Whole cell lysates were prepared in RIPA buffer and loaded onto SDS-PAGE. Western blotting was performed using following antibodies: anti-MITF C5 (Millipore, MAB3747), anti-human GTF2H1 (AbD, MCA4041Z), anti-c-Myc (Cell Signaling, #5605), anti-human CDK7 (Cell Signaling, #2916; Santa Cruz, sc-365075), anti-human phospho-CDK7 T170 (Abcam, ab155976), anti-human MAT-1 (Santa Cruz, sc-6234), anti-human cyclin H (Abcam, ab92376), anti-human CDK2 (Santa Cruz, sc-53220), anti-human RNA polymerase II (Bethyl, A300-653A), anti-human phospho-RNA polymerase II (S2) (Bethyl, A300-654A), anti-human phospho-RNA polymerase II (S5) (Bethyl, A300-655A), anti-human p27 (Santa Cruz, sc-528), anti-HA-tag HRP conjugate (Cell Signaling, #2999), anti-FLAG (Sigma Aldrich, F1804), and anti-β-Actin (Sigma Aldrich, A2228). Anti-mouse immunoglobulins/HRP (Dako, P0447) and anti-rabbit immunoglobulins/HRP (Dako, P0448) were used as secondary antibodies. Proteins were visualized with Amersham ECL solution (GE Healthcare, RPN2106).
Seoane M., Buhs S., Iglesias P., Strauss J., Puller A.C., Müller J., Gerull H., Feldhaus S., Alawi M., Brandner J.M., Eggert D., Du J., Thomale J., Wild P.J., Zimmermann M., Sternsdorf T., Schumacher U., Nollau P., Fisher D.E, & Horstmann M.A. (2019). Lineage-specific control of TFIIH by MITF determines transcriptional homeostasis and DNA repair. Oncogene, 38(19), 3616-3635.
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