Aevertaid first strand cdna synthesis kit

Unless otherwise stated, all chemicals were obtained from the Sigma Chemical Company, St. Louis, MO, United States. Mouse anti-rat TfR1 (transferrin receptor 1) and fetal bovine serum (FBS) were purchased from Invitrogen, Carlsbad, CA, United States; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, United States; rabbit polyclonal anti-Ft-L and anti-DMT1 (divalent metal transporter 1, SLC11A2) from Protein-tech, Chicago, IL, United States; and rabbit polyclonal anti-phospho-STAT3 (Tyr705) and mouse monoclonal anti-STAT3 both from Cell Signaling Technology, Inc., Danvers, MA, United States. Recombined mouse IL-6 protein was obtained from Gene Script, Piscataway, NJ, United States; IL-6 enzyme-linked immunosorbent assay (ELISA) kits from R&D Systems, Minneapolis, MN, United States; goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibodies from LI-COR Bio Sciences, Lincoln, NE, United States; TRIzol reagent from Life Technologies, Carlsbad, CA, United States; AevertAid First Strand cDNA Synthesis Kit from Thermo Scientific, Waltham, MA, United States; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, United States; and BCA protein Assay kit and protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China.

Unless otherwise stated, all chemicals were obtained from Sigma Chemical Co., St. Louis, MO, USA. Mouse monoclonal anti-rat transferrin receptor 1 (TfR1) and goat anti-mouse IgG-Alexa 488 were purchased from Invitrogen, Carlsbad, CA, USA; rabbit polyclonal anti-rat divalent metal transporter 1 (DMT1, SLC11A2) and rabbit polyclonal anti-ferritin-heavy-chain (Ft-L) from Proteintech, Chicago, IL, USA; rabbit polyclonal anti-ferritin-hight-vhain (Ft-H) from Bioworld Technology Inc., Louis Park, MN, USA; and rabbit polyclonal anti-mouse ferroportin 1 (Fpn1) from Novus Biologicals, Littleton, CO, USA. Goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody was bought from Li-Cor, Lincoln, NE, USA; TRIzol reagent from Life Technologies, Carlsbad, CA, USA; and AevertAid First Strand cDNA Synthesis Kit and BCA Protein Assay Kit both from Thermo Scientific, Waltham, MA, USA. Microtubule associated protein 2 (MAP2) and astrocyte marker glial fibrillary acidic protein (GFAP) were obtained from Chemicon International, Inc. Temecula, CA, USA; the in situ cell death detection kit, TMR red from USA Roche, Nutley, NJ, USA. The Health Department of Hong Kong and Shanghai Government and the Animal Research Ethics Committee of The Chinese University of Hong Kong and Fudan University approved the experimental procedures of this study.

Unless otherwise stated, all chemicals including mouse monoclonal anti-β-actin were obtained from Sigma-Aldrich Chemical Co., St. Louis, MO, U.S.A. The recombinant mouse HGF protein was obtained from R&D Systems, Minneapolis, MN, U.S.A.; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, U.S.A.; mouse anti-human TfR1 from Invitrogen Life technologies, Carlsbad, CA, U.S.A.; rabbit polyclonal anti-ferritin light chain (Ft-L) and anti-DMT1 from Proteintech, Chicago, IL, U.S.A.; and rabbit monoclonal anti-IRP1 and anti-IRP2 were from Abcam, San Francisco, CA, U.S.A. Goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibodies were bought from LI-COR Bio Sciences, Lincoln, NE, U.S.A.; the bicinchoninic acid (BCA) protein assay kit and AevertAid First Strand cDNA Synthesis Kit from Thermo Fisher Scientific, Waltham, MA, U.S.A.; the TRIzol reagent from Life technologies, Carlsbad, CA, U.S.A.; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, U.S.A.; protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China. Recombinant HGF protein was dissolved in phosphate buffer saline (PBS) and diluted to 1–120 ng/ml before use.