Peroxidase conjugated anti mouse igg nxa931

Dulbecco’s modified Eagle’s medium (11960-077), sodium pyruvate (11360-070), L-Glutamine (25030-164) and 0.25% trypsin-EDTA (25200-056) were purchased from Gibco (Grand Island, NY). Heat-inactivated fetal calf serum (FCS, F4135), anti-β-actin (A5441) and A23187 ionophore (C7522), trypan blue (T8154) were purchased from Sigma (St. Louis, MO). Cell lysis Buffer (9803) was purchased from Cell Signaling Technology (Danvers, MA). Anti-TG2 (MAB3839) was purchased from EMD Milipore (Bedford, MA). Antibody for Sox2 (ab15830-100) was purchased from Abcam. Antibodies for caspase-3 (9665) and Nanog (4839), and NFκB-p65 siRNA (6261) were purchased from Cell Signaling Technologies. Anti-Oct4 (611203) was purchased from BD Transduction Laboratories (San Jose, CA). Peroxidase-conjugated anti-mouse IgG (NXA931) and anti-rabbit IgG (NA934V) were obtained from GE Healthcare (Buckinghamshire, UK). Production of NC9 was described previously (16 ). TG2- (sc-37514) and control-siRNA (sc-37007) were purchased from Santa Cruz (Dallas, TX). Anti-TG1 (SC-166467) and anti-NFκB-p65 (sc-109) were purchased from Santa Cruz (Dallas, TX). Anti-FXIIIa (ab79759) was purchased from Abcam. Fluorescein cadaverine (FC) was purchased from Life Technologies. BD Biocoat cell inserts (353097) and Matrigel (354234) were purchased from BD Biosciences. Proteins were detected by immunoblot (17 (link), 18 (link)).