Skin samples were fixed in 4% paraformaldehyde,dehydrated in 30% sucrose, embedded in OCT and frozen. Skin cryostat sections (5mm) were stained with antibodies specific for YFP (life technologies, Grand Island, NY), OX40 (BD Biosciences), filaggrin (Santacruz, Dallas, TX), involucrin (Covance, Princeton, NJ), loricrin, cytokeratin14, cytokeratin15, cytokeratin10 (Genetex, Irvine, CA) Ki67, CD4 (eBioscience), cleaved caspase 3 (Cellsignaling), and TSLP (R&D Systems, Minneapolis, MN), then stained with fluorescently-coupled secondary anti-rabbit or -rat IgG (Cellsignaling Technologies, Danvers, MA), or streptavidin-conjugated Cy5 (Biolegend). Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany).
Fluorescently coupled secondary anti rabbit or rat igg
Manufactured by Cell Signaling Technology
Fluorescently-coupled secondary anti-rabbit or -rat IgG is a laboratory product used for the detection and visualization of target proteins in various experimental techniques, such as Western blotting and immunofluorescence. The product consists of secondary antibodies that are conjugated with fluorescent dyes, allowing for the indirect detection of primary antibodies raised against rabbit or rat antigens.
Automatically generated - may contain errors
Lab products found in correlation
Filaggrin, by Santa Cruz Biotechnology (2 mentions)
Loricrin, by GeneTex (2 mentions)
Cytokeratin14, by GeneTex (2 mentions)
Cytokeratin15, by GeneTex (2 mentions)
Cytokeratin10, by GeneTex (2 mentions)
Streptavidin conjugated cy5, by BioLegend (2 mentions)
Leica dmi6000b fluorescence microscope, by Leica Microsystems (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Involucrin, by Fortrea (2 mentions)
2 protocols using fluorescently coupled secondary anti rabbit or rat igg
1
Immunostaining of Skin Cryosections
2
Immunostaining of Skin Cryosections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Skin samples were fixed in 4% paraformaldehyde,dehydrated in 30% sucrose, embedded in OCT and frozen. Skin cryostat sections (5mm) were stained with antibodies specific for YFP (life technologies, Grand Island, NY), OX40 (BD Biosciences), filaggrin (Santacruz, Dallas, TX), involucrin (Covance, Princeton, NJ), loricrin, cytokeratin14, cytokeratin15, cytokeratin10 (Genetex, Irvine, CA) Ki67, CD4 (eBioscience), cleaved caspase 3 (Cellsignaling), and TSLP (R&D Systems, Minneapolis, MN), then stained with fluorescently-coupled secondary anti-rabbit or -rat IgG (Cellsignaling Technologies, Danvers, MA), or streptavidin-conjugated Cy5 (Biolegend). Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!