FWA-GFP in the central cell and endosperm was detected using an Olympus BX53 fluorescence microscope equipped with an Olympus DP80 digital camera [57 (link)]. DRE2-GFP in the root was detected using the Nikon’s modular A1+/A1R+ confocal laser scanning microscope system (National Center for Protein Sciences at Peking University, Beijing).
Dp80digital
Manufactured by Olympus
Sourced in Japan
The DP80Digital is a high-performance digital camera designed for use in microscopy applications. It features a large 8-megapixel sensor and supports high-resolution image capture. The camera is capable of capturing detailed images with excellent color reproduction and low noise levels. It is designed to be compatible with a wide range of microscopes and scientific imaging software.
Automatically generated - may contain errors
10 protocols using dp80digital
1
Fluorescence Microscopy for Protein Localization
2
Histological Analysis of Liver Tissue
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The hematoxylin and eosin (HE) staining was performed on the liver tissue using standard protocols previously described by Cui et al. (2020). Thus, the tissues (um/g) were fixed for 24 h and embedded with paraffin. Thereafter, the tissue sections were selected and used for histological and morphological observations. Frozen sections of the liver tissue were carbowax-embedded and oil-red staining was performed, and then all the sections were viewed under fluorescence microscope and photos were taken (DP80Digital, Olympus, Tokyo, Japan).
3
Histological Analysis of Liver Tissue
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The fixed liver tissues and AATs were dehydrated with an alcohol gradient and were made transparent with xylene. The transparent samples were embedded in paraffin, cut into 5 mm slices, and routinely stained with hematoxylin and eosin (H&E) and Oil Red O. All the sections were viewed under an electronic microscope (DP80Digital, Olympus, Tokyo, Japan), and then ten fields were randomly selected for statistical analysis.
4
Histological Analysis of Chicken Liver
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Six chickens (6) from each group were randomly selected and euthanized, and their liver tissues were collected. The left lobe liver was selected and the vernier caliper was used to ensure the samples were collected from the same tissue areas. Six (6) liver tissue samples were obtained from each group, however, three (3) liver samples (μm/g) were fixed with 4 % paraformaldehyde for 24 h and dehydrated with different concentrations of alcohol. Thereafter, the tissue was embedded in paraffin, cut into thin slices (3–5 µm) and placed on a slide. After hematoxylin and eosin (HE ) staining, the HE sections were sealed with neutral resin. Then, the remaining three (3) fresh liver samples were cut in the size of 24 mm × 24 mm × 2 mm, frozen and then were placed on a tissue bearer dripping with an OTC embedding agent. Slices (thickness 10 µm) were obtained with the slicing machine (Leica CM1520, Germany) and affixed to the anti-slip slide. After staining with oil red O staining solution for 15 min, the slices were sealed with a neutral resin. Six sections (3 HE sections and 3 oil red O staining sections) per treatment were used for the data collection. All sections were viewed under a microscope (DP80Digital, Olympus, Tokyo, Japan) and ten fields were randomly selected for statistical analysis.
5
Freshwater Algae Culture Collection Protocols
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The 47 specimen strains described in this study were isolated from water samples and deposited in the Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB collection), Wuhan, Hubei province, China. Voucher numbers are shown in Table S1 . Samples were isolated from the field by the authors and primarily examined under a stereoscope microscope and an Olympus BX53 (Tokyo, Japan) light microscope equipped with an Olympus DP80 digital camera. CellSens standard image analysis software (Tokyo, Japan) was used for morphological examination. Characteristics of the 47 specimens are summarized in Table 1 .
6
Organ Morphology Analysis in Hens
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After 8 weeks of the experiment, 6 hens were taken from each experimental group, the livers and ovaries were dissected, washed with normal saline, and weighed to calculate the organ index = organ weight/body weight, and the liver and ovarian tissues were placed in more than 4% fixed in a paraformaldehyde solution. Liver and ovarian fragments were then dehydrated in graded ethanol, cleared in xylene, and embedded in paraffin. Embedded tissue samples were sectioned at 5 μm and mounted on glass slides and stained with Hematoxylin and eosin following standard protocols. The morphological observation was performed using an optical microscope (DP80Digital, Olympus, Tokyo, Japan) and Image Pro plus analysis.
7
Histological Evaluation of Pectoralis Major Muscle
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Anterior pectoralis major muscle was fixed in 10% neutral buffered formalin, trimmed, processed through Sakura Tissue-Tek VIP 5, and embedded in paraffin. Tissue sections (4–5 um) were stained with hematoxylin and eosin (H&E ) and Masson's Trichrome stains using an adapted chicken breast muscle specific protocol (Vanhatalo et al., 2021 ). Images were captured using a BX43F microscope fitted with a DP80 digital and cellSens Dimension software v.1.12 (Olympus). Muscle histopathology scores were based on macrophage infiltration, tissue damage, adipose cell and collagen presence as follows: Mild (some macrophage infiltration), Moderate (few macrophage phagocytosis and appearance of fibrotic tissue), and Severe (high levels of macrophage infiltration with complete muscle destruction) (Figure S1 E–L) (Kuttappan et al., 2013 (link)).
8
Oil Red O Staining of Granulosa Cells
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GC Oil red O staining was performed according to the manufacturer’s protocol. Briefly, GCs were washed in a phosphate buffered saline (PBS) and fixed with ORO Fixative for 30 min, the stationary fluid was discarded and then washed twice in PBS. Thereafter, 60% isopropanol was added and maintained for 5 min, then ORO Stain was added and retained for 20 min. Furthermore, Mayer Hematoxylin staining solution was added and incubated for 2 min after the GCs were washed 5 times. This was followed by another washing of the GCs followed by the addition of ORO Buffer and sustained for 1 min. After washing with PBS, the GCs were covered with distilled water. All sections and GCs were viewed under an electronic microscope (DP80Digital, Olympus, Tokyo, Japan) and ten fields were randomly selected for statistical analysis.
9
Histological Analysis of Liver Tissue
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Hematoxylin and eosin (HE ) staining was performed on liver tissue using standard protocols previously described by Cui et al. (2020) and Amevor et al. (2021) (link). The tissues (µm/g) were fixed for 24 h and embedded in paraffin. Thereafter, tissue sections were selected for histological and morphological observations. Frozen sections of the liver tissue were carbowax-embedded, oil-red staining was performed, and then all the sections were viewed under a fluorescence microscope and photos were taken (DP80 Digital, Olympus, Tokyo, Japan).
10
Histomorphological Analysis of Liver Tissues
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The tissues (um/g) were fixed for 24 hours and embedded with paraffin. After hematoxylin and eosin (HE) staining, the tissue sections were used for histological and morphological observation. Moreover, the frozen sections of the liver tissues were carbowax-embedded and stained by oil red O. All the sections were observed with a fluorescence microscope (DP80Digital, Olympus, Tokyo, Japan), and then ten fields were randomly selected for statistical analysis.
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