The antibodies used in this work include the following commercial antibodies: anti-phospho-histone H2AX (Ser139) (#80312-mouse), anti-phospho-53BP1 (Ser1778) (#2675-rabbit), anti-phospho-ATR (Thr1989) (#30632-rabbit), anti-phospho-Rad50 (Ser635) (#14223-rabbit) and anti-GAPDH (#3683-rabbit-HRP conjugated) from Cell Signaling Technology, USA. Other commercial antibodies include anti-phospho-(Ser/Thr) ATM/ATR Substrate (#AP0933-rabbit), anti-phospho-PRKDC (Ser2056) (#AP0621-rabbit) and anti-phospho-ATM (Ser1981) (#AP0008 -rabbit) from ABclonal, USA. Methyl methanesulfonate (MMS, Sigma, USA), DNA-PK inhibitor (AZD7648, AstraZeneca, UK), generously provided by Dr. Jennifer A. Black (Dept of Cellular and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, USP).
Anti phospho rad50 ser635
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho-Rad50 (Ser635) is a laboratory reagent used to detect the phosphorylation of the Rad50 protein at serine 635. This antibody can be used in various techniques, such as Western blotting, to analyze the phosphorylation status of Rad50 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti phospho histone h2ax ser139, by Cell Signaling Technology (1 mentions)
Anti phospho atr thr1989, by Cell Signaling Technology (1 mentions)
Methyl methanesulfonate mms, by Merck Group (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
Anti sp1, by Merck Group (1 mentions)
Amersham hyperfilm ecl, by GE Healthcare (1 mentions)
Anti sod2, by GeneTex (1 mentions)
Anti tubulin, by Proteintech (1 mentions)
Anti mgmt, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
2 protocols using anti phospho rad50 ser635
1
DNA Damage Response Protein Phosphorylation
2
Western Blot Analysis of Protein Targets
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Protein samples were separated by SDS-PAGE, and transferred onto a PVDF membrane (Bio-Rad Laboratories). Subsequently, the PVDF membrane was blocked for 1 h by using 5% nonfat milk in TBST buffer and incubated with primary antibodies, including anti-Sp1 (1:1000, Millipore), anti-SOD2 (1:1000, GeneTex), anti-MGMT (1:1000, Santa Cruz Biotechnology), anti-phospho-Rad50 (Ser635) (1:1000, Cell Signaling), and anti-tubulin (1:3000, Proteintech) antibodies, for 2 h. After incubation, the membranes were washed with TBST buffer and then incubated with the appropriate secondary antibodies (1:3000, Santa Cruz Biotechnology) for 1 h. Finally, the membranes were washed again and the peroxidase was detected using chemiluminescence with Amersham Hyperfilm ECL (GE Healthcare).
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