Cells (5 × 104 cells/well) were seeded in a 96-well plate and grown overnight. Next, cells were treated with vehicle, rotenone (1 μM), and 0–100 ng/ml recombinant human GDF-15 (57-GD-025, R&D Systems, United States). Cell viability was detected using a Cell Counting Kit (CCK) 8 assay (A311-02, Vazyme, Nanjing, China). Briefly, after 24 h, 10 μL of CCK8 solution was added to each well and the incubation continued for another 4 h at 37°C. Absorbance was measured using a multimode microplate reader (Thermo Fisher Scientific Inc., MA, United States) at 450 nm. Cell viability was expressed as a percentage relative to the absorbance of control cells.
Multimode microplate reader
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
The Multimode Microplate Reader is a versatile laboratory instrument designed for a wide range of absorbance, fluorescence, and luminescence assays. It provides reliable and accurate measurements of samples in microplates, allowing researchers to analyze a large number of samples simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
Cck 8 solution, by Beyotime (2 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions)
Cell counting kit 8 cck 8 assay, by Dojindo Laboratories (2 mentions)
Lipid peroxidation mda assay kit, by Beyotime (2 mentions)
Multiskan fc, by Thermo Fisher Scientific (2 mentions)
Cell counting kit 8 assay, by Vazyme (1 mentions)
E1016, by Applygen (1 mentions)
E1015, by Applygen (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Transwell plate, by Corning (1 mentions)
Fluorescence microscope, by Leica (1 mentions)
Bca kit, by Solarbio (1 mentions)
Dcfh da probe, by Meilun (1 mentions)
S1 protein, by Sino Biological (1 mentions)
Recombinant sars cov 2 spike protein, by Sino Biological (1 mentions)
Cck 8 solution, by Dojindo Laboratories (1 mentions)
Total superoxide dismutase assay kit with nbt, by Beyotime (1 mentions)
Evans blue dye, by Merck Group (1 mentions)
D7000, by Nikon (1 mentions)
43 protocols using multimode microplate reader
1
GDF-15 Modulates Rotenone-Induced Cytotoxicity
2
Evaluating Endothelial Permeability via FITC-Dextran
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Endothelial permeability assays were performed using FITC-dextran (70 kDa) extravasation for quantification across HBMECs seeded in transwell chambers, as previously described [16 (link)]. Briefly, HBMECs were seeded at 1×105 cells/well in 200 μL of complete ECM in 24-well polycarbonate transwell chambers with 0.4 μm pores. After 48 h, the cells were cultured until they reached confluence and were treated with or without OGD/R, bFGF, PD173074, and PD98059. The medium was replaced with medium containing 1% FITC-dextran (1 mg/mL), and the cells were incubated for 4 h. Finally, the relative fluorescence was measured that crossed the lower chamber using a multimode microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) at wavelengths of 485 nm for excitation and 520 nm for emission.
3
Cell Viability Assessed by CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCK8 assay was applied to measure the viability of CHON-001 cells. Cell were seeded into 96-well plates and then incubated with CCK-8 solution (Beyotime, Shanghai, China) at 37 °C for 2 h. MultiMode Microplate Reader (Thermo Fisher Scientific) was applied to collect and analyze the signal about the absorbance at 450 nm.
4
Cell Viability Assay of PMNs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Cell-Counting Kit 8 (CCK8; Beyotime Biotechnology, China) was used to test relative cell viability according to the manufacturer’s instructions. Briefly, PMNs were seeded in 96-well plates (2 × 103 cells/well), which were divided into ATA-treated groups (containing PMNs with ATA concentrations of 5, 10, 15, 20, 25, and 30 μM), control group, and blank group with five holes in each group. The control group contained PMNs and medium, and the blank group only contained medium. After 3 h incubation, 10 μl CCK-8 solution was added and incubated in the incubator for 1 h. The absorbance at 450 nm was determined in a multimode microplate reader (Thermo Fisher Scientific, USA).
5
UV Absorbance Spectra of Cu-Glu Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ultraviolet (UV) absorbance spectra of Cu Glu in deionized water, free PSD in DMSO, Cu2+–PSD complex in DMSO and PSD LPs in deionized water were obtained using a multimode microplate reader (Thermo Scientific, USA) within the wavelength range of 400–650 nm.
