Pd2(dba)3 and P(tolyl)3 were purchased from Sigma-Aldrich (USA). TTBDT (4,8-Bis(5-(2-ethylhexy)thieno[3,2-b]thiophen-2-yl)benzo[1,2-b:4,5-b]dithiophene-2.6-diyl)bis(trimethylstannane)) was purchased from Suna Tech Inc. (Suzhou, China). Tetrahydrofuran, methanol, dichloromethane and toluene were purchased from Sinopharm Chemical Reagent Co. Ltd. (China). 1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy[poly(ethylene glycol)]-2000] (DSPE-mPEG2000) was purchased from A.V.T Pharmaceutical (Shanghai, China). DiO and DiR were purchased from Us Everbright Inc. (Suzhou, China). APC-affinity anti-CD11b-antibody was purchased from Thermo Fisher Scientific (USA). Hoechst 33342 was purchased from Sigma-Aldrich (USA). Cell Counting Kit-8 (CCK-8) and the Annexin V-FITC/PI apoptosis detection kit were purchased from Dojindo Laboratories (Kumamoto, Japan). Calcein AM and PI were purchased from J&K Scientific Ltd (Beijing, China). Ultrapure water was prepared by a Milli-Q Gradient System (18.2 M Ω resistivity, Millipore Corporation, Bedford, MA, USA).
Dichloromethane
Manufactured by Sinopharm
Sourced in China, United States, Argentina
Dichloromethane is a colorless, volatile, and versatile organic solvent. It has a wide range of applications in various industries, including chemical synthesis, pharmaceutical production, and laboratory analysis. As a solvent, dichloromethane is characterized by its high solvency, low boiling point, and low surface tension, making it suitable for a variety of extraction, purification, and cleaning processes.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Sinopharm (34 mentions)
Ethanol, by Sinopharm (25 mentions)
Toluene, by Sinopharm (20 mentions)
Acetone, by Sinopharm (16 mentions)
Ethyl acetate, by Sinopharm (15 mentions)
Sodium hydroxide (naoh), by Sinopharm (15 mentions)
Hydrochloric acid (hcl), by Sinopharm (14 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (14 mentions)
N n dimethylformamide (dmf), by Sinopharm (12 mentions)
Tetrahydrofuran, by Sinopharm (11 mentions)
Sodium chloride (nacl), by Sinopharm (11 mentions)
Hexane, by Sinopharm (9 mentions)
Petroleum ether, by Sinopharm (9 mentions)
Anhydrous sodium sulfate, by Sinopharm (9 mentions)
Triethylamine, by Sinopharm (9 mentions)
N hexane, by Sinopharm (9 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (9 mentions)
Cyclohexane, by Sinopharm (8 mentions)
Acetonitrile, by Sinopharm (8 mentions)
Chloroform, by Sinopharm (7 mentions)
134 protocols using dichloromethane
1
Synthesis and Characterization of TTBDT-based Nanoparticles
2
Functionalized Mesoporous Silica Nanoparticles for Drug Delivery
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To minimize the batch difference of produced composites, mesoporous silica nanoparticle (MSNs) and amino-functionalized MSNs (MSNs-NH2) were obtained from XFNANO industry. Bovine α-lactalbumin (calcium depleted, ≥85% purity) was purchased from Sigma-Aldrich. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDC), oleic acid, docetaxel, N-Hydroxysuccinimide (NHS) and 8-anilinonaphthalene-1-sulfonic acid (ANS) were from Shanghai Aladdin biochemical technology. Acetonitrile of HPLC-grade, anhydrous ethanol and dichloromethane were from Sinopharm Chemical Reagent. Dialysis membrane (molecular mass cutoff of 8–14 kDa) was obtained from Henghuibio technology development co., LTD. Fetal bovine serum (FBS), Dulbecco's modified Eagle's medium (DMEM), penicillin-streptomycin (10000 U/ml) were purchased from Gibco (Thermo Fisher Scientific). Cell counting kit-8 (CCK-8), JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazoly l-carbocyanineiodide) and Mitochondrial membrane potential assay kit were purchased from MedChemExpress. Dithiothreitol (DTT), 4′, 6-diamidino-2-phenylindole (DAPI) and 0.25% trypsin-ethylenediaminetetraacetic acid (EDTA) were purchased from Beyotime Institute of Biotechnology. The assay solutions were prepared using deionized water. All other chemicals and reagents were of analytical grade unless otherwise specified.
