An Agilent 6530 Accurate-Mass QTOF-MS system was connected with HPLC system via an Agilent Jet Stream electrospray (ESI) interface and an Agilent 1260 HPLC with diode array detector (DAD) (Agilent Technologies, Santa Clara, CA, USA). Kq-100e Ultrasonic cleaning instrument (KQ-250DB, Kunshan Ultrasonic Instruments Co., Ltd., Kunshan, China) was used.
Jet stream electrospray
Manufactured by Agilent Technologies
Sourced in United States
The Jet Stream electrospray is a type of ion source used in mass spectrometry. It generates charged droplets of a sample solution, which are then vaporized and ionized, allowing the analytes to be detected and analyzed by the mass spectrometer.
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Lab products found in correlation
3 protocols using jet stream electrospray
1
HPLC-QTOF-MS Analytical Protocol
2
Phytochemical Profiling by UHPLC-QTOF-MS
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Phytochemical analysis ASE was assessed using Ultra High-performance liquid chromatography Photodiode-Array Detector 323 Mass Spectrophotometer (HR-LCMS 1290 Infinity UHPLC System), Agilent 324 Technologies®, USA. The liquid chromatographic system consisted of a HiP sampler, binary gradient solvent pump, column compartment and Quadrupole Time of Flight Mass Spectrometer (MS Q-TOF) with a dual Agilent Jet Stream Electrospray (AJS ES) ion source. 10 μL of sample was injected into the system, followed by separation in SB-C18 column (2.1 × 50 mm, 1.8 μm particle size). 1% formic acid in deionized water (solvent A) and acetonitrile (solvent B) were used as solvents. Flow rate of 0.350 mL/min was used, while MS detection was performed in MS Q-TOF. Compounds were identified via their mass spectra and their unique mass fragmentation patterns. Compound Discoverer 2.1, ChemSpider and PubChem were used as the main tools for the identification of the phytochemical constituents.
3
Quantitative Analysis of Ranolazine and Metabolites
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The samples were analyzed using a UHPLC-MS-MS instrument constituted by a model 1290 ultra high performance liquid chromatograpy (UHPLC) (Agilent, Palo Alto, CA) coupled to a model 6460 triple quadruple mass spectrometer (Agilent) through a Jetstream Electrospray (ESI) source (Agilent) operating in positive ionization mode. A UHPLC Zorbax Eclipse reversed phase column (RRHD 2.1 × 100 mm, 1.8 µm) (Agilent) was used with a gradient elution program. The elution profile was as follows. Eluent A: 5 mM aqueous ammonium formate added with 0.01% formic acid; eluent B: methanol/acetonitrile (90/10, v/v) containing 0.01% formic acid; gradient: 0-4 min from 40 to 60% B, 4-5 min from 60 to 95% B, hold for 2 min at 95% B, before re-equilibration with 60% A and 60% B. Under the described conditions, ranolazine and its metabolite were baseline separated. The following ESI conditions were applied: drying gas, nitrogen (8 L/min, 320 • C); nebulizer gas, nitrogen (30 psi); sheath gas, nitrogen (
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