4 nitrophthalonitrile

Manufactured by Merck Group

4-nitrophthalonitrile is a chemical compound used as a laboratory reagent. It is a crystalline solid with the molecular formula C8H3N3O2.

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2 protocols using 4 nitrophthalonitrile

Synthesis and Characterization of Functional PEG

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Phosphate-buffered saline (PBS, 10× concentrate) and formaldehyde (for molecular biology, 36.5–38% in H₂O) were purchased from (Sigma Aldrich, St. Louis, MI, USA). Human colon cancer cells (CaCo-2 Cellonex Cat SS1402 CCAC-FL; CCAC-C) were purchased from the (American Type Culture Collection, Manassas, VA, USA). Tetrahydrofuran (THF), 1,8-diazabicyclo [5.4.0] undec-7-ene (DBU), aluminum chloride, dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), sodium 2-mercaptoacetate, deuterated water (D₂O), 4-nitrophthalonitrile, dichloromethane (DCM), methanol, 1-pentanol, N-hydroxysuccinimide (NHS), amino-polyethylene glycol-thiol (NH₂-PEG(2000)-SH), and N,N′-dicyclohexylcarbodiimide (DCC) were purchased from Sigma–Aldrich. Sodium 2-mercaptoacetate phthalonitrile was synthesized as previously reported [52 (link)].

Simelane N.W., Matlou G.G, & Abrahamse H. (2023). Photodynamic Therapy of Aluminum Phthalocyanine Tetra Sodium 2-Mercaptoacetate Linked to PEGylated Copper–Gold Bimetallic Nanoparticles on Colon Cancer Cells. International Journal of Molecular Sciences, 24(3), 1902.

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Electrochemical Characterization of Organic Compounds

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Phenolphthalein (C₂₀H₁₄O₄, 98%), methanol (CH₃OH, 99.8%), ethanol (C₂H₅OH, 99.5%), acetone
(CH₃COCH₃, 99.5%), 4-nitrophthalonitrile (C₈H₃N₃O₂, 99.0%), potassium
carbonate (K₂CO₃, 99.0%), cobalt(II) chloride
hexahydrate (CoCl₂•6H₂O, 98%), amyl alcohol
(C₅H₁₂O, 99%), DBU (C₉H₁₆N₂, 98%), n-hexane (C₆H₁₄, 95%), ethyl acetate (C₄H₈O₂, 99.8%), dimethyl formamide (DMF: C₃H₇NO,
99.8%), dimethyl sulfoxide (DMSO: C₂H₆OS, 99.9%),
tetrabutylammonium perchlorate [TBAP: (C₄H₉)₄N(ClO₄), 99.0%], hydrochloric acid (HCl, 37%),
sulfuric acid (H₂SO₄, 97%), isopropyl alcohol
(IPA, 99.0%), and potassium ferricyanide (K₄[Fe(CN)₆], 97%) were purchased from Sigma-Aldrich, Pt/C (20%) was
purchased from Thermo Fisher Scientific, and MWCNTs were purchased
from AkzoNobel. Other chemical reagents used to prepare solutions
were obtained from Merck. Double-distilled water was used throughout
the experiments.

G.i.d.d.a.e.r.a.p.p.a., Manjunatha N., S.h.a.n.t.h.a.r.a.j.a., Hojamberdiev M, & Sannegowda L.K. (2022). Tetraphenolphthalein Cobalt(II) Phthalocyanine Polymer Modified with Multiwalled Carbon Nanotubes as an Efficient Catalyst for the Oxygen Reduction Reaction. ACS Omega, 7(16), 14291-14304.

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