6
Enzymatic Cholesterol Quantification in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An enzymatic assay was used for determining the content of total cholesterol and free cholesterol levels of cells according to the manufacturer’s instructions (Applygen Technologies, E1015 and E1016, Beijing). Briefly, cells were washed with PBS to remove the remaining serum from the culture medium and then added with lysis buffer in the kit. The working solution and cholesterol standard substance were diluted. After incubation at 37 °C for 20 min, OD values were measured at 550 nm by multimode microplate reader (Thermo Fisher, USA) to calculate cholesterol content.
7
Evaluating Anti-Parkinsonism Agents using 6-OHDA-Induced PC-12 Cell Toxicity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PC-12 cells were obtained from Hunan Fenghui Biotechnology Co., Ltd. (Changsha, China). The 6-hydroxydopamine (6-OHDA) was used because it is a toxic oxidative metabolite of dopamine and has been used effectively to evaluate potential anti-Parkinsonism agents [53 (link)]. The cells were cultured in DMEM supplemented with 5% FBS and 1% penicillin-streptomycin, and maintained at 37 °C with 5% CO2. The cells were trypsinized when they achieved over 90% growth on the bottom and seeded in 96-well microplates (1 × 104 cells/well) to incubate in the same medium and environment as above. After being cultured for 24 h, PC-12 cells were treated with 100 μL of each test compound at various concentrations (25 μM, 50 μM and 100 μM) for 2 h, and then the cells were incubated with 6-OHDA for another 24 h. At the same time, rasagiline was used as the positive control in this experiment because safinamide failed to exhibit significant protective effects on PC-12 cells as rasagiline. The optical densities were measured at 450 nm using a multimode microplate reader (Thermo Fisher, Waltham, MA, USA).
8
Assessing Cell Viability with Crystal Violet
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HP14-19-CD cells were infected with adenovirus Ad-Cre (12 (link)) to remove the HSV-tk gene, then cultured in complete Dulbecco's modified Eagle's medium with 10% FBS containing various concentrations (0, 0.1, 0.2, 0.5, 1, 2, 5 and 10 µg/ml) of GCV (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). HP14-19 and HP14-19-CD cells were treated with various concentrations (0, 10, 20, 40 and 80 µg/ml) of 5-FC (Sigma-Aldrich; Merck KGaA). HP14-19-CD cells which were infected with Ad-green fluorescent protein served as control group. Crystal violet staining was performed in order to assess cell viability. Cells were incubated in 24-well plates at 2.0×104 cells/well and analyzed after 1 week. Briefly, the culture medium was removed and the cells were fixed with 4% paraformaldehyde at room temperature for 10 min, then stained with 0.05% crystal violet for 30 min. Following washing with tap water, cells were dried on filter paper. The blue dye was dissolved in 500 µl methanol and determined at an excitation wavelength of 540 nm using a Multimode Microplate Reader (Thermo Fisher Scientific, Inc.). Three independent experiments were performed in triplicate. The mean and standard deviation were calculated.
9
Thyroid Cancer Cell Line Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The thyroid cancer cell lines BCPAP and KHM-5M were purchased from the Cell Culture Collection of the Chinese Academy of Sciences (Shanghai, China), and both were cultured in RPMI-1640 (Gibco) with 10% fetal bovine serum (Gibco). Cells were cultivated at 37 °C with 5% CO2. For colony formation assay, cells were seeded in 6-wells plate for 2 weeks. Colonies were then fixed with 4% paraformaldehyde for 15 min and stained with crystal violet for 10 min. For cell proliferation assay, cells were plated in 96-well plates, and incubated with Cell Counting kit-8 assay (CCK8, Dojindo Laboratories) for 2 h. We recorded the absorbance of each well with a Multimode Microplate Reader (Thermo).
10
ABTS Radical Scavenging Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The radical scavenging activities of crude PCPP and PCPP-1a against ABTS+ were measured by an ABTS assay kit using the method in line with the manufacturer's instructions. Absorbance was measured at 414 nm using a microplate reader in a Multimode Microplate Reader (Thermo Scientific, USA), with Trolox as an antioxidant standard. The antioxidant activity of the sample was calculated using where AB and AA represent the absorbance values of the blank and of the tested samples, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!