ELISA was used to determine anti-OVA or anti-SARS-CoV-2 spike IgG titers. Briefly, 96-well microtiter plates (Thermo Fisher) were coated with 2.0 µg/mL of OVA (Invivogen) or SARS-CoV-2 spike protein (RayBiotech) overnight at 4°C. Plates were washed with 0.05% Tween-20 in PBS (PBST) and blocked with 1% BSA/PBST. Mouse serum samples were two-fold serially diluted in PBST, added to the blocked plates, and incubated at 37 °C for 1 h. Following incubation, plates were washed with PBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 (Southern Biotech, cat#1070-05, 1:5000) or goat anti-mouse IgG2c (Southern Biotech, cat# PA1-29288, 1:4000) for 1 h. Plates were washed with PBST and TMB substrate (BD Bioscience) was added. Reactions were stopped with 50 µl 2 N H2SO4. Plates were read at OD 450 nm with a SpectraMax Plus plate reader (Molecular Devices). The antibody titer is defined as the dilution in which absorbance is more than 2.1 times of the blank wells.
Horseradish peroxidase hrp conjugated goat anti mouse igg1
Manufactured by Southern Biotech
Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG1 is a laboratory reagent used in immunoassays and other applications that require the detection of mouse IgG1 antibodies. It consists of goat-derived antibodies specific to the mouse IgG1 subclass, coupled to the enzyme horseradish peroxidase. This conjugate can be used to identify and quantify the presence of mouse IgG1 in samples through colorimetric or chemiluminescent detection methods.
Automatically generated - may contain errors
Lab products found in correlation
Sars cov 2 spike protein, by RayBiotech (1 mentions)
96 well microtiter plate, by Thermo Fisher Scientific (1 mentions)
Ovalbumin (ova), by InvivoGen (1 mentions)
Goat anti mouse igg2c, by Southern Biotech (1 mentions)
Tmb substrate, by BD (1 mentions)
Spectramax plus, by Molecular Devices (1 mentions)
Ultra tmb peroxidase substrate system, by Thermo Fisher Scientific (1 mentions)
Fluostar omega microplate reader, by BMG Labtech (1 mentions)
Lsrfortessa cell analyzer, by BD (1 mentions)
Hrp conjugated goat anti mouse iga, by Southern Biotech (1 mentions)
Np9 bsa, by Biosearch Technologies (1 mentions)
3 protocols using horseradish peroxidase hrp conjugated goat anti mouse igg1
1
Quantifying Anti-OVA and Anti-SARS-CoV-2 IgG Titers
2
Flow Cytometry and ELISA for Murine Immunoglobulins
3
ELISA-Based Anti-NP IgG Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-NP IgG antibody levels were quantified by ELISA using NP(9)-BSA (Biosearch
Technologies) as the capture antigen in the in vitro or in vivoantibody production assay, respectively. ELISA plates were prepared using the
Immuno-Tek ELISA construction system (Zepto Metrix) according to the
manufacturer’s protocol. Following the incubation with sample serum
or media, the plates were developed with horseradish peroxidase (HRP)-conjugated
goat anti-mouse IgG1, or HRP-conjugated goat anti-mouse IgA (SouthernBiotech),
and TMB substrate. Serially diluted pooled sera from NP(13)-OVA-immunized B6
mice were included as controls on each plate. The concentrations of the anti-NP
IgG1 antibody were estimated by comparisons with standard curves constructed
from pooled sera.
Technologies) as the capture antigen in the in vitro or in vivoantibody production assay, respectively. ELISA plates were prepared using the
Immuno-Tek ELISA construction system (Zepto Metrix) according to the
manufacturer’s protocol. Following the incubation with sample serum
or media, the plates were developed with horseradish peroxidase (HRP)-conjugated
goat anti-mouse IgG1, or HRP-conjugated goat anti-mouse IgA (SouthernBiotech),
and TMB substrate. Serially diluted pooled sera from NP(13)-OVA-immunized B6
mice were included as controls on each plate. The concentrations of the anti-NP
IgG1 antibody were estimated by comparisons with standard curves constructed
from pooled sera.
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