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Stemvision

Manufactured by STEMCELL
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STEMvision is a compact, automated imaging system designed for high-speed, high-resolution imaging of cell cultures. It captures detailed, time-lapse images of cells and colonies without the need for manual intervention.

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Lab products found in correlation

Methocult gf m3434, by STEMCELL (2 mentions) Smartdish, by STEMCELL (2 mentions) H4034, by STEMCELL (2 mentions) Cd34 microbead kit, by Miltenyi Biotec (2 mentions) Flt3l, by Thermo Fisher Scientific (1 mentions) Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions) Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions) Stemspan sfem, by STEMCELL (1 mentions) Fetal bovine serum (fbs), by STEMCELL (1 mentions) Human bone marrow, by Lonza (1 mentions) Cd34 microbead kit ultrapure, by Miltenyi Biotec (1 mentions) Methocult h4435, by STEMCELL (1 mentions) Methocult optimum, by STEMCELL (1 mentions) Methocult gf m3434 medium, by STEMCELL (1 mentions) Methocult h4435 enriched, by STEMCELL (1 mentions) Methocult, by STEMCELL (1 mentions) Sh800, by Sony (1 mentions) Cd34 apc cy7, by BioLegend (1 mentions) Methocult 4034, by STEMCELL (1 mentions) Methocult h4034, by STEMCELL (1 mentions)

Close spelling variants of this product

StemVision system (11 citations) StemVision instrument (6 citations) STEMvision™ colony counting software (3 citations) STEMvision™ Hematopoietic Colony Counter (3 citations) StemVision plate reader (3 citations) STEMVision analysis (3 citations) STEMvision™ Human (3 citations) STEMvision instrument and software (3 citations) STEMvision automated CFU colony counter (2 citations) StemVision imaging system (2 citations)

21 protocols using stemvision

1

Quantification of Hematopoietic Progenitor Cells

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FACS sorted KLS cells were plated in different culture conditions in a 96 well plate (4000 cells per condition). At the end of culture, the progeny from each well was plated in two CFU wells with MethoCult GF M3434 (StemCell technologies, Canada). The wells were imaged using StemVision (Stemcell technologies, Canada) and colonies were counted automatically using StemVision analyzer software. The average of the two wells was used to determine the colony count. Four independent experiments were carried out to obtain the final graph (Figure 3f).
Girotra M., Monnard C., Konz T., Sizzano F., Goulet L., Godin J.P., Coukos G., Rezzi S, & Vannini N. (2020). Mineral and Amino Acid Profiling of Different Hematopoietic Populations from the Mouse Bone Marrow. International Journal of Molecular Sciences, 21(17), 6444.
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2

Evaluating Hematopoietic Stem Cell Potential

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For the colony formation assay, CD34+ stem cells of different genotypes were collected and resuspended in the Methocult H4100 media (STEMCELL Technologies; Catalog #04100). This incomplete media were supplemented with IL3 (50 ng/mL; Peprotech), SCF (50 ng/mL; Peprotech), and Flt-3L (50 ng/mL; Peprotech) diluted in StemSpan SFEM (STEMCELL Technologies). Three thousand cells were seeded in one well of a 6-well plate (STEMCELL Technologies; Catalog #27371). The colony formation was visualized using the StemVision (STEMCELL Technologies) 10 days after initial seeding. The colonies were quantified with ImageJ (https://imagej.nih.gov/ij/). Flow cytometry results were analyzed by FlowJo (BD Biosciences).
For plasmacytoid dendritic cell (pDC) differentiation, 8 × 104 CD34+ stem cells were cultured in StemSpan SFEM (STEMCELL Technologies) supplied with TPO (50 ng/mL; Peprotech), Flt-3L (100 ng/mL; Peprotech), IL3 (20 ng/mL; Peprotech), and 1 μmol/L SR-1 (Sigma). The cells were split by half every week for 3 weeks, and on day 21, pDC differentiation was assessed by flow cytometry. Antibodies used for pDC staining (CD123, CD303) are listed in Supplementary Table S2. Flow cytometry results were analyzed by FlowJo (BD Biosciences).
Liu S.Q., Grantham A., Landry C., Granda B., Chopra R., Chakravarthy S., Deutsch S., Vogel M., Russo K., Seiss K., Tschantz W.R., Rejtar T., Ruddy D.A., Hu T., Aardalen K., Wagner J.P., Dranoff G, & D’Alessio J.A. (2020). A CRISPR Screen Reveals Resistance Mechanisms to CD3-Bispecific Antibody Therapy. Cancer immunology research, 9(1), 34-49.
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3

