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Nipaam

Manufactured by Merck Group
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NIPAAm is a synthetic polymer compound that exhibits temperature-responsive properties. It can be used in various laboratory applications, such as the development of smart hydrogels for controlled drug delivery and tissue engineering. The core function of NIPAAm is to undergo a reversible phase transition in response to changes in temperature, which can be leveraged for a variety of scientific and technical purposes.

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Lab products found in correlation

Diethyl ether, by Avantor (2 mentions) Chloroform, by Avantor (2 mentions) Acetonitrile, by Avantor (2 mentions) Methanol, by Avantor (2 mentions) Super q water system, by Merck Group (2 mentions) Dimethyl γ butyrolactone acrylate dba glycidyl methacrylate gma acrylic acid 2 2 azobis 2 methylpropionitrile azobisisobutyronitrile aibn n n methylenebisacrylamide mba piperazine pip glycine, by Merck Group (2 mentions) Anhydrous 1 4 dioxane, by Avantor (2 mentions) Fetal bovine serum (fbs), by Cambrex (2 mentions) Live dead viability cytotoxicity kit, by Thermo Fisher Scientific (2 mentions) Glutaraldehyde, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Mbaam, by Merck Group (1 mentions) Dimethyl sulfoxide d6, by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Sulfuric acid, by Merck Group (1 mentions) Azobisisobutyronitrile, by Merck Group (1 mentions) Acrylamide am, by Merck Group (1 mentions) Elisa kit, by 4A Biotech (1 mentions) Cell counting kit 8 cck 8, by Beyotime (1 mentions) Calcein am, by Beyotime (1 mentions)

12 protocols using nipaam

1

Synthesis and Purification of PNIPAAm Gels

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Before preparing the monomer solution for PNIPAAm gels, N-isopropylacrylamide (NIPAAm, Sigma-Aldrich, St. Louis, MO, USA) was recrystallized with n-hexane (VWR, International GmbH, Darmstadt, Germany) for purification purposes. For the monomer solution, NIPAAm (689.6 mg), the cross-linker N,N’-methylene-bis-acrylamide (24.1 mg BIS, Sigma-Aldrich, St. Louis, MO, USA) and the photoinitiator lithium phenyl-2,4,6-trimethyl-benzoylphosphinate (18.4 mg LAP, Bio-Techne GmbH, Wiesbaden, Germany) were mixed in deionized water (5 mL) under exclusion of light in a flask and stirred until all solids were dissolved. Then, the solution in the sealed flask was purged with argon to remove oxygen, thereby limiting its influence on the free radical polymerization later on. As previous investigations showed, the homogeneity of photostructured PNIPAAm gels increases upon handling the process under inert Ar atmosphere in a glove box [34 (link)].
Beck A., Obst F., Busek M., Grünzner S., Mehner P.J., Paschew G., Appelhans D., Voit B, & Richter A. (2020). Hydrogel Patterns in Microfluidic Devices by Do-It-Yourself UV-Photolithography Suitable for Very Large-Scale Integration. Micromachines, 11(5), 479.
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2

Osteogenic Hydrogel Scaffold Synthesis

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NiPAAm, dimethyl-γ-butyrolactone acrylate (DBA), glycidyl methacrylate (GMA), acrylic acid (AA), 2,2’-azobis(2-methylpropionitrile) (azobisisobutyronitrile, AIBN), N,N’-methylenebisacrylamide (MBA), piperazine (PiP), glycine, and glutaraldehyde were purchased from Sigma Aldrich (Sigma, St. Louis, MO) and used as received. Anhydrous 1,4-dioxane, diethyl ether, and acetone in analytical grade; and water, acetonitrile, chloroform, and methanol in HPLC-grade were purchased from VWR (Radnor, PA) and used as received. PBS (powder, pH 7.4) was obtained from Gibco Life, Grand Island, NY. Ultrapure water was obtained from a Millipore Super-Q water system (Millipore, Billerica, MA). Acidic gelatin (IEP=5.0) was obtained from Nitta Gelatin (Osaka, Japan). Complete osteogenic medium (COM) was made from minimal essential medium α modification (αMEM) (Gibco Life, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) (Cambrex BioScience, Walkersville, MD), 10−8 M dexamethasone, 10 mM β-glycerol 2-phosphate, 50 mg/L ascorbic acid, and 10 mL/L antibiotic-antimycotic solution (Gibco, Life, Grand Island, NY). Live/Dead viability/cytotoxicity kit was purchased from Molecular Probes (Eugene, OR). The calcium assay was purchased from Genzyme Diagnostics (Cambridge, MA).
Vo T.N., Shah S.R., Lu S., Tatara A.M., Lee E.J., Roh T.T., Tabata Y, & Mikos A.G. (2015). Injectable Dual-Gelling Cell-Laden Composite Hydrogels for Bone Tissue Engineering. Biomaterials, 83, 1-11.
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3

