CSF was obtained by lumbar puncture between the L3/L4 and L4/L5 intervertebral space and collected in polypropylene tubes (Sarstedt, Nümbrecht, Germany). CSF samples were centrifuged within 2 hours at 1800g at room temperature (RT) for 10 minutes and stored at −80°C in aliquots of 0.5 or 1 mL in Sarstedt polypropylene tubes according to BioMS-eu guidelines.21 (link)
Polypropylene tube
Manufactured by Sarstedt
Sourced in Germany, United States, United Kingdom
Polypropylene tubes are a type of laboratory equipment commonly used for storing, transporting, and processing various samples and solutions. These tubes are made of polypropylene, a durable and chemically resistant plastic material. Polypropylene tubes are designed to provide a secure and leak-proof containment for a wide range of applications in scientific and medical research, as well as clinical diagnostics.
Automatically generated - may contain errors
Lab products found in correlation
Innotest, by Fujirebio (8 mentions)
Innotest β amyloid1 42, by Fujirebio (3 mentions)
Innotest elisa, by Fujirebio (3 mentions)
Innotest phospho tau 181p, by Fujirebio (2 mentions)
Innotest sandwich elisa, by Fujirebio (2 mentions)
Serum gel with clotting activator tube, by Sarstedt (2 mentions)
Dimethyl malonate, by Thermo Fisher Scientific (2 mentions)
Mini beadbeater, by Biospec (1 mentions)
Glass beads, by Carl Roth (1 mentions)
Sandwich elisas, by Fujirebio (1 mentions)
K2 edta tube, by BD (1 mentions)
Inno bia alzbio3 kit, by Fujirebio (1 mentions)
Innotest htau ag, by Fujirebio (1 mentions)
Aim 5 medium, by Thermo Fisher Scientific (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Dynabeads human t activator cd3 cd28, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by BD (1 mentions)
Polypropylene tube, by BD (1 mentions)
Alzbio3 kit, by Fujirebio (1 mentions)
Biophotometer 6131, by Eppendorf (1 mentions)
91 protocols using polypropylene tube
1
CSF Collection and Processing Protocol
2
Biobanking of CSF and Plasma Samples
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CSF and plasma samples were collected between 8 a.m. and 10 a.m. after an overnight fast. CSF was collected in 10 mL polypropylene tubes (Sarstedt, Newton, NC, USA, 62.610.201). The tubes were inverted several times and centrifuged at 2000× g for 10 min at room temperature. The samples were aliquoted into two 2 mL polypropylene tubes (Sarstedt, 72.694.007), with each tube containing 1 mL of CSF. Blood samples were collected in EDTA-containing vacutainer tubes and centrifuged at 2000× g for 10 min at 4 °C to separate the plasma and buffy coat. All the samples were aliquoted and immediately stored at −80 °C until use. Samples were obtained with support from the IRBLleida Biobank, Lleida, Spain (B.0000682) and PLATAFORMA BIOBANCOS, Barcelona, Spain PT17/0015/0027 following the guidelines of Spanish legislation on this matter (Real Decreto 1716/2011).
3
Lumbar Puncture for Cerebrospinal Fluid Collection
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Lumbar puncture was performed before treatment switch and at months 12 and 24. Cerebrospinal fluid was collected in 10 ml polypropylene tubes (Sarstedt) and centrifuged at 400g for 10 minutes. The supernatant was pipetted off and dispensed in 9 fractions of 1 ml in 1.5 ml polypropylene tubes (Sarstedt) and stored at -80°C.
4
CSF Surplus Sample Processing Protocol
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CSF surplus samples (e.g., samples having inadequate clinical information for use in clinical validation) from the Amsterdam Dementia Cohort (ADC) were used for this study [24 (link)]. The protocol was approved by the institutional review board (reference number: 2017.315) and subjects gave written consent. ADC CSF was collected by lumbar puncture in 10 mL polypropylene tubes (Sarstedt, EU # 62.610.018). CSF samples were centrifuged at 1800 to 2100 g for 10 min at 4°C within 2 h after collection, aliquoted into polypropylene tubes (1.5 or 2.0 mL; Sarstedt, EU 72.703 or 72.694.007) in 500μL volumes [25 (link)]. Subsequently, either freshly collected or CSF surplus samples stored at –80°C, were used to perform the specific experimental sample treatments as described below.