Myeloid Potential Assay of Mouse BM Cells

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Normal or immortalized mouse BM cells were plated at concentrations of 3.3–5 × 104/plate (in duplicate) using MethoCult GF M3434 (STEMCELL Technologies) methylcellulose medium to assess myeloid potential. Colonies were scored manually (and/or using an automated reader, STEMvision; STEMCELL Technologies), total cell numbers were measured at 7 d, and equal numbers of cells were replated using the same conditions.
Basheer F., Giotopoulos G., Meduri E., Yun H., Mazan M., Sasca D., Gallipoli P., Marando L., Gozdecka M., Asby R., Sheppard O., Dudek M., Bullinger L., Döhner H., Dillon R., Freeman S., Ottmann O., Burnett A., Russell N., Papaemmanuil E., Hills R., Campbell P., Vassiliou G.S, & Huntly B.J. (2019). Contrasting requirements during disease evolution identify EZH2 as a therapeutic target in AML. The Journal of Experimental Medicine, 216(4), 966-981.
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4

HSC and MSC Co-culture CFU Assay

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Hematopoietic stem cells (HSCs) were isolated from human bone marrow (Lonza) using the CD34 MicroBead Kit UltraPure (Miltenyi Biotech, Bergisch Gladbach, North Rhine-Westphalia, Germany), frozen, and stored at −180°C. 1×103 HSCs and MSCs were resuspended in 0.1 mL Iscove’s Modified Dulbecco’s Medium, IMDM with 2% FBS (StemCell Technologies, Vancouver, BC, Canada). This HSC:MSC resuspension was added to 1 mL MethoCult H4034 Otimum (StemCell Technologies) and plated in a 6 well SmartDish (StemCell Technologies). CFU assays were quantified on day 14 using the STEMvision (StemCell Technologies) automated colony counter.
Teryek M., Jadhav P., Bento R, & Parekkadan B. (2023). 3D Microcapsules for Human Bone Marrow Derived Mesenchymal Stem Cell Biomanufacturing in a Vertical-Wheel Bioreactor. bioRxiv.
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5

Hematopoietic Stem Cell Assay

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Sorted CD34+ were seeded in methylcellulose, MethoCult H4435 (StemCell Technologies, #04435), in duplicate onto a 6-well SmartDish (StemCell Technologies, #27302) at varying densities of 500 to 1,500 cells. Colonies were scored on a STEMvision after 14 days of incubation (StemCell Technologies) (n=7 and n=5 for shLMNA and sgKLF6, respectively).
Adelman E.R., Huang H.T., Roisman A., Olsson A., Colaprico A., Qin T., Lindsley R.C., Bejar R., Salomonis N., Grimes H.L, & Figueroa M.E. (2019). Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to Leukemia. Cancer discovery, 9(8), 1080-1101.
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6

Lineage-negative cell colony assay

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Lineage-negative cells were treated with 4-Hydroxytamoxifen (1μM) or ethanol (1:1000) for 48 hrs and were then plated in triplicate in MethoCult GF M3434 and incubated for 12 days. Colonies were counted using Stemvision (StemCell Technologies).
Chlon T.M., Stepanchick E., Hershberger C.E., Daniels N.J., Hueneman K.M., Davis A.K., Choi K., Zheng Y., Gurnari C., Haferlach T., Padgett R.A., Maciejewski J.P, & Starczynowski D.T. (2021). Germline DDX41 mutations cause ineffective hematopoiesis and myelodysplasia. Cell stem cell, 28(11), 1966-1981.e6.
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7

