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3 protocols using c739 gal4

1

DGRP Fly Generation and Maintenance

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DGRP flies were generated and are maintained in our laboratories (Mackay et al. 2012 (link); Huang et al. 2014 (link)). We obtained the OK107-GAL4 line from Dr Tanouye (University of California, Berkeley) and the c739-GAL4, elav-GAL4, repo-GAL4 from the Bloomington Drosophila Stock Center. We also obtained Mi{ET1} lines (CG34113MB04218, CG34113MB04817, CG42313MB08581, Cip4MB03744, cv-cMB03489, cv-cMB03717, DgkMB10383, GefmesoMB10683, Pkc53EMB02781, PkcδMB00303, RhoUMB00991, sideMB07679, trioMB09917) and their co-isogenic control from the Bloomington Drosophila Stock Center. We obtained UAS-RNAi lines (CdGAPrKK100409, CG30440KK101642, CG6424KK107381, Cip4KK101912, cv-cK107255, RhoGAP68FKK102638, RhoUKK112816, roboKK108817, rutKK109441) and the progenitor control y w1118;P{attP,y+w3} line from the Vienna Drosophila Resource Center (Dietzl et al. 2007 (link)). These lines were crossed to elav, repo, or mushroom body–specific GAL4 driver lines to induce targeted gene silencing. All flies were reared on cornmeal–molasses–agar–yeast medium at 25 °C, 70% humidity, and a 12-h light/dark cycle.
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2

Genetic Tools for Drosophila Neuroscience

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Drosophila melanogaster were raised on standard cornmeal food at 25°C and 70% relative humidity on a 12 h:12 h light:dark cycle. The “Cantonized” w1118 w(CS10) was used as the wild-type control. The UAS-shits line used in this study has multiple insertions on the third chromosome. UAS-Dscam[17.1]::GFP flies were obtained from Tzumin Lee. elav-GAL4;+;tub-GAL80ts flies were obtained from Hsueh-Cheng Chiang. The UAS-DenMark flies have been described [45 (link)]. The C305a-GAL4 and C320-GAL4 have also been described [16 (link), 27 (link)]. The 1471-GAL4, C739-GAL4, R42D07-GAL4, R26E01-GAL4, and tub-GAL80ts were obtained from the Bloomington Stock Center. VT49246-GAL4, VT37861-GAL4, VT50658-GAL4, VT41043-GAL4, and VT0765-GAL4 were obtained from the Vienna Drosophila Resource Center, Vienna Tile (VT). UAS-radishRNAi(v39931) and UAS-VGlutRNAi(v104324) were obtained from the Vienna Drosophila RNAi Center; VT30604-GAL4, VT57244-GAL4, and UAS-octβ2RRNAi(v104524) have been described [22 (link)].
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3

Drosophila Rearing and Genetics Procedures

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Drosophila melanogaster flies were raised on standard medium (water, cornmeal, soy bean, agar, molasses, yeast, preservative methyl-4-hydroxybenzoate) at 25 °C, 60% humidity and 14 h/10 h light–dark cycles. Canton-S (CS) served as wild-type Drosophila strain in this study. The following colleagues kindly provided additional stocks: D. Armstrong (189Y-GAL4), M. Heisenberg (wild-type Canton-S, rut1, and dnc1), T.J. Hirsh (Trh493-GAL4), Y. Rao (5HT-1A-GAL4), H. Scholz (UAS-TNT), P. Shen (UAS-npf), H. Tanimoto (H24-GAL4, NP1131-GAL4 and mb247-GAL4; Tub-GAL80ts), and T. Zars (mb247-GAL4). The following lines were obtained from the Bloomington Stock Center: Or83b-GAL4 (#2681), c305a-GAL4 (#30829), c739-GAL4(#7362), npf-GAL4 (#25681), 5HT-1B-GAL4 (#27637), UAS-NaChBac (#9469), UAS-5HT-1ARNAi (#25834 and #33885), UAS-5HT-1BRNAi (#27634 and #54006), UAS-CD4::tdTomato (#35837) and UAS-mCD8::GFP (#5130).
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