Lignin-Based Hydrogel Synthesis

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Mixed hardwood kraft lignin (LGN) was supplied
by FPInnovations’ pilot plant facility located in Thunder Bay,
ON. NIPAAm (97%), MBAAm (99%), azobisisobutyronitrile (AIBN, 98%),
acetone (97%), dimethyl sulfoxide-d6 (DMSO,
99.9% atom D), and tetramethylthionine chloride (methylene blue) were
obtained from Sigma-Aldrich. Sulfuric acid (98%) and sodium hydroxide
(97%) were also obtained from Sigma-Aldrich and diluted with deionized
water to 20 and 10%, respectively.
Zerpa A., Pakzad L, & Fatehi P. (2018). Hardwood Kraft Lignin-Based Hydrogels: Production and Performance. ACS Omega, 3(7), 8233-8242.
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4

Hydrogel Scaffold Synthesis and Characterization

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NIPAAm, Methylene-N, N-bis(acrylamide) (MBA), ammonium peroxodisulfate (APS), acrylamide (AAm) and acrylamide (AM) were purchased from Sigma-Aldrich. Arg-Gly-Asp (RGD) peptide was obtained from MedChemExpress. Calcein-AM, cell Counting Kit-8 (CCK8) and BCA protein assay kits were purchased from Beyotime Biotech Co., Ltd. All Elisa kits were brought from 4 A Biotech Co., Ltd.
Wan S., Wang K., Huang P., Guo X., Liu W., Li Y., Zhang J., Li Z., Song J., Yang W., Zhang X., Ding X., Leong D.T, & Wang L. (2024). Mechanoelectronic stimulation of autologous extracellular vesicle biosynthesis implant for gut microbiota modulation. Nature Communications, 15, 3343.
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5

Cationic Hydrogel Synthesis and Characterization

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N-isopropylacrylamide (NIPAAm, Sigma-Aldrich, St. Louis, MO, USA, 97%), N-[3-(dimethylamino)propyl]acrylamide (DMAPA, TCI, ≥98.0%), (3-acrylamidopropyl)trimethylammonium chloride (DMAPAQ, Sigma-Aldrich, St. Louis, MO, USA, 75 wt% in H2O), crosslinker N-N’-methylenebis(acrylamide) (NMBA, BeanTown Chemical, 99%), initiator ammonium persulfate (APS, Sigma-Aldrich, St. Louis, MO, USA, ≥98%), catalyst N,N,N’,N’-tetramethylethylenediamine (TEMED, VWR, ≥98%), perfluorooctanoic acid (PFOA, Sigma-Aldrich, St. Louis, MO, USA, 95%) were used as received. Ultrapure water (resistivity 18.2 MΩ) was used in all synthesis reactions and subsequent experiments.
Frazar E.M., Smith A., Dziubla T, & Hilt J.Z. (2022). Thermoresponsive Cationic Polymers: PFAS Binding Performance under Variable pH, Temperature and Comonomer Composition. Gels, 8(10), 668.
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6

Synthesis of Multifunctional Hydrogel Composites

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NIPAAm, N,N′-methylenebis (acrylamide) (BIS), tetramethylenediamine (TEMED) and ammonium persulphate (APS) were procured from Sigma Aldrich Co., Ltd. (Shanghai, China). CuCl2 and Na2S were procured from Shanghai Macklin Biology Technology Co., Ltd. (Shanghai, China). Clay (Mg5.35Li0.66)Si8O20(OH)4(Na0.66) was purchased from Aoyuan new material technology Co., Ltd. (Hebei, China). PVA and CS (degree of deacetylation ≥ 95%) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Beijing, China). Pure water was prepared using a Milli-Q water purification system (Millipore, Bedford, MA, USA).
Chu Y., Chai S., Li F., Han C., Sui X, & Liu T. (2022). Combined Strategy of Wound Healing Using Thermo-Sensitive PNIPAAm Hydrogel and CS/PVA Membranes: Development and In-Vivo Evaluation. Polymers, 14(12), 2454.
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7

