Acetonitrile

Dithiothreitol, ethylenediamine tetraacetic acid (EDTA), iodoacetamide, nicotinamide (NAM), quagolomycin A (TSA), tetraethylammonium bromide (TEAB) and urea were purchased from Sigma (Santa Clara, CA). Acetonitrile, C18 columns (Waters, Milford, Massachusetts, USA), distilled water (Fisher Chemical, Waltham, Massachusetts, USA), formic acid (Fluka, Santa Clara, CA, USA), protease inhibitor (Calbiochem, Darmstadt, Germany), trifluoroacetic acid (Sigma-Aldrich, Santa Clara, CA, USA), and trypsin (Promega, Madison, Wisconsin, USA) were utilized in the experiments. The BCA assay kit was supplied by Beyotime biotechnology (Shanghai, China). The TMT kit and mass calibration standards were produced by Thermo Fisher Scientific (Waltham, Massachusetts, USA). Additional chemicals were purchased in China.

The reagents used for the SO₂ test were methyl red, 30% hydrogen peroxide solution, hydrochloric acid, 0.01 N sodium hydroxide solution (Factor 1.00, Wako, Tokyo, Japan), ethanol (High-performance liquid chromatography (HPLC) grade, J. T. Baker, Phillipsburg, NJ, USA), and nitrogen gas (purity 99.995%). SO₂ was extracted as the test apparatus using a Monier-Williams transformation apparatus and an automatic potentiometer (T50M, Mettler-Toledo, Zurich, Switzerland) was used.
Aflatoxin B1, B2, G1, and G2 mixed solutions (Aflatoxin Mix, Sigma, Burlington, MA, USA) were used as standards for the mycotoxin test. Pididium hydrobromic acid perbromate (PBPB, Sigma, USA) was used as the derivatization reagent; methanol (HPLC grade, J. T. Baker, USA), acetonitrile (HPLC grade, J. T. Baker, USA), acetic acid (HPLC grade, J. T. Baker, USA), and Tween 20 were used as solvents. (Bio-Rad, Hercules, CA, USA); and an immunoaffinity column (AflaTest, Vicam, Manchester, TN, USA) was used.
The standard used for the benzopyrene test was benzopyrene (Sigma, USA). Furthermore, 3-methylcholanthrene (Sigma, USA) was used as an internal standard, and n-hexane (HPLC grade, J. T. Baker, USA), acetonitrile (HPLC grade, J. T. Baker, USA), anhydrous sodium sulfate (HPLC grade, J. T. Baker, USA), dichloromethane (J. T. Baker, USA), and a Florisil cartridge (1 g, Sep-Pak^® Waters, Bedford, MA, USA) were used.

The bicinchoninic acid (BCA) protein assay kit was purchased from Pierce (Rockford, IL); hydrazide support and sodium periodate were purchased from Bio-Rad (Hercules, CA); sequencing-grade trypsin was purchased from Promega (Madison, WI); PNGase F was purchased from New England Biolabs (Ipswich, MA); Sep-Pak C18 1 cc Vac Cartridges were purchased from Waters (Milford, MA); and acetonitrile (ACN), trifluoroacetic acid (TFA), formic acid, urea, tris(2-carboxyethyl)phosphine (TCEP), iodoacetamide, phorbol 12-myristate 13-acetate (PMA), 5 M NaCl, sodium dodecyl sulfate (SDS), 10× phosphate buffered saline buffer (PBS) pH 7.4 and 1 M Tris–HCl pH 8.0 buffer were purchased from Sigma-Aldrich (St. Louis, MO).