Albumin diagnostic kit

Manufactured by Wiener Lab

Sourced in Argentina

The Albumin Diagnostic Kit is a laboratory equipment designed to measure the concentration of albumin in biological samples, such as blood or urine. It is a quantitative assay used for the diagnosis and monitoring of various medical conditions where albumin levels are of clinical significance.

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9 protocols using albumin diagnostic kit

Pulmonary Barrier Permeability Assessment

Cited 2 times

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Broncho-alveolar lavages (BAL) samples were obtained as described previously (16 (link), 17 (link)). Briefly, the trachea was exposed and intubated with a catheter, and 2 sequential lavages were performed in each mouse by injecting sterile PBS. The recovered fluid was centrifuged for 10 min at 900 g; and frozen at −70°C for subsequent analyses.
Albumin content, a measure to quantitate increased permeability of the bronchoalveolar–capillarity barrier, and lactate dehydrogenase (LDH) activity, an indicator of general cytotoxicity, were determined in the acellular BAL fluid. Albumin content was determined colorimetrically based on albumin binding to bromcresol green using an albumin diagnostic kit (Wiener Lab, Buenos Aires, Argentina). LDH activity, expressed as units per liter of BAL fluid, was determined by measuring the formation of the reduced form of nicotinamide adenine dinucleotide (NAD) using the Wiener reagents and procedures (Wiener Lab).

Garcia-Castillo V., Tomokiyo M., Raya Tonetti F., Islam M.A., Takahashi H., Kitazawa H, & Villena J. (2020). Alveolar Macrophages Are Key Players in the Modulation of the Respiratory Antiviral Immunity Induced by Orally Administered Lacticaseibacillus rhamnosus CRL1505. Frontiers in Immunology, 11, 568636.

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Quantifying Alveolar-Capillary Barrier Permeability

Cited 3 times

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The albumin content was used as a measure to quantify the increased permeability of the alveolar–capillary barrier, and the activity of the intracellular enzyme lactate dehydrogenase, an indicator of cellular cytotoxicity, was determined in BAL samples [59 (link)]. The albumin content was determined colorimetrically based on albumin binding to bromocresol green using a Wiener-Lab albumin diagnostic kit. LDH activity, expressed as units per liter of BAL, was determined by measuring the formation of the reduced form of NAD⁺ using Wiener’s reagents and procedures (Wiener-Lab, Rosario, Argentina).
The lung wet:dry weight ratio was measured as previously described [37 (link),38 (link)]. Briefly, mice were euthanized and exsanguinated, and their lungs removed, weighed, and dried in an oven at 55 °C for 7 days. After drying, the lungs were weighed again. The wet:dry weight ratio was then calculated as an index of intrapulmonary fluid accumulation, without correction for blood content.

Albarracin L., Ortiz Moyano R., Vargas J.M., Andrade B.G., Cortez Zamar J., Dentice Maidana S., Fukuyama K., Kurata S., Jure M.Á., Kitazawa H, & Villena J. (2022). Genomic and Immunological Characterization of Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Isolates from Northwest Argentina. International Journal of Molecular Sciences, 23(13), 7361.

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Evaluating Lung Barrier Integrity via BAL

Cited 2 times

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Broncho-alveolar lavages (BAL) samples were obtained as described previously [17 (link),22 (link)]. Briefly, the trachea was exposed and intubated with a catheter, and 2 sequential lavages were performed in each mouse by injecting sterile PBS. The recovered fluid was centrifuged for 10 min at 900× g and frozen at −70 °C for subsequent analyses.
Albumin content, a measure to quantitate the increased permeability of the bronchoalveolar–capillarity barrier, and lactate dehydrogenase (LDH) activity, an indicator of general cytotoxicity, were determined in the acellular BAL fluid. Albumin content was determined colorimetrically based on albumin binding to bromcresol green using an Albumin Diagnostic Kit (Wiener Lab, Buenos Aires, Argentina). LDH activity, expressed as units per liter of BAL fluid, was determined by measuring the formation of the reduced form of nicotinamide adenine dinucleotide (NAD) using the Wiener reagents and procedures (Wiener Lab).

