Factorfour capillary column

Manufactured by Agilent Technologies

Sourced in United States

The FactorFour capillary column is a high-performance gas chromatography column designed for efficient separation and analysis of a wide range of sample components. It features a rugged fused silica construction and proprietary stationary phase coating for reliable and reproducible results.

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Lab products found in correlation

Vf 1ms, by Agilent Technologies (1 mentions) Varian 430 gas chromatograph, by Agilent Technologies (1 mentions) Boron trifluoride methanol, by Merck Group (1 mentions) Varian 430 gc, by Agilent Technologies (1 mentions) Glc 455, by Nu-Chek Prep (1 mentions) Methanol, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions)

5 protocols using factorfour capillary column

PHA Content Analysis by GC

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The PHA content was analyzed using gas chromatography according to the method described earlier (Huijberts et al., 1994). Approximately 10 mg of freeze‐dried cells was mixed with 1 mL chloroform, to which was added 1 mL of methanol solution (containing 15% (v/v) sulfuric acid and 0.4% (w/v) benzoic acid) before incubating the mixture at 100 °C for 3 h to convert the PHA monomers formed to their methyl esters. Subsequently, the mixture was cooled to room temperature, and 0.5 mL of distilled water was added and then shaken for 30 s. The chloroform layer was transferred to another tube and 2 µL sample volume was injected into the gas chromatography column (Varian, Factor Four Capillary Column, Varian, 15 m x 0.25 mm), with injection temperature set at 250 °C and detector temperature at 240 °C. The column temperature for the first 5 min was maintained at 60 °C and then increased at a rate of 3 °C/min to 120 °C. Standard PHB was used for calibration. All of the analysis was performed in triplicate. PHA content (wt% of cell dry weight) of the cells and PHA concentration (per liter culture broth, g/L) were determined as reported previously (Quillaguamán et al., 2007).

Romero Soto L., Thabet H., Maghembe R., Gameiro D., Van‐Thuoc D., Dishisha T, & Hatti‐Kaul R. (2021). Metabolic potential of the moderate halophile Yangia sp. ND199 for co‐production of polyhydroxyalkanoates and exopolysaccharides. MicrobiologyOpen, 10(1), e1160.

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Quantitative Analysis of DiTMP by GC

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Quantitative analysis of the reaction component (DiTMP) was performed using gas chromatography (GC, Varian 430-GC, Varian, USA) equipped with FactorFour Capillary column, VF-1ms (Varian, 15M × 0.25mm) and a flame ionization detector. The initial column temperature was increased from 50 °C to 250 °C at a rate of 20 °C/min. The samples, diluted with acetonitrile, to a concentration of 0.01–0.5 mg/mL, were injected in a split injection mode of 10% at 275 °C. About 10 mg of samples was taken from the reactant during time course, and was dissolved at 10 mg/mL concentration in acetonitrile at room temperature for 20 min. After the solution was centrifuged at 12,000 rpm for 1 min, the resulting supernatant was injected to measure the remaining monomer to GC (Fig. S2). The residual amount and conversion rate of DTMPC in the reaction were calculated by using standard curves of DTMPC concentration. All the data were obtained from two independent experiments and were provided as the average of the replicates with error bars.

Wang P., Park J.H., Sayed M., Chang T.S., Moran A., Chen S, & Pyo S.H. (2018). Sustainable Synthesis and Characterization of Bisphenol A-Free Polycarbonate from Six-Membered Dicyclic Carbonate. Polymer chemistry, 9(27), 3798-3807.

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Cellular Lipid Extraction and Quantification

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Cellular lipids were extracted twice with hexane/2-propanol (3:2). During the first extraction, β-sitosterol was added as an internal standard for quantification. Dried lipids were resuspended in hexane and separated on a Varian Factor Four capillary column, using a Varian 400 GC/MS/MS system (14). The protein concentration after solubilization with 0.5 M NaOH was determined by the BCA protein assay.

Iaea D.B, & Maxfield F.R. (2017). Membrane order in the plasma membrane and endocytic recycling compartment. PLoS ONE, 12(11), e0188041.

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Fatty Acid Methyl Esters Analysis by GC

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FAMEs were analyzed on a Varian-430 gas chromatograph (Varian, Lake Forest, CA, USA) equipped with a Varian FactorFour capillary column (VF-23 ms; 30 m × 0.25 mm i.d. × 0.25 μm film thickness) and a flame-ionization detector. FAMEs were injected in splitless mode. The carrier gas was helium, set to a constant flow rate of 0.7 ml/min. The injector and detector ports were set at 250°C. FAMEs were eluted using a temperature program set initially at 50°C for 2 min, increased at 20°C/min to 170°C held at 170°C for 1 min, increased at 3°C/min to 212°C and held at 212°C for 5 min. Peaks were identified by retention times of authentic FAME standards of known composition (Nu-Chek-Prep, Elysian, MN).

Taha A.Y., Trepanier M.O., Coibanu F.A., Saxena A., Jeffrey M.A., Taha N.M., Burnham W.M, & Bazinet R.P. (2019). Dietary Omega-3 Polyunsaturated Fatty Acid Deprivation Does Not Alter Seizure Thresholds but May Prevent the Anti-seizure Effects of Injected Docosahexaenoic Acid in Rats. Frontiers in Neurology, 9, 1188.

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Lipid Extraction and Fatty Acid Analysis

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After 96 h of treatment, cells were trypsinized, washed twice in PBS and resuspended in fatty acid free medium. Total lipids were extracted as described by Bligh and Dyer [27 (link)] using NaCl, methanol and choloform (Sigma-Alrich) in a 1:2:2 ratio. Thin-layer chromatography was used to separate lipid classes using silica G-plates (EMD Chemical, Gibbstown, NJ, USA) Total phospholipid fatty acids were collected into test tubes and a known amount of 17:0 standard (Avanti, Alabaster, AL, USA) was added. Fatty acids were converted to fatty acid methyl esters (FAME) by incubating for 1 h at 100 °C in hexane and boron trifluoride-methanol (Sigma-Aldrich). FAME were transferred to gas chromatography (GC) vials and samples were analyzed by GC-flame ionization detection (GC-FID) using a Varian-430 GC (Varian, Lake Forest, CA, USA) equipped with a Varian FactorFour capillary column (VF-23 ms; 30 m · 0.25 mm i.d. · 0.25 lm film thickness) and a FID. Samples were injected in splitless mode as previously described [28 (link)]. Fatty acids were identified by comparison to a reference standard consisting of GLC-68 and GLC-455 supplemented with 8:0, 10:0, and 12:0 methyl esters (Nu-Chek Prep, Elysian, MN) and quantified by comparing the area of the peaks to the 17:0 peak. Results are presented as mole percentages of total fatty acids.

Mason J.K., Klaire S., Kharotia S., Wiggins A.K, & Thompson L.U. (2015). α-linolenic acid and docosahexaenoic acid, alone and combined with trastuzumab, reduce HER2-overexpressing breast cancer cell growth but differentially regulate HER2 signaling pathways. Lipids in Health and Disease, 14, 91.

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