Antibodies against H3K27me3 (Cat.# 9733), Ezh2 (Cat.# 5246), and Suz12 (Cat.# 3737) were purchased from Cell Signaling. Antibodies against H3K27ac (Cat.# ab4729) and H3K4me1 (Cat.# ab8895) were purchased from Abcam. Antibodies against α-Tubulin (Cat.# T9026) and FLAG (Cat.# F1804) were purchased from Sigma. Antibody against H3K27M (Cat.# ABE419) was purchased from Millipore. Antibodies against the HA epitope (Clone No. 12CA5) and histone H3 were previously described (Chan et al., 2013a (link); Han et al., 2013 (link)).
Antibodies against α tubulin
Manufactured by Merck Group
Sourced in United States
Antibodies against α-Tubulin are a type of laboratory reagent used in cell biology research. These antibodies specifically bind to the alpha-tubulin protein, which is a key component of the cytoskeleton in eukaryotic cells. They can be used to visualize and study the microtubule network within cells.
Automatically generated - may contain errors
Lab products found in correlation
Ptch1, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
On targetplus smartpool sirna, by Horizon Discovery (1 mentions)
Phospho erk t202 y204, by Cell Signaling Technology (1 mentions)
Gefitinib, by Selleck Chemicals (1 mentions)
Mk 2206, by Selleck Chemicals (1 mentions)
Afatinib, by Boehringer Ingelheim (1 mentions)
Osimertinib, by AstraZeneca (1 mentions)
4 protocols using antibodies against α tubulin
1
Antibodies for Histone Modifications Analysis
2
Antibodies for Hedgehog Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against METTL3 were purchased from Abnova (Taipei, Taiwan). Antibodies against Bak, Bax, Bcl-2, Bcl-xL, GLI1, GLI2, and PTCH1 were purchased from Cell Signaling Technology (Danvers, MA USA). Antibodies against GLI3 and Smo were purchased from Abcam (Cambridge, MA USA). Antibodies against α-Tubulin were purchased from Sigma Aldrich (St. Louis, MO USA).
3
Signaling Pathway Antibody Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-VAMP2 antibodies were from Synaptic Systems (Göttingen, Germany). Monoclonal antibodies against phosphotyrosine were from Becton Dickinson France SAS (Grenoble, France). Polyclonal antibodies against PTP1B were from R&D systems (Minneapolis, MN, USA). Polyclonal antibodies directed against acetylated lysine (pan-acetyl lysine) were from Millipore. Polyclonal antibodies against GLUT4, GLUT1, insulin receptor β subunit and monoclonal antibodies against HSP90 were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phosphospecific antibodies against Akt (Threonine-308 or Serine-473), extracellular signal regulated kinase (ERK)1/2 (Thr-202/Tyr204), and antibodies against Akt, ERK1/2 and Sirt1 were from Cell Signaling Technology (Beverly, MA, USA). Antibodies against α tubulin were from Sigma-Aldrich (St. Louis, MO, USA). Horseradish peroxidase-coupled anti-species antibodies were from Jackson ImmunoResearch Laboratories Europe (Suffolk, UK). Control miRNA and miRIDIAN miR-34a mimic (C-310529-07-0002), and the SMARTpool ON-TARGETplus siRNA against Ptp1b were purchased from Dharmacon (Horizon Discovery).
4
Evaluating Targeted EGFR Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Osimertinib (AZD9291) was provided by AstraZeneca Pharmaceuticals (Cambridge, UK). Afatinib (BIBW 2992) was provided by Boehringer Ingelheim (Ingelheim, Germany). Gefitinib and MK2206 were purchased from Selleck (Houston, TX, USA). All drugs were dissolved in dimethyl sulfoxide (DMSO) to create stocks at a concentration of 10 mM and stored in aliquots at −80 °C. The L-amino acid kit was purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against phosphor-EGFR (Y1068), EGFR, phospho-AKT (S473), AKT, phospho-ERK (T202/Y204), and ERK were purchased from Cell Signaling Technologies (Beverly, MA, USA). Antibodies against α-tubulin were purchased from Sigma-Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!