CFU Assay for Colony Counting

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The CFU assay was performed by plating 300–900 cells on plate containing MethoCult Optimum (StemCell Technologies) 4 days after electroporation and/or transduction. After 12–16 days, colonies were counted and scored based on their morphological appearance in a blinded fashion using STEMvision (StemCell Technologies). For serial plating, after counting the colonies, cells were harvested and replated with 100,000 cells/plate.
Baik R., Cromer M.K., Glenn S.E., Vakulskas C.A., Chmielewski K.O., Dudek A.M., Feist W.N., Klermund J., Shipp S., Cathomen T., Dever D.P, & Porteus M.H. (2024). Transient inhibition of 53BP1 increases the frequency of targeted integration in human hematopoietic stem and progenitor cells. Nature Communications, 15, 111.
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8

Granulocyte-Monocyte Colony-Forming Assay

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As a validated test for potency, an ISO 15189-accredited granulocyte-monocyte colony-forming unit assay (CFU-GM) assay was used for all washed allogeneic products, with semi-automated counting on STEM Vision (Stemcell technologies, USA) after a 14-day incubation period, as previously reported by our group [9 (link)]. Clonogenicity (%) is calculated as [number of CFU-GM colonies (×104) / post-wash viable CD34+ cells (×106)] x 100.
Mfarrej B., Vicari O., Ouffai S., Malenfant C., Granata A., Thevenet S., Chabannon C., Lemarié C, & Calmels B. (2022). Sepax-2 cell processing device: a study assessing reproducibility of concentrating thawed hematopoietic progenitor cells. Journal of Translational Medicine, 20, 503.
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9

Clonogenic Assay of CD34+ HSPCs

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3 days RNP post-electroporation, 500 CD34+ HSPCs were plated in 1ml methylcellulose media (#H4034, Stem Cell Technologies). Primary CFU-C colonies were counted after 14 days. For the colony replating experiments, 2 weeks after the primary plating, the colonies from two plates were pooled, washed with PBS, and the cells were plated in new methylcellulose media at 25000 cells/ml for an additional 14 days. Images of primary and secondary colonies were taken using StemVision (Stem Cell technologies) using manufacturer’s recommendations.
Bao E.L., Nandakumar S.K., Liao X., Bick A.G., Karjalainen J., Tabaka M., Gan O.I., Havulinna A., Kiiskinen T., Lareau C.A., de Lapuente Portilla A.L., Li B., Emdin C., Codd V., Nelson C.P., Walker C.J., Churchhouse C., de la Chapelle A., Klein D.E., Nilsson B., Wilson P.W., Cho K., Pyarajan S., Gaziano J.M., Samani N.J., Regev A., Palotie A., Neale B.M., Dick J.E., Natarajan P., O’Donnell C.J., Daly M.J., Milyavsky M., Kathiresan S, & Sankaran V.G. (2020). Inherited myeloproliferative neoplasm risk impacts hematopoietic stem cells. Nature, 586(7831), 769-775.
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10

Hematopoietic Stem Cell Manipulation

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HSPCs obtained from Tet2–/– and Ep300Δ/ΔTet2–/– mice were cultured in X-VIVO medium with IL-3 (10 ng/mL), IL-6 (10 ng/mL), and stem cell factor (SCF; 100 ng/mL) overnight and infected with lentiviruses, which express shMyb or scrambled shRNA in a modified PLKO-RFP–expressing vector using a spinfection protocol. Three days after infection, RFP+ cells were sorted and seeded in MethoCult GF M3434 medium (STEMCELL Technologies, 10,000 cells/well). The colonies were counted after 7 days of culture on a STEMvision (STEMCELL Technologies).
Man N., Mas G., Karl D.L., Sun J., Liu F., Yang Q., Torres-Martin M., Itonaga H., Martinez C., Chen S., Xu Y., Duffort S., Hamard P.J., Chen C., Zucconi B.E., Cimmino L., Yang F.C., Xu M., Cole P.A., Figueroa M.E, & Nimer S.D. (2021). p300 suppresses the transition of myelodysplastic syndromes to acute myeloid leukemia. JCI Insight, 6(19), e138478.
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