptidase (γGT), glutamate pyruvate transaminase (GPT) and glutamic oxaloacetic transaminase (GOT), and creatinine and bilirubin were analyzed using commercial kits according to the manufacturer's instructions in a Hitachi 737 Automatic Analyzer (Hitachi Ltd., Tokyo, Japan). A calibration curve and internal controls were included in each assay.
5
Plasma Metabolite Profiling Assay
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The following metabolites were measured in the plasma samples: glucose, triglycerides, cholesterol, high-density lipoprotein cholesterol (HDL-C), uric acid, urea, creatinine, glutamate-oxoloacetate transaminase (GOT), glutamate-pyruvate transaminase (GPT) and ɣ-glutamyl transpeptidase (GGt). These metabolites were analyzed using commercial kits according to the manufacturer’s instruction in a Hitachi 737 Automatic Analyzer (Hitachi, Tokyo, Japan).
6
Metabolic Profiling in Plasma
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The following metabolites, metabolic hormones and adipokines were measured in plasma: uric acid, urea, creatinine, aspartate transaminase (AST), alanine transaminase (ALT), γ-glutamyl transpeptidase (GGt), leptin, interleukin-6, tumor necrosis factor α (TNF-α), glucose, insulin, cholesterol, high-density lipoprotein cholesterol and triglycerides.
The metabolites were analyzed using commercial kits according to the manufacturer's instructions in a Hitachi 737 Automatic Analyzer (Hitachi, Tokyo, Japan). The levels of leptin, IL-6 and TNF-α were measured using commercial rat enzyme-linked immunosorbent assay kits (Abcam, Cambridge, UK). The plasma levels of insulin were measured using a commercial rat insulin enzyme-linked immunosorbent assay kit (Mercodia, Uppsala, Sweden).
The metabolites were analyzed using commercial kits according to the manufacturer's instructions in a Hitachi 737 Automatic Analyzer (Hitachi, Tokyo, Japan). The levels of leptin, IL-6 and TNF-α were measured using commercial rat enzyme-linked immunosorbent assay kits (Abcam, Cambridge, UK). The plasma levels of insulin were measured using a commercial rat insulin enzyme-linked immunosorbent assay kit (Mercodia, Uppsala, Sweden).
7
Quantitative Analysis of Cytokine and Lipid Levels
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The mice were sacrificed at 1 h after the last inoculation with rIL-6 or vehicle. Blood samples were collected in vacutainer tubes and incubated at room temperature for 1 h for clotting. After centrifugation the sera were extracted, aliquoted and stored at −80°C. The IL-6 concentrations in the sera were assayed in duplicate using the Mouse IL-6 ELISA kit (Millipore, Temecula, CA, USA) according to the manufacturer's instructions. The absorbance was measured at 450 nm using an ELISA microplate reader (VERSAmax; Molecular Devices, Sunnyvale, CA, USA), and the cytokine concentrations were calculated based on the optical densities obtained with the standards. The levels of triglycerides (TG), total cholesterol and high-density lipoprotein (HDL) cholesterol were analyzed using a Hitachi 737 Automatic Analyzer (Hitachi Ltd, Tokyo, Japan).
8
Hepatic Enzyme Assay Protocol
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ALP and the hepatic enzymes ALT or GPT, AST or GOT, and γGT were analyzed using commercial kits according to the manufacturer’s instructions in a Hitachi 737 Automatic Analyzer (Hitachi Ltd., Tokyo, Japan). In all cases, a calibration curve and internal controls were included in each assay.
9
Comprehensive Metabolic Profiling in Rats
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The following plasma metabolites and enzymes were measured: basal glucose, triglycerides, total cholesterol, high-density lipoprotein (HDL), urea, bilirubin, alkaline phosphatase (ALKP), and the hepatic enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transpeptidase (γGT). These metabolites were analyzed using commercial kits according to the manufacturer's instructions and a Hitachi 737 Automatic Analyzer (Hitachi Ltd, Tokyo, Japan). Very low-density lipoprotein (VLDL) were estimated following the Friedewald equation (Warnick et al., 1990 (link)) and low-density lipoprotein (LDL) was determined by the modification of Friedewal equation proposed by Ahmadi et al. (2008 (link)): VLDL = TG/5; LDL = [(TChol/1.19)+(TG/1.9)−(HDL/1.1)−38]. The plasma levels of leptin were measured using a commercial rat leptin ELISA kit (Cat. no. RD291001200R; BioVendor, Brno, Czech Republic).
10
Plasma Metabolite Profiling in Preclinical Studies
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The following plasma metabolites were measured: basal glucose, triglycerides, total cholesterol, high-density lipoprotein (HDL), urea, bilirubin, alkaline phosphatase (ALKP), and the hepatic enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyltranspeptidase (γGT). These metabolites were analyzed using commercial kits according to the manufacturer’s instructions and a Hitachi 737 Automatic Analyzer (Hitachi Ltd, Tokyo, Japan). Very low-density lipoprotein (VLDL) was estimated following the Friedewald equations [46 (link)] and low-density lipoprotein (LDL) was determined by the modification of Friedewal equation proposed by Ahmadi et al. [47 (link)]: (VLDL = TG/5); LDL = [(TChol/1.19)+(TG/1.9)- (HDL/1.1)-38]. The leptin levels in plasma were measured using a commercial rat insulin ELISA kit (Cat. no. RD291001200R; BioVendor, Brno, Czech Republic).
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