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α chrna4 antibody

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The α-CHRNA4 antibody is a highly specific and sensitive tool for the detection of the alpha-4 subunit of the nicotinic acetylcholine receptor (nAChR) in various experimental systems. This antibody can be used for applications such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of the α-CHRNA4 subunit.

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Lab products found in correlation

Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (2 mentions) Chemidoc mp system, by Bio-Rad (2 mentions) Goat anti mouse igg, by Agilent Technologies (2 mentions) α chrnb2 antibody, by Alomone (2 mentions) α adra2a antibody, by Merck Group (2 mentions) α chrna5 antibody, by Novus Biologicals (2 mentions) α chrnb4 antibody, by Alomone (2 mentions) Monoclonal mouse α pactin antibody, by Merck Group (2 mentions) Goat α rabbit igg, by Cell Signaling Technology (2 mentions)

2 protocols using α chrna4 antibody

1

Western Blot Analysis of MafA Variants

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Western blot analysis with MafAWT and MafARIP islets was performed as previously described (Rosengren et al., 2010 (link)). Primary antibodies were α-CHRNB2 antibody (1:200; no. ANC-012; Alomone Labs), α-ADRA2A antibody (1:500; no. A271; Sigma-Aldrich), α-CHRNA5 antibody (1:400; no. NBP1–69122; Novus Biologicals), α-CHRNA4 antibody (1:500; no. ANC-004; Alomone Labs), α-CHRNB4 antibody (1:500; no. ANC-014; Alomone Labs), and monoclonal mouse α-pactin antibody (1:2,000; Sigma). Secondary antibodies were goat α-rabbit IgG (1:1,000; Cell Signaling Technology) and goat anti-mouse IgG (1:2,000; Dako). Signals were visualized using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific) and Chemi-Doc MP system (Bio-Rad).
Ganic E., Singh T., Luan C., Fadista J., Johansson J.K., Cyphert H.A., Bennet H., Storm P., Prost G., Ahlenius H., Renström E., Stein R., Groop L., Fex M, & Artner I. (2016). MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes. Cell reports, 14(8), 1991-2002.
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2

Western Blot Analysis of MafA Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis with MafAWT and MafARIP islets was performed as previously described (Rosengren et al., 2010 (link)). Primary antibodies were α-CHRNB2 antibody (1:200; no. ANC-012; Alomone Labs), α-ADRA2A antibody (1:500; no. A271; Sigma-Aldrich), α-CHRNA5 antibody (1:400; no. NBP1–69122; Novus Biologicals), α-CHRNA4 antibody (1:500; no. ANC-004; Alomone Labs), α-CHRNB4 antibody (1:500; no. ANC-014; Alomone Labs), and monoclonal mouse α-pactin antibody (1:2,000; Sigma). Secondary antibodies were goat α-rabbit IgG (1:1,000; Cell Signaling Technology) and goat anti-mouse IgG (1:2,000; Dako). Signals were visualized using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific) and Chemi-Doc MP system (Bio-Rad).
Ganic E., Singh T., Luan C., Fadista J., Johansson J.K., Cyphert H.A., Bennet H., Storm P., Prost G., Ahlenius H., Renström E., Stein R., Groop L., Fex M, & Artner I. (2016). MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes. Cell reports, 14(8), 1991-2002.
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