Osteogenic Cell Culture Protocols

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NiPAAm, dimethyl-γ-butyrolactone acrylate (DBA), glycidyl methacrylate (GMA), acrylic acid (AA), 2,2’-azobis(2-methylpropionitrile) (azobisisobutyronitrile, AIBN), N,N’-methylenebisacrylamide (MBA), piperazine (PiP), glycine, and glutaraldehyde were purchased from Sigma Aldrich (Sigma, St. Louis, MO) and used as received. Anhydrous 1,4-dioxane, diethyl ether, and acetone in analytical grade; and water, acetonitrile, chloroform, and methanol in HPLC-grade were purchased from VWR (Radnor, PA) and used as received. Phosphate buffered saline (PBS) (powder, pH 7.4) was obtained from Gibco Life, Grand Island, NY. Ultrapure water was obtained from a Millipore Super-Q water system (Millipore, Billerica, MA). Acidic gelatin (isoelectric point = 5.0) was obtained from Nitta Gelatin (Osaka, Japan). Complete osteogenic medium (COM) was made from minimal essential medium α modification (αMEM) (Gibco Life, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) (Cambrex BioScience, Walkersville, MD), 10−8 M dexamethasone, 10 mM β-glycerol 2-phosphate, 50 mg/L ascorbic acid, and 10 mL/L antibiotic-antimycotic solution (Gibco, Life, Grand Island, NY). Live/Dead viability/cytotoxicity kit was purchased from Molecular Probes (Eugene, OR). The calcium assay was purchased from Genzyme Diagnostics (Cambridge, MA).
Vo T.N., Tabata Y, & Mikos A.G. (2016). Effects of Cellular Parameters on the In Vitro Osteogenic Potential of Dual-Gelling Mesenchymal Stem Cell-Laden Hydrogels. Journal of biomaterials science. Polymer edition, 27(12), 1277-1290.
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8

Synthesis of MnCo2O4 Electrode for Alkaline Batteries

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N-isopropylacrylamide (NIPAAm, 97%, Sigma-Aldrich, Poland), N,N′-methylenebisacrylamide (BIS-AAm, 99.5%, Sigma-Aldrich, Poland), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 98%, Sigma-Aldrich, Poland), ammonium persulfate (APS, 98%, Sigma-Aldrich, Poland), N,N,N′,N′-tetramethylethylenediamine (TEMED, 99%, Sigma-Aldrich, Poland), commercially available manganese cobalt spinel MnCo2O4 powder (MCO, Marion Technologie, France), Super P Li conductive carbon black (cCB, Imerys, Belgium), 0.1 M KOH (Titripur®, Merck, Germany).
Pawłowska S., Cysewska K., Ziai Y., Karczewski J., Jasiński P, & Molin S. (2024). Influence of conductive carbon and MnCo2O4 on morphological and electrical properties of hydrogels for electrochemical energy conversion. Beilstein Journal of Nanotechnology, 15, 57-70.
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9

Fabrication of Immunoassay Membranes

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For this study, we purchased polyvinylidene fluoride (PVDF) membranes (Durapore® membrane filter, HVLP02500), NC membranes (Hi-Flow™ Plus HF120), and absorbent pads (SureWick®) from Merck Millipore Ltd. (County Cork, Ireland). We purchased NIPAAm, N,N′-methylenebisacrylamide (BIS), and ammonium peroxodisulfate (APS) from Sigma-Aldrich Japan (Tokyo, Japan), and polydimethylsiloxane (PDMS, SILPOT 184) from Dow Corning Toray Co., Ltd. (Tokyo, Japan). Alkaline phosphatase (ALP) labeled antibody was purchased from Acris Antibodies (AM08098AP-N, San Diego, CA, USA) and 5-bromo-4-chloro-3-indolyl-phosphate/nitroblue tetrazolium (BCIP/NBT) tablets from Sigma-Aldrich Japan (B5655, Tokyo, Japan). We used ultrapure water with a conductivity of 18.2 MΩ·cm in all experiments.
Toda H., Iwasaki W., Morita N., Motomura T., Takemura K., Nagano M., Nakanishi Y, & Nakashima Y. (2022). Reversible Thermo-Responsive Valve for Microfluidic Paper-Based Analytical Devices. Micromachines, 13(5), 690.
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10

Copolymer Synthesis and Composite Carriers

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For copolymer synthesis and formation of composite carriers, N-isopropylacrylamide (NIPAAm), hydroxypropyl methacrylate (HPM), 3-trimethoxysilypropyl methacrylate (TMSPM), and 2-azobisisobutyronitrile (AIBN) were supplied by Sigma. NIPAAm and AIBN were fully recrystallized from n-hexane and ethanol, respectively, and then lyophilized by a freeze dryer. The others were used as received. Solid high-precision glass spheres with diameters of 2.5 mm were provided by Tiancheng Products (Jiangsu, China).
For cell culture, low glucose-Dulbecco's modified Eagle's medium (LG-DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS, Hyclone) and 1% penicillin/streptomycin (P/S, Sigma) was used as the basic culture medium.
Fan X., Wu L., & Yang L. (2021). Fabrication and characterization of thermoresponsive composite carriers: PNIPAAm-grafted glass spheres. e-Polymers, 21(1), 222-233.
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