Donepezil, clioquinol, HSA, ThT, 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride (EDC), N‐hydroxysuccinimide (1‐hydroxypyrrolidine‐2,5‐dione, NHS), and FITC‐dextran were purchased from Sigma–Aldrich; GM1 was purchased from J&K(Beijing); TAT peptide and Aβ1‐42 were purchased from GL Biochem (Shanghai), and the purity of the peptides was greater than 98%; glutaric dialdehyde was purchased from Aladdin (Shanghai); hexafluoroisopropanol (HFIP) and 4‐(N‐maleimidomethyl) cyclohexane‐1‐carboxylic acid 3‐sulfo‐N‐hydroxysuccinimide ester sodium salt (Sulfo‐SMCC) were purchased from Yuanye (Shanghai). FITC‐transferrin, FITC‐cholera toxin subunit B, and LysoTracker Red probe were purchased from Invitrogen (USA). Milli‐Q water (18 MΩ cm) was used in the experiments. Nicotinic acetylcholine receptors α7(α7 nAChR), SYAP1 and GAP43 antibody were purchased from Bioss (Beijing). Rat cysteine protease‐3 ELISA kit was purchased from Huyu Biotechnology (Shanghai).
1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride
Manufactured by Merck Group
Sourced in United States, China
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is a water-soluble carbodiimide compound commonly used as a coupling agent in various chemical and biological applications. It is a versatile reagent that facilitates the formation of amide bonds between carboxyl and amine groups.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide, by Merck Group (22 mentions)
Phosphate buffered saline (pbs), by Merck Group (4 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (3 mentions)
N hydroxysuccinimide nhs, by Merck Group (3 mentions)
Toluene, by Thermo Fisher Scientific (3 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (3 mentions)
4 dimethylaminopyridine, by Merck Group (3 mentions)
4 morpholineethanesulfonic acid, by Merck Group (3 mentions)
Polyacrylic acid, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Dspe peg2000 crgd, by Xi’an Ruixi (3 mentions)
N hydroxysuccinimide nhs, by Sinopharm (2 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (2 mentions)
4 dimethylaminopyridine dmap, by Merck Group (2 mentions)
2 n morpholino ethanesulfonic acid, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Bca protein assay kit, by Beyotime (2 mentions)
Hexane, by Thermo Fisher Scientific (2 mentions)
2 amino 2 hydroxymethyl 1 3 propanediol, by Merck Group (2 mentions)
42 protocols using 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride
1
Protein Conjugation and Cellular Uptake Assay
2
Synthesis of PLGA-PEG Copolymer
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Triethylamine and dichloromethane were dried with calcium hydride before use as described previously (26 (link)). Carboxylate-functionalized copolymer PLGA-PEG was synthesized by the conjugation of PLGA-COOH and NH2-PEG-COOH. Briefly, 200 mg PLGA-COOH, 2.9 mg 1-(3-Dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (Sigma-Aldrich; Merck KGaA), 1.8 mg N-Hydroxysuccinimide (NHS; Sinopharm Chemical Reagent Co., Ltd.) and 1 mg 4-dimethylaminopyridine (Sino-pharm Chemical Reagent Co., Ltd.) were dissolved in 10 ml dichloromethane, and stirred for 24 h at room temperature to convert PLGA-COOH to PLGA-NHS. Then, 34 mg NH2-PEG-COOH and 8.6 µl triethylamine were added into the solution. After further stirring for 8 h at room temperature, dichloromethane was removed through the use of a rotary evaporator and 5 ml N,N-Dimethylformamide (Sino-pharm Chemical Reagent Co., Ltd.) was added to dissolve PLGA-PEG. Then, the solution was added to a dialysis bag (width, 44 mm; MW cut-off, 8,000-14,000); unreacted PEG and other small molecules were removed by dialyzing for 3 days, and then PLGA-PEG was freeze-dried for 24-48 h.
3
Synthesis and Characterization of Hyaluronic Acid-Based Drug Delivery System
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Branched PEI (MW =25 kDa) was purchased from Sigma-Aldrich (St Louis, MO, USA). HA (MW =79 kDa) was purchased from Freda (Shandong, People’s Republic of China). Doxorubicin⋅HCl (DOX⋅HCl) was purchased from Dalian Meilun Biology Technology Co. Ltd. (Dalian, People’s Republic of China). N-hydroxysuccinimide, HBA and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride were purchased from Sigma-Aldrich. Hyals were purchased from Sigma-Aldrich. The plasmid pCMV-EGFP (pEGFP-N1) carrying enhanced green fluorescent protein (EGFP) under cytomegalovirus (CMV) promoter was propagated in Escherichia coli and purified by Endo Free Plasmid Maxi Kit (Qiagen, Hilden, Germany). The purity and concentration of pDNA was then measured using NanoDrop UV–Vis Spectrophotometers (ND-2000C; Thermo Fisher Scientific, Waltham, MA, USA). Hoechst 33342 was purchased from Thermo Fisher Scientific. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was also obtained from Sigma Aldrich. GoldView was purchased from BioTeke Corporation (Beijing, People’s Republic of China). All other reagents were of commercial special grade and were used without further purification.