3
Formulation and Characterization of DTX-PLGA Nanoparticles
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DTX was purchased from Biocompounds Pharmaceutical Inc. (Shanghai, China). PLGA (Mw = 15 kDa) was purchased from Jinan Daigang Biological Tech Co., Ltd. Borneol was purchased from Lei Yunshang Drug Store. PEI (Mw = 1.8kDa), Folic acid (USP grade), 1-ethyl-3-(3-dimethylamino propyl) carbodiimide hydrochloride (EDC.HCl, purity > 98%), N-hydroxysuccinimide (NHS, purity > 98%), succinic anhydride (SA, purity > 98%), 4-(dimethylamino) pyridine (DMAP) were provided by Aladdin Chemistry (Shanghai, China). Coumarin-6 was obtained from Sigma-Aldrich (St. Louis, MO, USA). Rhodamine123 (R123) (purity ≥ 95%) was obtained from Wako Pure Chemical Industries, Ltd. Dichloromethane was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), triethylamine (TEA), N,N-Dimethylformamide (DMF) and other solvents were analytical grade reagents and obtained from Shanghai Lingfeng Chemical Reagent Co., Ltd. Fetal bovine serum (FBS), Dulbecco’s phosphate buffer saline (PBS), Trypsin 0.25%-EDTA and culture medium DMEM were purchased from Biosera (Biosun Science & Technology Co., Ltd., Shanghai, China). Caco-2 cell lines (Caco-2) were provided by Cell Bank of Chinese Academy of Sciences (Shanghai, China).
4
Synthesis and Characterization of Glucose-Functionalized Polymers
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Monohydroxy-capped poly(ethylene glycol) (PEG113-OH) with a mean degree of polymerization, DP, of 113 (Mn = 5000 g/mol, Mw/Mn = 1.02) was purchased from Aldrich and dried at 30 °C under vacuum overnight prior to use. 2-Diethylaminoethyl methacrylate (DEA, Aldrich, 99%) and styrene (St, Aldrich, 99%) were passed through basic alumina column to remove inhibitor and then vacuum distilled from CaH2 and stored at − 20 °C prior to use. 2, 2-Bipyridine (bpy), copper bromide (CuBr), 2-bromoisobutyryl bromide, 2-aminoethyl methacrylate hydrochloride (AMA.HCl), d -glucono-δ-lactone, bortezomib (BTZ), and Alizarin Red S (ARS) were all purchased from J&K and directly used. Methanol, toluene, triethylamine (TEA), 1-methyl-2-pyrrolidinone (NMP), and isopropyl alcohol (IPA) were purchased from Sinopharm Group Chemical Reagent Co., Ltd., and then distilled from CaH2 under reduced pressure. Dichloromethane, chloroform, dimethyl sulfoxide (DMSO), and other organic solvents were purchased from Sinopharm Group Chemical Reagent Co., Ltd., and directly used without further purification. Bromide terminated poly(ethylene glycol) (PEG113-Br) macroinitiator and 2-gluconamidoethyl methacrylate (GAMA) monomer were prepared from PEG113-OH and 2-bromoisobutyryl bromide, and AMA.HCl and d -glucono-δ-lactone, respectively, according to literature procedures [35 (link), 36 (link)].
5
Multiplex Analysis of Veterinary Drugs
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Standard chemicals, namely, danofloxacin, erythromycin, sulfadimidine, benzylpenicillin potassium, chloramphenicol, lincomycin, tetracycline, spectinomycin, thiamphenicol, dexamethasone, kanamycin, melamine, trimethoprim, zearanol, aflatoxin M1, flunixin meglumine, and florfenicol, were purchased from Sigma (St. Louis, MO, USA). All the chemicals used were of analytical grade. Lead acetate, potassium oxalate, disodium hydrogen phosphate, trichloroacetic acid, sodium hydroxide, and dichloromethane were obtained from Sinopharm Chemical Reagent Co., Ltd. (China). High-purity water was obtained from a Milli-Q water system (Millipore, Billerica, MA, USA). Pure milk was purchased from a local supermarket. Commercialized semiquantitative ICA strip and an ICA strip reader were supplied by Beijing Meizheng Bio-Tech Co., Ltd. (Beijing, China)
6
Analytical Reagents for Furfural Conversion
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Analytical reagents, including furfural, dichloromethane, tetrahydrofuran, methyl alcohol, ethyl alcohol, sodium hydroxide, sodium carbonate, aluminium nitrate nonahydrate, magnesium nitrate hexahydrate, cobaltous nitrate hexahydrate, ziconyl nitrate, calcium oxide, magnesium oxide, gamma aluminum oxide and potassium fluoride dihydrate were purchased from Sinopharm Chemical Reagent Co., Ltd. Cyclopentanone (99%) was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. F2C standard was prepared according to the report,30 (link) and verified by nuclear magnetic resonance (NMR). furfural was directly used without any preliminary treatment considering the same experimental result.25 (link)
7
Grass Carp Processing and Analysis
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Live grass carp (2.5–3.0 kg) was purchased from the Huazhong Agricultural University market (Wuhan, China). Arowana multipurpose wheat core wheat flour (Yihai Kerry Arowana Cereals and Oils Food Co., Ltd., Shanghai, China), MT200 modified starch (Tianjin Dingfeng Starch Development Co., Ltd., Tianjin, China), konjac glucomannan (food grade) (Hefei Shengrun Biological Products Co., Ltd., Hefei, China) and rapeseed oil (Shandong Luhua Group Co., Ltd., Yantai, China) were used. Salt and baking powder were purchased from Zhongbai Supermarket of Huazhong Agricultural University (Wuhan, China).