5
Plasma Isolation from Venous Blood
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Samples were collected following international guidelines for biobanking (Teunissen et al., 2009 (link)). Venous blood was drawn from a cubital vein into evacuated K2-EDTA or heparinzed tubes. Hereafter, blood was centrifuged within 30–60 min after collection at 2000 G for 10 min at 20 °C. Plasma was aliquoted in 500 μL Sarstedt polypropylene tubes and stored at −80 °C until batch analysis (Teunissen et al., 2009 (link)).
6
Intracellular Vitamin B Analysis in Bacteria
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Sample preparation for analysis of intracellular vitamin B content in bacteria was performed using a slightly modified version of the method described for the analysis of bacterial coenzyme a thioesters [21 (link)]. Frozen cell pellets were resuspended in 1 mL of methanol, transferred into 2 mL polypropylene tubes (Sarstedt) containing 0.5 mL glass beads (0.7 and 0.1 mm, Carl Roth), and broken up by homogenization in a mini-bead beater (biospec products). In addition, the extraction was repeated twice with 0.5 mL of methanol, and the solvent was evaporated under a gentle flow of nitrogen gas. All other steps were performed according to Cakić et al. [21 (link)]. The filtered solution was analyzed by liquid chromatography coupled with mass spectrometry. Bacterial vitamin concentrations were calculated by external quantification, as previous tests for recovery and matrix effect have shown that the analytes were all recovered at 98–100% with no ion suppression.
7
CSF Biomarkers in Dementia with Lewy Bodies
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Concomitant AD pathology in DLB patients, hereinafter referred to as DLB-AD, was defined as a ratio of CSF phosphorylated tau (Ptau)/Aβ1–42≥0.054 (Willemse EAJ, in preparation). CSF was obtained by lumbar puncture between the L3/L4, L4/L5, or L5/S1 intervertebral space using a 25-gauge needle and a syringe and collected into 10 mL polypropylene tubes (Sarstedt, Nümbrecht, Germany), following the international biobanking consensus guidelines for CSF [34 (link)]. CSF was routinely analyzed for levels of Aβ1–42, total tau, and p-tau with commercial Enzyme-linked immunosorbent assays (ELISA) (Innotest®, Fujirebio, Gent, Belgium). Aβ1–42 measures were adjusted for an upward drift over time as previously described [35 (link)]. Of the 190 patients with DLB, 154 (81%) patients had CSF measures of Aβ1–42 and total tau.
8
CSF Biomarker Measurement Protocol
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CSF was obtained by lumbar puncture, collected in polypropylene tubes (Sarstedt, Nurmberg, Germany), and processed according to international guidelines [25 (link)–27 (link)]. Abeta (1-42) and phosphorylated tau (p-tau) concentrations were measured using sandwich ELISAs (Innotest, Fujirebio, Gent, Belgium [28 (link)]. We adjusted Abeta concentrations for upward drift [29 (link)].
9
Photodynamic Therapy Assay Protocol
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(8 x 10 5 ) in serum free medium were added to 5 mL polypropylene tubes (Sarstedt, UK) containing duramycin-porphyrin conjugate at required concentration (0-10 µM) and incubated in the dark at 37 °C in a 5% CO2 atmosphere for 5 minutes. Cells were then washed with 3 mL medium so as to remove any unbound conjugate and centrifuged at 300g for 10 minutes and re-suspended in 1 mL medium. Cells (8 x 10 4 ) were then plated into 96 well plates and incubated in the dark at 37 °C in a 5% CO2 atmosphere for 5 minutes. The PDT light supply (9x14 LED Array, wavelength 620-660 nm) was allowed to stabilise and irradiance reading taken using a R203 Macam radiometer (Irradian Ltd. UK). After incubation one plate was irradiated with a light fluence of 7.5 J/cm 2 red light while the other served as the dark control (i.e. not irradiated). After light treatment 5 µl FBS was added to each well and the plates incubated at 37 °C in a 5% CO2 atmosphere for 24 hours.
10
Measuring Cognitive Function and AD Biomarkers
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Global cognition was measured with the MMSE, with a maximum score of 30 and a higher score indicating better global cognition [16 (link)]. AD biomarkers β-amyloid 42 (Aβ42), total tau (tau), and phosphorylated tau (p-tau) were measured in cerebrospinal fluid (CSF). CSF was obtained by lumbar puncture using a 25-gauge needle and collected in 10 ml polypropylene tubes (Sarstedt) following standardized protocols [26 (link)]. Aβ42, tau, and p-tau concentrations were determined with sandwich ELISAs (Fujirebio, Ghent, Belgium) [27 (link)]. Aβ42 concentrations were adjusted for the drift that occurred over the years [28 (link)], and data were available of 148 participants.
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