Fitc concanavalin a con a

Lopinavir was obtained from the National Institutes of Health (NIH) and dissolved in dimethylsulfoxide (DMSO) to obtain a final concentration of 20 mM. Bovine serum albumin (BSA), pepstatin A, ergosterol, lanosterol, fluorescein isothiocyanate (FITC)-labeled secondary antibody, FITC-Concanavalin A (Con A), FITC-Limax flavus agglutinin (LFA), FITC-wheat germ agglutinin (WGA), Calcofluor white, crystal violet, menadione, 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT), formalin, Dulbecco’s Modified Eagle’s Medium (DMEM), Giemsa and cyclophosphamide were purchased from Sigma-Aldrich (St. Louis, MO, USA). Yeast carbon base (YCB) and brain and heart infusion (BHI) media were obtained from HiMedia Laboratories Ltd. (Mumbai, India). Fetal bovine serum (FBS) was purchased from Cultilab (São Paulo, Brazil). Ketamine and xylazine were obtained from União Química Farmacêutica (São Paulo, Brazil). Reagents used in microscopy, electrophoresis and buffer components were purchased from Amersham (Little Chalfont, United Kingdom). All other reagents were of analytical grade.

Indinavir, saquinavir, ritonavir and nelfinavir were obtained from the National Institutes of Health (NIH), and were dissolved in methanol to obtain a final concentration of 20 mM. Bovine serum albumin (BSA), pepstatin A, Tween 80, ergosterol, lanosterol, fluorescein isothiocyanate (FITC)-labeled secondary antibodies, and FITC-Concanavalin A (Con A) and FITC-Sambucus nigra (SNA) agglutinins were purchased from Sigma-Aldrich (St. Louis, MO, United States). Media constituents, reagents used in microscopy and buffer components were purchased from Amersham (Little Chalfont, United Kingdom). All other reagents were of analytical grade.