Irdye800 anti rabbit igg

Manufactured by LI COR

Sourced in United States

The IRDye800 anti-rabbit IgG is a fluorescent secondary antibody used for detection in Western blotting, immunohistochemistry, and other immunoassay applications. It is designed to specifically bind to rabbit primary antibodies, allowing for the visualization and quantification of target proteins.

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Lab products found in correlation

Anti gef h1, by Cell Signaling Technology (1 mentions) Anti rock1, by Cell Signaling Technology (1 mentions) Anti jnk2, by Cell Signaling Technology (1 mentions) Alexa 594 anti mouse igg, by Thermo Fisher Scientific (1 mentions) Anti actin, by Merck Group (1 mentions) Rabbit anti pericentrin, by Abcam (1 mentions) Alexa 488 anti chicken igg, by Thermo Fisher Scientific (1 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Odyssey infrared image system, by LI COR (1 mentions) Tom20, by Abcam (1 mentions) Gapdh, by Proteintech (1 mentions)

Close spelling variants of this product

IRDye800-conjugated goat anti-rabbit and anti-mouse IgG IRDye680LT- and IRDye800-conjugated anti-rabbit and anti-mouse IgG secondary antibodies IRDye800-conjugated anti-rabbit IgG (926-32211) IRDye800 conjugated anti-rabbit IgG IRdye800-conjugated donkey anti-rabbit and donkey anti-mouse IgG

4 protocols using irdye800 anti rabbit igg

Antibody Validation for ASAP1 and Related Proteins

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A monoclonal mouse anti-ASAP1 antibody was purchased from Abnova. A polyclonal rabbit anti-ASAP1 serum was raised in our lab as previously described (31 (link)). Anti-paxillin and anti-GM130 antibodies were purchased from BD Biosciences, anti-ROCK2 antibody from BD Transduction Laboratories, anti-HABP4 antibody from Bioss USA, anti-Akt, anti-Arf6, anti-GEF-H1, anti-JNK2, anti-PRKAR1A (PKA RI-α/β), anti-ROCK1, anti-SMARCA5 (SNF2H), anti-TIAM1, and anti-VAV2 from Cell Signaling Technology, anti-β-COP antibody from Invitrogen, IRDye680 anti-mouse IgG and IRDye800 anti-rabbit IgG were from LI-COR, and anti-Brag2 (IqSec1) antibody was from Sigma Precision Antibodies.

Rosenberg EM J.r., Jian X., Soubias O., Yoon H.Y., Yadav M.P., Hammoudeh S., Pallikkuth S., Akpan I., Chen P.W., Maity T.K., Jenkins L.M., Yohe M.E., Byrd R.A, & Randazzo P.A. (2023). The small molecule inhibitor NAV-2729 has a complex target profile including multiple ADP-ribosylation factor regulatory proteins. The Journal of Biological Chemistry, 299(3), 102992.

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Zika Virus Antibody Detection Assay

Cited 1 time

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The following antibodies were used in this study: mouse anti-ZIKV NS5 (BioFront Technologies, BF-6A), chicken anti-ZIKV NS5 (generously provided by Dr. Sonja Best, National Institute of Allergy and Infectious Diseases) (Grant et al., 2016 (link)), rabbit anti-GFP (Invitrogen, A-6455), anti-actin (Sigma Aldrich, A1978), rabbit anti-pericentrin (Abcam, ab4448), Alexa-594 anti-mouse IgG (Invitrogen, A11032), Alexa-488 anti-chicken IgG (Invitrogen, A11039), Alexa-488 anti-rabbit IgG (Invitogen, A11034), Alexa-594 anti-rabbit IgG (Invitogen, A11012), IRDye800 anti-rabbit IgG (LI-COR, 926-32211), IRDye800 anti-chicken IgG (LI-COR, 92532218), and IRDye 680 anti-mouse IgG (LI-COR, 925-68070).

Kesari A.S., Heintz V.J., Poudyal S., Miller A.S., Kuhn R.J, & LaCount D.J. (2019). Zika virus NS5 localizes at centrosomes during cell division. Virology, 541, 52-62.

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Near-Infrared Protein Detection Protocol

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The general western protocol using a near-infrared detection method was described previously with slight modification [34 (link)]. Wet transfer was performed using the Bio-Rad transfer kit at 300 mA for 120 min at 4 °C. Secondary antibody incubation (LI-COR IRDye 680 donkey anti-mouse or anti-goat IgG or IRDye 800 anti-rabbit IgG, 1:10,000) was performed in the blocking buffer for 2 h at room temperature. A LI-COR Odyssey imaging system (Lincoln, NE) was used for analysis.

Xie J., Huang X., Park M.S., Pham H.M, & Chan W.K. (2014). Differential suppression of the aryl hydrocarbon receptor nuclear translocator-dependent function by an aryl hydrocarbon receptor PAS-A-derived inhibitory molecule. Biochemical pharmacology, 88(2), 253-265.

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NDRG4 Protein Analysis by Western Blot

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Western blot analyses were performed as described previously (29 (link)). Primary antibodies used were those against NDRG4 (1 : 1,000; Proteintech), Tom20 (1 : 1,000; Abcam), and GAPDH (1 : 5,000, Proteintech). After incubating with IRDye 680 anti-mouse IgG (LI-COR, Lincoln, NE) and IRDye 800 anti-rabbit IgG (LI-COR, Lincoln, NE) secondary antibodies, signal intensities were analyzed using an Odyssey Infrared Image System (LI-COR Bio-sciences).

Shi H.H., Liu H.E, & Luo X.J. (2020). Hypermethylation-mediated silencing of NDRG4 promotes pancreatic ductal adenocarcinoma by regulating mitochondrial function. BMB Reports, 53(12), 658-663.

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