4
Rapid Quantification of Phytochemicals
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PBZ, CAR, ovalbumin (OVA), bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), Tween-20, Freund’s adjuvants (complete and incomplete), culture media RPMI-1640, hypoxanthine aminopterin thymidine (HAT), hypoxanthine thymidine (HT), N-hydroxysuccinimide (NHS), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), 3,3,5,5-tetramethylbenzidine (TMB), polyethylene glycol 20,000 (PEG 20,000, 50%), goat anti-mouse IgG-horseradish peroxidase (goat anti-mouse IgG-HRP), N-propylethylenediamine (PSA), and graphitized carbon black (GCB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate, N-N dimethylformamide (DMF), ethyl acetate, chloroauric acid (HAuCl4), and 3-hydroxytyramine hydrochloride (DA·HCl) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The sample pad, absorbent paper, and polyvinylchloride (PVC) backing pads were purchased from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). The nitrocellulose (NC) membrane was purchased from Sartorius Steadi Trading Co., Ltd. (Shanghai, China). Orange, grape, and cabbage mustard samples were purchased from the Tian Hong supermarket, (Nanchang, China). The 96-well polystyrene microplates and 6-well microplates were purchased from NEST Biotechnology Co., Ltd. (Wuhan, China).
5
Synthesis and Characterization of Curcumin-Loaded Nanoparticles
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Curcumin and (ACP)-GPLGIAGQr9-(ACP) were purchased from Hangzhou Guang Lin Biological Pharmaceutical Co. Ltd. (Hangzhou, China) and China Peptides Co. Ltd. (Shanghai, China), respectively. 1,2,3-Propanetricarboxylic acid was obtained from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). N,N’-Dicyclohexylcarbodiimide (DCC), ε-caprolactone (ε-PCL), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), stannous 2-ethylhexanoate [Sn(Oct)2], mPEG (MW=1900), pentaerythritol (PET), and 4-dimethylaminopyridine (DMAP) were purchased from Sigma-Aldrich (Shanghai, China).
Dialysis bags (MWCO=14 kDa) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gene Star Co. (Shanghai, China) and Thermo Fisher Scientific Suzhou Co., Ltd (Suzhou, China), respectively. Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biotechnology Co.,Ltd. (Huzhou, China). Mouse embryonic fibroblast L929 cells and human glioma U251 cells were provided by Zhejiang Provincial Center for Disease Control and Prevention (Hangzhou, China).
Dialysis bags (MWCO=14 kDa) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gene Star Co. (Shanghai, China) and Thermo Fisher Scientific Suzhou Co., Ltd (Suzhou, China), respectively. Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biotechnology Co.,Ltd. (Huzhou, China). Mouse embryonic fibroblast L929 cells and human glioma U251 cells were provided by Zhejiang Provincial Center for Disease Control and Prevention (Hangzhou, China).
6
Folate-Targeted Chitosan Nanoparticles
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Perfluorooctyl bromide (PFOB), folic acid (FA), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were provided by Sigma-Aldrich (USA), LutrolF68 (F68) was obtained from BSAF (Germany), egg lecithin (PL-100M) was provided by AVT (Japan), chitosan (CS) (m.w. 25–50 kg/mol, degree of deacetylation 95%) was obtained from Haidebei Marine Bioengineering Co., Ltd. (China), folic acid PEG acid (FA-PEG-COOH) (m.w. 3400 g/mol) was supplied by Nanocs (USA), glycerol was purchased from Hunan Erkang Pharmaceutical Co. Ltd. (China), folic acid was provided by Aladdin (China), fluorescein isothiocyanate (FITC) was obtain from Solarbio (China), N-hydroxysuccinimide (NHS) and N,N′-dicyclohexylcarbodiimide (DCC) were supplied by Sinopharm Chemical Reagent Co., Ltd. (China), the dialysis membrane (MwCO = 8000–14000) was provided by Sigma-Aldrich (USA), Spectra/Gel Absorbent was obtained from spectrum (USA), water was purified using system from Millipore (France), and other chemicals and solvent were of analytical grade. Trypsin, fetal bovine serum (FBS), RPMI1640, and folate-free RPMI1640 media were purchased from Gibco (USA), cells were supplied by ATCC (USA), and CellTiter96® Aqueous One Solution Cell Proliferation Assay (MTS) was obtained from Promega Corporation (USA).
7
Azaperone Immunoassay Development
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Azaperone, azaperol, haloperidol, spiperone,
droperidol, methyl 4-phenylbutanoate, 4-chlorobutanol chloride, aluminum
trichloride, potassium iodide, potassium carbonate, and 1-(pyridin-2-yl)
piperazine were purchased from J&K Scientific Ltd. (Beijing, China).
