Anti tslp antibody

SSA and SSC were purchased from Sigma-Aldrich (St. Louis, MO, USA). TNF-α was obtained from ProSpec-Tany TechnoGene Ltd. (Ness Ziona, Israel). The firefly luciferase assay system was purchased from Promega (Madison, WI, USA). The web-based program MatInspector (http://www.genomatix.de/ (accessed on 17 December 2020)) was used for promoter analysis. Anti-TSLP antibody was obtained from Novus Biologicals (Centennial, CO, USA), and anti-EGR1, phospho-ERK1/2 (Thr202/Tyr204), phospho-p38 (Thr180/Tyr182), and phospho-JNK1/2 (Thr183/Tyr185) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). The anti-GAPDH antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies conjugated to Alexa Fluor 488 and rhodamine red-X were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). The MAPK, p38 kinase, and JNK inhibitors (U0126, SB203580, SP600125), DNCB, TB, and an H&E staining kit were purchased from Sigma-Aldrich.

Chrysin, DNCB, TB, and H&E staining kits were purchased from Sigma-Aldrich (St. Louis, MO, USA). TNFα was purchased from ProSpec-Tany TechnoGene, Ltd. (Ness-Ziona, Israel). A firefly luciferase assay system was obtained from Promega (Madison, WI, USA). Anti-TSLP antibody was obtained from Novus Biologicals (Centennial, CO, USA), and phospho-ERK1/2 (Thr202/Tyr204), phospho-p38 (Thr180/Tyr182), and phospho-JNK1/2 (Thr183/Tyr185) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH and anti-EGR1 antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). A secondary antibody conjugated to rhodamine red-X was obtained from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).