The largest database of trusted experimental protocols

2 protocols using eagle s modified essential medium

1

Cell Lines Used for SARS-CoV-2 Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
Grivet (Chlorocebus aethiops) kidney epithelial Vero (American Type Culture Collection [ATCC]; CCL-81) and Vero E6 (BEI Resources; NR596), Vero E6/TMPRSS2 (JCRB Cell Bank, Japan; JCRB1819), human colorectal adenocarcinoma Caco-2 (ATCC; HTB-37), HEK293T (ATCC; CRL-3216), human hepatocarcinoma HepG2 (ATCC; #HB-8065), human hepatocarcinoma Huh-7 (a gift from NIH/National Institute of Allergy and Infectious Diseases [NIAID]/Rocky Mountain Laboratories, Hamilton, Montana, USA), human lung adenocarcinoma Calu-3 (ATCC; HTB-55), and human lung carcinoma A549 cells (a gift from University of Rochester Medical Center) were maintained at 37°C and 5% CO2 in DMEM (Life Technologies) containing 10% heat-inactivated FBS. Primary human hepatocytes from an anonymous 41-year-old woman with colorectal cancer metastasized to the liver were obtained from Liver Center Resources, Pittsburgh Liver Research Center, Pittsburgh, Pennsylvania, USA, and maintained at 37°C and 5% CO2 in Eagle’s modified essential medium (Gibco).
+ Open protocol
+ Expand
2

CRC Organoid and Cell Line Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumour resection specimens were processed into single-cell suspension by mechanical and enzymatic digestion, mixed with Matrigel® (Corning, USA) and incubated in 50µl droplets on 24-well plates, as previously described by Sato et al. (38 (link)). Organoid culture media (OCM) Human Intesticult™ (Stemcell Technologies Inc, Canada) containing 0.2% Primocin™ (In vivogen, USA) and passaged once confluent. Isogenic KRAS HCT116 (Horizon Discovery, Cambridge, UK) and C80 (#12022904-1VL, Sigma Aldrich, USA) CRC cell lines were cultured in McCoy’s 5a Medium (Gibco, USA) with 10% fetal bovine serum (FBS, Gibco, USA), Iscove’s modified Dulbecco’s media (Gibco. USA) containing 10% FBS, 2mM L-Glutamine (Gibco, USA) and Eagles modified essential medium (Gibco, USA) with 2mM L-Glutamine containing 1% non-essential amino acids (Sigma-Aldrich, USA), respectively, and one hundred units/ml penicillin and 100 μg/ml streptomycin (Gibco, USA). Cells were passaged when sub-confluent at a split ratio of 1 in 3. All cultures were tested for mycoplasma using EZ-PCR Mycoplasma Test Kit (Biological Industries, Israel).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!