The 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC),
1-hydroxypyrrolidine-2,5-dione (NHS), KLH, OVA, carbon disulfide,
acetonitrile, tetrahydrofuran (THF), dichloromethane (DCM), N,N-dimethylformamide (DMF), Freund’s
complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA),
and horseradish peroxidase (HRP)-labelled goat antimouse IgG were
all purchased from Sigma-Aldrich (St. Louis, MO, USA).
Nitrocellulose
(NC) high-flow-plus membrane (Pura-bind RP) was obtained from Whatman-Xinhua
Filter Paper Co. (Hangzhou, China). Polyvinylchloride (PVC) backing
cards, sample pads (CB-SB08), and absorption pads (SX18) were supplied
by Goldbio Tech Co. (Shanghai, China). The coating antigen and mAb
against azaperone were obtained from our laboratory.
droperidol, methyl 4-phenylbutanoate, 4-chlorobutanol chloride, aluminum
trichloride, potassium iodide, potassium carbonate, and 1-(pyridin-2-yl)
piperazine were purchased from J&K Scientific Ltd. (Beijing, China).
The 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC),
1-hydroxypyrrolidine-2,5-dione (NHS), KLH, OVA, carbon disulfide,
acetonitrile, tetrahydrofuran (THF), dichloromethane (DCM), N,N-dimethylformamide (DMF), Freund’s
complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA),
and horseradish peroxidase (HRP)-labelled goat antimouse IgG were
all purchased from Sigma-Aldrich (St. Louis, MO, USA).
Nitrocellulose
(NC) high-flow-plus membrane (Pura-bind RP) was obtained from Whatman-Xinhua
Filter Paper Co. (Hangzhou, China). Polyvinylchloride (PVC) backing
cards, sample pads (CB-SB08), and absorption pads (SX18) were supplied
by Goldbio Tech Co. (Shanghai, China). The coating antigen and mAb
against azaperone were obtained from our laboratory.
8
Biomimetic Hydrogel Fabrication
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Gelatin (type B from bovine skin), gellan, soluble elastin from bovine neck ligament, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), and phosphate buffered saline (PBS) were supplied by Sigma-Aldrich (Milan, Italy). The peptide sequences Gly-Gly-Gly-Arg-Glu-Asp-Val (GGGREDV) and Gly-Gly-Gly-Gly-Arg-Gly-Asp-Ser-Pro (GGGGRGDSP) were synthesized and provided by Cambridge Research Biochemicals (Billingham, Cleveland, UK). Moreover, calcium chloride and acetone were purchased from Carlo Erba Reagenti (Milan, Italy). All the other reagents were commercially available and used as received.
9
Synthesis and Characterization of Oxi-αCD Nanocarrier
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α-Cyclodextrin (α-CD) was provided by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 4-(hydroxymethyl) phenylboronic acid pinacol ester, 4-dimethylaminopyridine (DMAP), 1,1′-carbonyldiimidazole (CDI), tetrahydrofuran (THF), dimethyl sulfoxide (DMSO), acetone and Pluronic F127 were supplied by Sigma–Aldrich Co., Ltd. (Shanghai, China). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy (polyethylene glycol)-2000 (DSPE-PEG2000), folic acid-conjugated DSPE-PEG-3400 (DSPE-PEG3400-FA) and cRGD-conjugated DSPE-PEG-2000 (DSPE-PEG2000-cRGD) were acquired from Xi’an Ruixi Biological Technology Co., Ltd. (Xi’an, China). Lecithin (refined) was supplied by Alfa Aesar (Shanghai, China). Cy5 free acid was purchased from Lumiprobe, LLC (Hallandale Beach, FL, United States). 4′,6-Diamidino-2-phenylindole (DAPI) was obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Oxi-αCD and Cy5-conjugated Oxi-αCD were synthesized according to our previously reported strategy (Wang et al., 2019 (link)). All chemicals were of analytical grade and used without further purification. Ultrapure water was used throughout this study.
10
Formulation and Characterization of Doxorubicin-Loaded Liposomes
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Polyethylenimine (PEI, MW, 600 g/mol), glutaric anhydride (GA), 1-[3-(dimethylamino) propyl]-3-ethylcarbodiimide hydrochloride (EDC), and N-hydroxy-succinimide (NHS) were purchased from Sigma Aldrich Co. (Milwaukee, WI, USA). Chemicals 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), l -α-phosphatidylcholine (soy-hydrogenated) (HSPC), and cholesterol (CHOL) were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA). The anticancer drug doxorubicin (DOX) was obtained from Boryung Pharm. Co. (Ansan, Korea) and calcein was purchased from Sigma Co. (St. Louis, MO, USA). All other materials and solvents were of analytical grade and used without further purification.
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