Clua P., Tomokiyo M., Raya Tonetti F., Islam M.A., García Castillo V., Marcial G., Salva S., Alvarez S., Takahashi H., Kurata S., Kitazawa H, & Villena J. (2020). The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505. Cells, 9(7), 1653.

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Evaluation of Pulmonary Tissue Injuries

Cited 1 time

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For the evaluation of pulmonary tissue injuries, lungs were excised, washed out with PBS, and immersed in 4% (v/v) formalin saline solution for the histologycal study as previously mentioned [15 (link)]. In addition, biochemical parameters as albumin content and lactate dehydrogenase (LDH) activity were studied in broncho-alveolar lavages (BAL). Albumin content was determined colorimetrically based on albumin binding to bromocresol green using an albumin diagnostic kit (Wiener Lab). LDH activity was determined by measuring the formation of the reduced form of nicotinamide adenine dinucleotide using the Wiener reagents and procedures (Wiener Lab), and was expressed as units per liter of BAL fluid [18 (link)].

Kolling Y., Salva S., Villena J, & Alvarez S. (2018). Are the immunomodulatory properties of Lactobacillus rhamnosus CRL1505 peptidoglycan common for all Lactobacilli during respiratory infection in malnourished mice?. PLoS ONE, 13(3), e0194034.

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Quantifying Alveolar-Capillary Barrier and Cytotoxicity

Cited 2 times

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Albumin content, as a measure to quantify the increased permeability of the alveolar-capillary barrier, and the activity of the intracellular enzyme lactate dehydrogenase, an indicator of cellular cytotoxicity, were determined in BAL samples [22 (link),23 (link)]. Albumin content was determined colorimetrically based on albumin binding to bromocresol green using a Wiener-Lab albumin diagnostic kit. LDH activity, expressed as units per liter of BAL, was determined by measuring the formation of the reduced form of NAD⁺ using Wiener’s reagents and procedures (Wiener-Lab).

Dentice Maidana S., Ortiz Moyano R., Vargas J.M., Fukuyama K., Kurata S., Melnikov V., Jure M.Á., Kitazawa H, & Villena J. (2022). Respiratory Commensal Bacteria Increase Protection against Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infection. Pathogens, 11(9), 1063.

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Quantifying Alveolar Barrier Permeability

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Albumin content, a measure to quantitate increased permeability of the bronchoalveolar–capillarity barrier, and lactate dehydrogenase (LDH) activity, an indicator of general cytotoxicity, were determined in BAL of infected animals as described previously [16 (link), 17 (link)]. Briefly, albumin content was determined colorimetrically based on albumin binding to bromcresol green using an albumin diagnostic kit (Wiener Lab, Buenos Aires, Argentina). LDH activity, expressed as units per liter of BAL fluid, was determined by measuring the formation of the reduced form of nicotinamide adenine dinucleotide (NAD) using the Wiener reagents and procedures (Wiener Lab).

Laiño J., Villena J., Suvorov A., Zelaya H., Ortiz Moyano R., Salva S, & Alvarez S. (2018). Nasal immunization with recombinant chimeric pneumococcal protein and cell wall from immunobiotic bacteria improve resistance of infant mice to Streptococcus pneumoniae infection. PLoS ONE, 13(11), e0206661.

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Quantifying Bronchoalveolar Barrier Permeability

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Protein and albumin content are a measure to quantitate increased permeability of the bronchoalveolar–capillarity barrier, and lactate dehydrogenase (LDH) activity is an indicator of general cytotoxicity. Those parameters were determined in the BAL fluid. Protein content was measured by the bicinchoninic (BCA) protein assay (Pierce Biotechnology Inc., Rockford, IL, USA). Albumin content was determined colorimetry based on albumin binding to bromcresol green using an albumin diagnostic kit (Wiener Lab, Buenos Aires, Argentina). Results were expressed in milligrams per liter of BAL fluid. LDH activity was determined by measuring the formation of the reduced form of nicotinamide adenine dinucleotide (NAD) using commercial reagents and procedures (Wiener Lab). Results were expressed as units per liter of BAL fluid.