Petroleum ether (30–60 °C), HCl, chloroform, Sudan Red B, acetone, NaBH4, methanol, thiobarbituric acid, disodium EDTA, 1,1,3,3-tetraethoxypropane, formic acid, dichloromethane and hexane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Carboxymethyl lysine (CML), carboxyethyl lysine (CEL) and methylglyoxalimidazolone (MG-H1) standards were purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Acrylamide standard was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
Petroleum ether (30–60 °C), HCl, chloroform, Sudan Red B, acetone, NaBH4, methanol, thiobarbituric acid, disodium EDTA, 1,1,3,3-tetraethoxypropane, formic acid, dichloromethane and hexane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Carboxymethyl lysine (CML), carboxyethyl lysine (CEL) and methylglyoxalimidazolone (MG-H1) standards were purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Acrylamide standard was obtained from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA).
8
PLLA Synthesis and Lung Cancer Evaluation
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PLLA (Mw 50,000) was purchased from Jinan Daigang Biomaterial Co., Ltd. (Jinan, China). Menthol was purchased from Aladdin (Los Angeles, CA, USA). Dichloromethane (DCM, 99.8% purity) and ethanol (99.8% purity) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Alamar blue was purchased from Invitrogen (Carlsbad, CA, USA). Human lung adenocarcinoma cell line A549 cells were obtained from Nanjing KeyGEN Biotech Co., Ltd. (Nanjing, China). SD rats were obtained from the Slac Laboratory Animal Co., Ltd. (Shanghai, China). A lactate dehydrogenase (LDH) kit was purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other compounds were of analytical purity.
9
Lung Cancer Cell Line Characterization
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Human lung cancer cell lines (A549, H1975, H460, H446) and human umbilical vein endothelial cell line (HUVEC) were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. Fe3O4 solution, carboxymethyl chitosan hexadecyl quaternary ammonium salt (HQCMC), 1,2-dioleoylphosphatidylcholine (DOPC), and dimethyloctadecylglycidyl ammonium chloride (GHDC) were purchased from Huzhou Lieyuan Medical Laboratory Co, Ltd dulbecco’s modification of eagle’s medium dulbecco (DMEM)/1640 medium, fetal bovine serum, and trypsin were purchased from Gibco. Epithelial cell adhesion molecule, vimentin, and EGFR antibodies were purchased from eBioscience. Cholesterol, dichloromethane, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylammoniumpropyl) ammonium carbonate (EDC), and other common reagents were purchased from Sinopharm Group. Prussian blue staining kit was purchased from Solarbio.
10
Thiols Characterization and Quantification
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The thiols investigated were methanethiol, ethanethiol, 2-mercapto-1-ethanol, 2-furfurylthiol, 2-methyl-3-furanethiol, and 2-phenylethanethiol. Commercially available high-purity analytes (96%) were obtained from Sigma-Aldrich (Shanghai, China). DTDP (97%) was purchased from Sigma-Aldrich (Shanghai, China). Ethylenediaminetetraacetic acid disodium salt (EDTA-Na2), acetaldehyde (99%), and formic acid (99%) were purchased from J&K Chemical Corp., (Beijing, China). Acetonitrile (ACN, LC-MS, HPLC grade), methanol (MeOH, HPLC grade), ethanol (EtOH > 99.8%), ethyl acetate (EAC, HPLC grade), dichloromethane (DCM, HPLC grade), Anhydrous magnesium sulfate (MgSO4), sodium chloride (NaCl), sodium citrate tribasic dihydrate (C6H5Na3O7·2 H2O, 99.0%), and sodium citrate dibasic sesquihydrate (C6H6Na2O7·1.5 H2O, ≥ 99.0%) were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Aminopropyl-modified silica (NH2), graphitized carbon black (GCB), and octadecyl-modified silica (C18) were purchased from the ANPEL Laboratory Technologies (Shanghai, China). Ultra-pure water (18 MΩ cm) was obtained using a Milli-Q water purification system (Millipore, Billerica, MA, USA).
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