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Anti cd45ra bv605

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Anti-CD45RA-BV605 is a flow cytometry reagent that binds to the CD45RA surface antigen and is conjugated to the BV605 fluorophore. It is used to identify and analyze T cell subsets in flow cytometry applications.

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Lab products found in correlation

Bv510 streptavidin, by BioLegend (2 mentions) Anti cd8 fitc clone sk1, by BioLegend (2 mentions) Anti granzymeb pacific blue, by BioLegend (2 mentions) Anti cd62l pe cy5 clone dreg 56, by BioLegend (2 mentions) Anti cd3 af700 clonehit3a, by BioLegend (2 mentions) Anti granzyme a percp cy5, by BioLegend (2 mentions) Bv650 streptavidin, by R&D Systems (2 mentions) Anti granzyme k pe, by BioLegend (2 mentions) Anti granzyme m efluor660, by Thermo Fisher Scientific (2 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Fixation permeabilization solution kit, by BD (1 mentions) Anti cd8 percp cy5, by BioLegend (1 mentions) Anti pd 1 percpcy 5, by BioLegend (1 mentions) Anti ccr7 pe, by BioLegend (1 mentions) Anti cd86 apc, by BioLegend (1 mentions) Anti cd19 bv605, by BioLegend (1 mentions) Anti cd69 fitc, by BD (1 mentions) Anti cd4 pe cy7, by BioLegend (1 mentions) Anti hla dr fitc, by BD (1 mentions) Anti pd l1 bv421, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-CD45RA (16 citations) CD45RA BV605 (10 citations)

4 protocols using anti cd45ra bv605

1

Assessing CD8+ T Cell Cytolytic Potential

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Rested ex vivo CD8+ T cells were assessed for CMV and
HIV specificity using HLA-matched tetramers. Tetramers were made via
biotinylated monomer conjugation to BV510 streptavidin (BioLegend). Monomers
were obtained from Dr. Soren Buus (University of Copenhagen). The A02-NV9
tetramer was used to stain for CMV-specificity while pools of A02-SL9, B27-KK10,
B53-QW9, B53-YY9, B57-IW9(p24), B57-IW9(RT), B57-KF11, B57-QW9, and B57-TW10
tetramers were used to stain for HIV-specificity. Subsequent surface stains
included anti-CD62L-PE-Cy5 (clone DREG-56; BioLegend), anti-CD3-AF700 (clone
HIT3a; BioLegend), anti-CD8-FITC (clone SK1; BioLegend), anti-CD45RA-BV605
(clone HI100; BioLegend), and LIVE/DEAD Blue. Following permeabilization, the
cells were intracellular stained using anti-perforin PE/Cy7 (clone B-D48;
BioLegend), anti-granzyme A-PerCp/Cy5.5 (clone CB9; BioLegend), anti-granzyme
B-Pacific Blue (clone GB11; BioLegend), anti-granzyme H (biotinylated and
secondary stained with BV650 streptavidin) (polyclonal antibody, R&D),
anti-granzyme K-PE (clone GM26E7; BioLegend), and anti-granzyme M-eFluor660
(clone 4B2G4; eBioscience). The cells were analyzed by flow cytometry.
Naïve CD8+ T cells within the mixed population of
CD8+ T cells were used as internal gating controls for
perforin and each granzyme as these cells do not express these effector
molecules.
Clayton K.L., Collins D.R., Lengieza J., Ghebremichael M., Dotiwala F., Lieberman J, & Walker B.D. (2018). Resistance of HIV-infected macrophages to CD8 T lymphocyte-mediated killing drives immune activation. Nature immunology, 19(5), 475-486.
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2

T Cell Phenotyping by Flow Cytometry

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T cells were phenotyped by flow cytometry staining before (Day 0 – naïve and Day 10–10 days after 1st round of stimulation) and after restimulation (Day 15), as well as in co-cultures with tumor cells. Briefly, CD8+ T cells were stained with LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit (Thermo Scientific), followed by surface staining at 4 ⁰C with: – anti-CD8 AF647, anti-CD8 PerCPCy5.5, anti-CD45RO Alexa Fluor 488, anti-CD45RA BV605, anti-CCR7-PE, anti-PD-1 PerCpCy5.5 (all from Biolegend) antibodies. Staining of CCR7 was performed at 37 ⁰C for 30 min. Intracellular granzyme B staining was performed with anti-Granzyme B-AF647 antibody (Biolegend), after permeabilization with Fixation/Permeabilization Solution Kit (BD Biosciences). Flow cytometric analysis was performed using a BD Fortessa. Data were analyzed using FlowJo (Tree Star).
Nelson N., Lopez-Pelaez M., Palazon A., Poon E., De La Roche M., Barry S., Valge-Archer V., Wilkinson R.W., Dovedi S.J, & Smith P.D. (2019). A cell-engineered system to assess tumor cell sensitivity to CD8+ T cell-mediated cytotoxicity. Oncoimmunology, 8(8), 1599635.
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3

