Methocult gf m3234

Manufactured by STEMCELL

MethoCult GF M3234 is a methylcellulose-based medium for the in vitro colony-forming cell (CFC) assay of human hematopoietic progenitor cells. It contains recombinant human growth factors to support the growth of granulocyte, erythroid, macrophage, and megakaryocyte colonies.

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MethoCult (224 citations) MethoCult M3234 (32 citations) MethoCult GF (18 citations) M3234 (18 citations)

3 protocols using methocult gf m3234

Clonogenic Progenitor Assay in Methylcellulose

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Clonogenic progenitors were determined in methylcellulose medium (MethoCult GF M3234, StemCell Technologies) with cytokines (SCF, TPO, EPO, IL-3 and GM-CSF) using 2 × 10⁴ BMMCs per well (6-well plate). Colonies were scored after 7 days of the initial culture, and all cells were collected and washed twice in phosphate-buffered saline. Subsequently cells were cultured at 2 ×10⁴ per well in the same medium. Colony scoring and replating were repeated every 7 days for at least two times, or until no colonies were observed in the cultures.

Kobayashi M., Bai Y., Dong Y., Yu H., Chen S., Gao R., Zhang L., Yoder M.C., Kapur R., Zhang Z.Y, & Liu Y. (2014). PRL2/PTP4A2 phosphatase is important for hematopoietic stem cell self-renewal. Stem cells (Dayton, Ohio), 32(7), 1956-1967.

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Clonogenic Assay for Bone Marrow Progenitors

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Clonogenic progenitors were determined in methylcellulose medium (MethoCult GF M3234, StemCell Technologies) using 2 × 10⁴ BM cells per well (6-well plate). Colonies were scored after 7 days of the initial culture, and all cells were collected and washed twice in PBS. Subsequently cells were cultured in the same medium. Colony scoring and replating were repeated every 7 days for at least two times^{20 (link),21 (link)}.

Chen S., Wang Q., Yu H., Capitano M.L., Vemula S., Nabinger S.C., Gao R., Yao C., Kobayashi M., Geng Z., Fahey A., Henley D., Liu S.Z., Barajas S., Cai W., Wolf E.R., Ramdas B., Cai Z., Gao H., Luo N., Sun Y., Wong T.N., Link D.C., Liu Y., Boswell H.S., Mayo L.D., Huang G., Kapur R., Yoder M.C., Broxmeyer H.E., Gao Z, & Liu Y. (2019). Mutant p53 drives clonal hematopoiesis through modulating epigenetic pathway. Nature Communications, 10, 5649.

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Clonogenic Assay for BMMCs

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Clonogenic progenitors were determined in methylcellulose medium (MethoCult GF M3234, StemCell Technologies) with cytokines (SCF, TPO, EPO, IL-3, and GM-CSF) using 2 x 10⁴ BMMCs per well (6-well plate). Colonies were scored after 7 days of the culture.

Kobayashi M., Nabinger S., Bai Y., Yoshimoto M., Gao R., Chen S., Yao C., Dong Y., Zhang L., Rodriguez S., Yashiro-Ohtani Y., Pear W.S., Carlesso N., Yoder M.C., Kapur R., Kaplan M.H., Lacorazza H.D., Zhang Z.Y, & Liu Y. (2017). Protein tyrosine phosphatase PRL2 mediates Notch and Kit signals in early T cell progenitors. Stem cells (Dayton, Ohio), 35(4), 1053-1064.

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