To visualize the skeleton, the chick embryos were stained with alcian blue and alizarin red as previously described (Schmitz et al., 2010a (link)). Day-17 chick embryos were freed from adherent tissue, fixed in 95% ethanol for 3 days, stained for cartilage with alcian blue and counterstained for bone with alizarin red (Solarbio, Beijing, China). Long-bone tissues were carefully photographed using a stereomicroscope (Olympus MVX10, Japan). The length of the alizarin red-stained portion of each radius, ulna, tibia and phalanx was quantified using Image Pro-Plus 5.0 (Media Cybernetics).
Alizarin red
Manufactured by Media Cybernetics
Sourced in United States
Alizarin Red is a histological stain used to detect the presence of calcium in biological samples. It binds to calcium ions, producing a red-colored complex that can be visualized under a microscope. The staining procedure is commonly used in various fields, including medical research and pathology, to analyze the mineralization of tissues.
Automatically generated - may contain errors
Lab products found in correlation
Alizarin red, by Solarbio (2 mentions)
Mvx10, by Olympus (1 mentions)
Image pro plus 5, by Media Cybernetics (1 mentions)
Image pro plus 6, by Media Cybernetics (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Alizarin red, by Merck Group (1 mentions)
Osteogenic medium, by Thermo Fisher Scientific (1 mentions)
Image pro plus software, by Media Cybernetics (1 mentions)
Ix71 f22fl dic optical microscope, by Olympus (1 mentions)
3 protocols using alizarin red
1
Chick Embryo Skeletal Visualization
2
Evaluating Osteogenic Differentiation of BMSCs
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To evaluate the osteogenic differentiation potential, Lon-BMSCs and Al-BMSCs or
Lon-BMSCs and Al-BMSCs sheets were incubated in the osteogenic medium
(Invitrogen). The medium was changed every 2 days. On day 5 after induction,
cells or cell sheets were fixed with 4% paraformaldehyde for alkaline
phosphatase (ALP) staining following the manufacturer’s protocol
(Sigma-Aldrich). On day 14 after induced for osteogenic induction, cells or cell
sheets were fixed with 70% ethanol, and stained with solution contained 2%
Alizarin Red (Sigma-Aldrich). ALP and Alizarin Red staining were measured by
using the Image-Pro Plus 6.0 program (Media Cybernetics, Rockville, MD,
USA).
Lon-BMSCs and Al-BMSCs sheets were incubated in the osteogenic medium
(Invitrogen). The medium was changed every 2 days. On day 5 after induction,
cells or cell sheets were fixed with 4% paraformaldehyde for alkaline
phosphatase (ALP) staining following the manufacturer’s protocol
(Sigma-Aldrich). On day 14 after induced for osteogenic induction, cells or cell
sheets were fixed with 70% ethanol, and stained with solution contained 2%
Alizarin Red (Sigma-Aldrich). ALP and Alizarin Red staining were measured by
using the Image-Pro Plus 6.0 program (Media Cybernetics, Rockville, MD,
USA).
3
Alizarin Red Staining of Induced MMSCs
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MMSCs were induced with MgCl2 as aforementioned. After 21 days of induction, cells were fixed with 4% paraformaldehyde for 10 min at room temperature, and washed once with distilled water. Subsequently, cells were stained with Alizarin Red (Beijing Solarbio Science & Technology Co., Ltd.) for 30 min at room temperature, washed with distilled water and observed under an IX71-F22FL/DIC optical microscope (Olympus Corporation; magnification, ×10). Alizarin Red staining analysis was conducted using Image-Pro Plus software (version 6.0; Media Cybernetics, Inc).
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