Murashige skoog media

Mating assays were performed on Murashige-Skoog media (Sigma, USA) and V8 juice media plus 100 μg/mL myo-inositol (pH 5.0) (Sigma, USA) using KN99a as the MATa partner. Strains were co-inoculated in equal quantities via toothpick and incubated in the dark at room temperature for two weeks. Filamentation and sporulation was examined and imaged using a Leica M125 stereomicroscope fitted with a Leica DFC 425c digital camera running Leica Application Suite 3.6 software (Leica, Germany).

Arabidopsis seedlings were plated onto Murashige-Skoog media (Sigma, St. Louis, MO) supplemented with 0.8% agar, 3% sucrose (MS agar) and appropriate antibiotics. After stratification in the dark at 4°C for 2 days, plates were transferred to a growth room with a 16L:8D cycle (80 μmol m⁻² s⁻¹) at 22°C. Luminescence recordings were performed on a TopCount (Packard Bioscience, Shelton, CT) over 7 days, as previously described [10 (link)]. The data were analyzed by fast Fourier transform–nonlinear least squares (FFT–NLLS) [71 (link)] using the interface provided by the Biological Rhythms Analysis Software System version 3.0 (BRASS) (http://www.amillar.org).