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Sz16 stereomicroscope

Manufactured by Olympus

The SZ16 stereomicroscope is a versatile and high-quality optical instrument designed for a variety of applications. It features a binocular observation tube and a magnification range of 6.7x to 90x, making it suitable for detailed inspection and analysis tasks. The SZ16 utilizes a system of lenses and prisms to provide a three-dimensional, upright image, allowing for natural and comfortable observation.

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4 protocols using sz16 stereomicroscope

1

New Millipede Species Discovery and Characterization

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Material was collected from different provinces in China (Table 1, Fig. 2). These specimens were preserved in 75% ethanol, and genomic DNA was extracted from the leg tissue. An Olympus E–M10 II camera was used to capture live colour patterns. Holotypes and paratypes of the new species were maintained deposited at the Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, China (CMMI).
The morphological terminology follows Bonato et al. (2010) (link). The taxonomic characteristics were observed using an Olympus SZ16 stereomicroscope. Helicon Focus 6.7.1 was used to create a multi-focused montage of pictures. The ArcMap 10.7.1 software tool was used to produce the maps.
Abbreviations: VL = ventrolateral, VM = ventromedial, M = medial, DM = dorsomedial, CS = corner spine, T = tergite, TT = tergites, SS = sternites, spm = specimen, coll. = collector, LBS = leg bearing segment.
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2

Fluorescence Microscopy of Cell and Tissue Samples

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For fluorescence microscopy of cell cultures, a Zeiss Axiovert 35M microscope equipped with fluorescence optics was used. For specific excitation of Venus a filter block with excitation of 450–490 nm and emission of 520–550 nm, for specific excitation of tdTomato a filter block with excitation of 530–570 nm and emission of 590–610 nm were used.
For imaging of tissue biopsies an Olympus SZ16 stereomicroscope with epifluorescence optics was used. For macroscopic imaging of the calves, either blue or green flood lights of light emitting diodes (LED; Eurolite) were used for excitation, and images were recorded with a digital camera and appropriate emission filters (Lee Filter)14 (link).
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3

Taxonomy and Morphology of Oriental Centipedes

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The studied individuals of Mimopsorientalis were collected in Taiping National Forest Park, Shaanxi, China, and Funiushan National Nature Reserve, Henan, China. Other representative samples of Cryptopidae, Scolopendridae, Scolopocryptopidae, and Plutoniumidae were collected more generally in China. During the field study, some individuals were photographed with an Olympus E-M10 II camera to record their living colour pattern. The colour description is based on that of living centipedes. Specimens are deposited in the Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, China (CMMI).
The taxonomic characteristics of the specimens were observed under an Olympus SZ16 stereomicroscope. Multifocus montage images were produced using Helicon Focus 6.7.1 software from a series of source images taken by a Canon 50D digital camera attached to the stereomicroscope. Terminology applied to the external anatomy follows Bonato et al. (2010) (link).
All newly collected centipede specimens were stored in 75% ethanol. Legs 15 and 17 of the newly collected specimens were removed and stored in 100% ethanol for DNA extraction.
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4

Multimodal Imaging of Device Structures

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Optical images of the various devices were taken using a SZ16 Stereomicroscope (Olympus) equipped with an Infinity 2 CCD camera (Lumenera). Computer tomography (CT) scans were obtained on a MicroCT 35, ScanCo Medical, Brüttisellen, Switzerland. The following conditions were used: X-ray tube potential 70 kVp, integration time 300 ms, X-ray intensity 145 μA, isotropic voxel size 12 um, frame averaging 1, projections 500, medium resolution scan. CT scans were converted to DIACOM format and segmented in Mimics Research V21.0 (Materialise). Scanning electron micrographs were acquired on a FEI Inspect F using a10 kV applied voltage, spot size of 1.0, working distance of 10.5 mm, and a dwell time of 3 μs.
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