Apoptosis was assessed using the ANNEXIN V-FITC/PI cell apoptosis detection kit (BB-4101, Bestbio). LECs in the different experimental groups were trypsinized without EDTA, suspended in binding buffer, and incubated with Annexin V-FITC for 15 min, followed by PI staining for 5 min. Flow cytometry (Canto2, BD, USA) was then performed to analyze the results.
Annexin 5 fitc pi cell apoptosis detection kit
Manufactured by BestBio
Sourced in China
The ANNEXIN V-FITC/PI cell apoptosis detection kit is a laboratory product designed to detect and quantify apoptosis in cell samples. It contains Annexin V-FITC and propidium iodide (PI) reagents, which are used to stain cells and identify different stages of the apoptotic process.
Automatically generated - may contain errors
Lab products found in correlation
Rt master mix, by Takara Bio (2 mentions)
Il 1β, by GeneCopoeia (2 mentions)
Tnf α, by GeneCopoeia (2 mentions)
Gapdh, by GeneCopoeia (2 mentions)
Real time pcr primer col 1, by GeneCopoeia (2 mentions)
Runx 2, by GeneCopoeia (2 mentions)
Il 18, by GeneCopoeia (2 mentions)
Canto 2, by BD (1 mentions)
Pg lps, by Merck Group (1 mentions)
Sybr mixture, by Takara Bio (1 mentions)
Beta actin antibody, by Proteintech (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Sybr mixture, by CWBIO (1 mentions)
3 protocols using annexin 5 fitc pi cell apoptosis detection kit
1
Quantifying Apoptosis in Lens Epithelial Cells
2
Necroptosis Regulation in Bone Cells
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Pg-LPS was bought from Sigma (St. Louis, MO, USA). Rabbit RIP1, MLKL and beta-actin antibody was from Proteintech (USA), and mouse RIP3 antibody was from Santa (USA). Real-time PCR primer COL-1, OCN, RUNX-2, BSP, TNF-α, IL-1β, IL-18 and GAPDH were from Genecopoeia (USA), SYBR mixture was from Takara (Japan), RT Master Mix was from Takara (Japan). Inhibitor of Nec-1 (ab141053) was from Abcam (USA). Annexin V-FITC/PI: Cell apoptosis detection kit was from Bestbio Company (China). Nude mice were bought from Animal Center of the Fourth Military Medical University Animal Center.
3
Molecular Mechanisms of Cell Death
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LPS was bought from Sigma (St. Louis, MO, USA). Rabbit RIP3, RIP1, MLKL, and β-action antibody were from Proteintech (Chicago, USA). Real-time PCR primer COL1, OCN, RUNX-2, BSP, TNF-α, IL-1β, IL-18, and GAPDH were from Genecopoeia (USA), and SYBR mixture was from CWBIO Company (China). RT Master Mix was from Takara (Japan). Inhibitor Z-VAD-fmk (ab120382) was from Abcam and GSK’872(2673-5) was bought from BIOVISION. The Annexin V-FITC/PI cell apoptosis detection kit was from Bestbio Company (China). Anti-vimentin antibody (ab8978), Anti-pan Cytokeratin antibody (ab7753), and Alexa Fluor 647(ab150171) were bought from Abcam and Goat Anti-Mouse IgG and Cy3 was bought from Zhuangzhibio (Xi’an, China).
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