Comprehensive Immunophenotyping of Immune Cells

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Anti-CD19 APC-Cy7, anti-CD38 PerCp-Cy5.5, anti-CD4 PE-Cy7, anti-CD25 PerCP-Cy5.5, anti-CD86 APC, anti-CD80 BV421, anti-CD45RA BV605, anti-ICOS PE, anti-CD21 PE-Cy7, anti-CD80 BV421, anti-CD27 PerCp-Cy5.5, anti-PD-1 APC, anti-CD45RA APC-Cy7, anti-HLA-DR BV605, anti-CD11c AF700, anti-CD86 BV711, anti-CD95 BV650, anti-IgD BV786, anti-FCRL5 APC, anti-CXCR3 BV421, anti-CCR6 BV605, anti-CD19 BV650, anti-CD19 BV605, anti-PD-L1 BV421, anti-PD-L2 PE, anti-ICOS-L PE, anti-OX40L PE, anti-IL-10 PE, anti-IL-10 APC, anti-TNF-α APC-Cy7 (all obtained from BioLegend); anti-IgD FITC and anti-IgD PE (both from Southern Biotech); anti-CD27 PE (Dako); and anti-CD21 PE-Cy7, anti-CD69 FITC, anti-HLA-DR FITC, anti-CD27 BV605, anti-CD40 FITC, anti-CD40L PE, anti-ICAM-1 PE, anti-CXCR5 BUV395 and anti-CD27 BUV395 (all from BD Biosciences).
Reincke M.E., Payne K.J., Harder I., Strohmeier V., Voll R.E., Warnatz K, & Keller B. (2020). The Antigen Presenting Potential of CD21low B Cells. Frontiers in Immunology, 11, 535784.
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4

Assessing CD8+ T Cell Cytolytic Potential

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rested ex vivo CD8+ T cells were assessed for CMV and
HIV specificity using HLA-matched tetramers. Tetramers were made via
biotinylated monomer conjugation to BV510 streptavidin (BioLegend). Monomers
were obtained from Dr. Soren Buus (University of Copenhagen). The A02-NV9
tetramer was used to stain for CMV-specificity while pools of A02-SL9, B27-KK10,
B53-QW9, B53-YY9, B57-IW9(p24), B57-IW9(RT), B57-KF11, B57-QW9, and B57-TW10
tetramers were used to stain for HIV-specificity. Subsequent surface stains
included anti-CD62L-PE-Cy5 (clone DREG-56; BioLegend), anti-CD3-AF700 (clone
HIT3a; BioLegend), anti-CD8-FITC (clone SK1; BioLegend), anti-CD45RA-BV605
(clone HI100; BioLegend), and LIVE/DEAD Blue. Following permeabilization, the
cells were intracellular stained using anti-perforin PE/Cy7 (clone B-D48;
BioLegend), anti-granzyme A-PerCp/Cy5.5 (clone CB9; BioLegend), anti-granzyme
B-Pacific Blue (clone GB11; BioLegend), anti-granzyme H (biotinylated and
secondary stained with BV650 streptavidin) (polyclonal antibody, R&D),
anti-granzyme K-PE (clone GM26E7; BioLegend), and anti-granzyme M-eFluor660
(clone 4B2G4; eBioscience). The cells were analyzed by flow cytometry.
Naïve CD8+ T cells within the mixed population of
CD8+ T cells were used as internal gating controls for
perforin and each granzyme as these cells do not express these effector
molecules.
Clayton K.L., Collins D.R., Lengieza J., Ghebremichael M., Dotiwala F., Lieberman J, & Walker B.D. (2018). Resistance of HIV-infected macrophages to CD8 T lymphocyte-mediated killing drives immune activation. Nature immunology, 19(5), 475-486.
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