Tomotsune K., Raya Tonetti F., Mizuno H., Elean M., Fukuyama K., Zhou B., Ikeda-Ohtsubo W., Nishiyama K., Yamamura A., Karasawa H., Ohnuma S., Horii A., Saito T., Kitazawa H, & Villena J. (2022). The Mucus Binding Factor Is Not Necessary for Lacticaseibacillus rhamnosus CRL1505 to Exert Its Immunomodulatory Activities in Local and Distal Mucosal Sites. International Journal of Molecular Sciences, 23(22), 14357.

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Poly(I:C)-induced Pulmonary Inflammation

Cited 4 times

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Poly(I:C), the TLR3 agonist, was administered as described previously [20 (link),21 (link)]. Briefly, two days after the last day of treatments with HK36, HK39, or HK40, mice received 100 μL of PBS containing 250 μg poly(I:C) (equivalent to 10 mg/kg body weight) through the nasal route. Control mice received 100 μL of PBS. Animals received three doses of poly(I:C) or PBS with 24 h rest period between each administration.
Broncho-alveolar lavages (BAL) samples were obtained as described previously [17 (link),23 (link)]. Albumin content was determined colorimetrically using an albumin diagnostic kit (Wiener Lab, Buenos Aires, Argentina). BAL lactate dehydrogenase (LDH) activity was assessed with the Wiener reagents and procedures (Wiener Lab).
IFN-β (Mouse IFN-beta ELISA Kit), IFN-γ (Mouse IFN-gamma Quantikine ELISA Kit), IL-6 (Mouse IL-6 Quantikine ELISA Kit), IL-10 (Mouse IL-10 Quantikine ELISA Kit), IL-12 (Mouse IL-12 p70 DuoSet ELISA), tumor necrosis factor (TNF)-α (Mouse TNF-α 236 ELISA Kit) and IL-27 (Mouse IL-27 p28/IL-30 Quantikine ELISA Kit) concentrations in BAL samples were measured with commercially ELISA technique kits following the manufacturer’s recommendations (R&D Systems, MN, USA). CCL2 (Mouse MCP1 ELISA Kit (ab208979), and chemokine KC (or CXCL1) were measured with commercially available ELISA technique kits following the manufacturer’s recommendations (Abcam).

Tomokiyo M., Tonetti F.R., Yamamuro H., Shibata R., Fukuyama K., Gobbato N., Albarracin L., Rajoka M.S., Kober A.K., Ikeda-Ohtsubo W., Villena J, & Kitazawa H. (2022). Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity. Cells, 11(19), 2986.

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Comprehensive Lung Function Assessment

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Protein content was measured by the bicinchoninic protein assay (Pierce Biotechnology Inc., Rockford, IL, USA). Albumin content was determined colorimetrically based on albumin binding to bromocresol green using an albumin diagnostic kit (Wiener Lab, Buenos Aires, Argentina).
LDH activity, expressed as units per liter of BAL fluid, was determined by measuring the formation of the reduced form of nicotinamide adenine dinucleotide using the Wiener reagents and procedures (Wiener Lab). The lung wet:dry weight ratio was measured as previously obtained and described by Aeffner et al. [18] . Briefly, mice were euthanized and exsanguinated, and their lungs removed, weighed, and dried in an oven at 55 °C for 7 days. After drying, the lungs were weighed again. Wet:dry weight ratio was then calculated as an index of intrapulmonary fluid accumulation, without correction for blood content.
Whole-lung samples from all experimental groups were excised and washed out with PBS. Then, tissues were immersed in 4 % (v/v) formalin saline solution. Once fixed, samples were dehydrated and embedded in Histowax (Leica Microsystems Nussloch GmbH, Nussloch, Germany) at 56 °C. Finally, lungs were cut into 4 lm serial sections and stained with hematoxylin-eosin for light microscopy examination. All slides were coded and evaluated blindly.

Zelaya H., Tada A., Vizoso-Pinto M.G., Salva S., Kanmani P., Agüero G., Alvarez S., Kitazawa H, & Villena J. (2015). Nasal priming with immunobiotic Lactobacillus rhamnosus modulates inflammation-coagulation interactions and reduces influenza virus-associated pulmonary damage. Inflammation research : official journal of the European Histamine Research Society ... [et al.], 64(8).

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