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Vic mgb nfq

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The VIC/MGB-NFQ is a fluorescent dye used for DNA detection and quantification in real-time PCR applications. It is designed to be compatible with the VIC fluorescent reporter and MGB (Minor Groove Binder) quencher technology. The VIC/MGB-NFQ dye provides a robust signal with high sensitivity and specificity for target DNA detection.

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Lab products found in correlation

7900ht system, by Thermo Fisher Scientific (2 mentions) Fam mgb nfq, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

VIC-MGB (11 citations) MGB-NFQ (2 citations)

2 protocols using vic mgb nfq

1

CD39 Genotyping and CD4+ T Cell Generation

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Peripheral blood mononuclear cells (PBMCs) were obtained from healthy individuals 20–85 years of age. 28 individuals older than 50 years were vaccinated with the VZV vaccine Zostavax. 74 individuals older than 60 years and with known CMV serology were enrolled in a vaccine study with the trivalent influenza vaccine Fluzone in the 2012/2013 season. Individuals were genotyped for the CD39 rs_10748643 SNP by PCR with 0.25 μl specific forward/reverse primers and probes (allele 1: VIC/MGB-NFQ; allele 2: FAM/MGB-NFQ; Applied Biosystems) in quintuplicates in 384-well plates using the ABI 7900HT system. The study was approved by the Stanford institutional review board, and participants gave informed consent. CD39+ T cells were generated by in vitro stimulation of CD4 subsets as described in Supplemental Experimental Procedures.
Fang F., Yu M., Cavanagh M.M., Saunders J.H., Qi Q., Ye Z., Le Saux S., Sultan W., Turgano E., Dekker C.L., Tian L., Weyand C.M, & Goronzy J.J. (2016). Expression of CD39 on Activated T Cells Impairs their Survival in Older Individuals. Cell reports, 14(5), 1218-1231.
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2

CD39 Genotyping and CD4+ T Cell Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells (PBMCs) were obtained from healthy individuals 20–85 years of age. 28 individuals older than 50 years were vaccinated with the VZV vaccine Zostavax. 74 individuals older than 60 years and with known CMV serology were enrolled in a vaccine study with the trivalent influenza vaccine Fluzone in the 2012/2013 season. Individuals were genotyped for the CD39 rs_10748643 SNP by PCR with 0.25 μl specific forward/reverse primers and probes (allele 1: VIC/MGB-NFQ; allele 2: FAM/MGB-NFQ; Applied Biosystems) in quintuplicates in 384-well plates using the ABI 7900HT system. The study was approved by the Stanford institutional review board, and participants gave informed consent. CD39+ T cells were generated by in vitro stimulation of CD4 subsets as described in Supplemental Experimental Procedures.
Fang F., Yu M., Cavanagh M.M., Saunders J.H., Qi Q., Ye Z., Le Saux S., Sultan W., Turgano E., Dekker C.L., Tian L., Weyand C.M, & Goronzy J.J. (2016). Expression of CD39 on Activated T Cells Impairs their Survival in Older Individuals. Cell reports, 14(5), 1218-1231.
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