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Anti α smooth muscle actin

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Anti-α-smooth muscle actin (SMA) is a monoclonal antibody that binds to α-smooth muscle actin, a cytoskeletal protein expressed in vascular smooth muscle cells. This antibody can be used to detect and identify smooth muscle cells in various tissue samples.

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Lab products found in correlation

Anti e cadherin, by R&D Systems (2 mentions) Anti nf κb p65 subunit antibody, by Abcam (2 mentions) Anti mcp 1, by Abcam (2 mentions)

Spelling variants of this product

Anti-actin (3 citations) α-smooth muscle actin (2 citations) α-actin (2 citations) PE-conjugated anti-mouse α-smooth muscle actin (α-SMA) (2 citations) Anti-α-smooth muscle actin (α-SMA) antibody (2 citations)

2 protocols using anti α smooth muscle actin

1

Protein Expression Analysis in Cell Types

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell protein and nuclear protein preparation, as well as western blotting, were performed as we described previously [24 (link), 25 (link)]. For whole protein, the membrane was probed with primary antibodies of anti-E-cadherin (rabbit polyclonal, R&D System, 1:1000), anti-α-smooth muscle actin (SMA) (rabbit polyclonal, R&D System, 1:1000), anti-fibroblast specific protein (FSP)-1 (rabbit polyclonal, Abcam, 1:1000), anti-collagen I (rabbit polyclonal, Calbiochem, 1:1000) and anti-MCP-1 (rabbit polyclonal, Abcam, 1:500); for nuclear protein, the membrane was probed with anti-NF-κB-p65 subunit antibody (rabbit polyclonal, Abcam, 1:1000). The intensities of the blots were determined using an imaging analysis program (Image J, free download from http://rsbweb.nih.gov/ij/). The β-actin was used as internal control. The normalized values in different groups were averaged and expressed as fold change with the mean value of control group as 1.
Hu J., Zhu Q., Li P.L., Wang W., Yi F, & Li N. (2015). Stem Cell Conditioned Culture Media Attenuated Albumin-Induced Epithelial– Mesenchymal Transition in Renal Tubular Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 35(5), 1719-1728.
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2

Protein Expression Analysis in Cell Types

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell protein and nuclear protein preparation, as well as western blotting, were performed as we described previously [24 (link), 25 (link)]. For whole protein, the membrane was probed with primary antibodies of anti-E-cadherin (rabbit polyclonal, R&D System, 1:1000), anti-α-smooth muscle actin (SMA) (rabbit polyclonal, R&D System, 1:1000), anti-fibroblast specific protein (FSP)-1 (rabbit polyclonal, Abcam, 1:1000), anti-collagen I (rabbit polyclonal, Calbiochem, 1:1000) and anti-MCP-1 (rabbit polyclonal, Abcam, 1:500); for nuclear protein, the membrane was probed with anti-NF-κB-p65 subunit antibody (rabbit polyclonal, Abcam, 1:1000). The intensities of the blots were determined using an imaging analysis program (Image J, free download from http://rsbweb.nih.gov/ij/). The β-actin was used as internal control. The normalized values in different groups were averaged and expressed as fold change with the mean value of control group as 1.
Hu J., Zhu Q., Li P.L., Wang W., Yi F, & Li N. (2015). Stem Cell Conditioned Culture Media Attenuated Albumin-Induced Epithelial– Mesenchymal Transition in Renal Tubular Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 35(5), 1719-1728.
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