Anti taci pe

CP-25 was supplied by the Chemistry Laboratory of Institute of Clinical Pharmacology of Anhui Medical University (Hefei, Anhui Province, China). Rituximab (Roche Pharma, Switzerland); Etanercept (Shanghai Guojian Pharmaceutical, Co., Ltd., China); BAFF (Pepro Tech, United States); anti-CD19-APC/PE/FITC, anti-CD20-FITC, anti-CD27-PE/APC, anti-BAFFR-APC, anti-BCMA-PE, and anti-TACI-PE (Biolegend, United States); anti-β-actin (ZSGB-BIO, China); Anti-TRAF2 (Santa Cruz, China); Anti-Phospho-NF-κBp65, anti-p100/52, and anti-Phospho-p38 MAPK (Cell Signaling Technology, United States); RPMI 1640 medium (Gibco, United States); fetal bovine serum (Zhejiang Tianhang Biotechnology, China).

PBMCs (1 × 10⁶ cells) were incubated with anti-CD19-PEDy647, anti-CD38-PE, anti-CD41a-FITC, anti-CD41a-PE (Immunotools, Friesoythe, Germany), anti-CD19-PECy7, anti-CD27-APC, anti-BAFFR-FITC, anti-TACI-PE (BioLegend, San Diego, USA), anti-CD86-PE, anti-IgG-PE, anti-IgD-FITC (BD), anti-IgA-FITC, and anti-IgM-PE (Dako, CA, USA) mAbs and the corresponding isotype controls. Whole blood (100 μl) was incubated with anti-CD4-PECy7 (BioLegend), CD5-PE (BD Biosciences), CD19-PEDy647, and CD41a-FITC (Immunotools). Red blood cells were lysed, and white cells fixed using BD FACS lysing solution (BD Biosciences) to be analyzed by flow cytometry.